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Ogarkov O.,Research Institute of Epidemiology and Microbiology | Mokrousov I.,St Petersburg Pasteur Institute | Sinkov V.,Irkutsk Regional Diagnostic Center | Zhdanova S.,Research Institute of Epidemiology and Microbiology | And 2 more authors.
Infection, Genetics and Evolution | Year: 2012

An interaction of different human alleles and endemic bacterial strains may be clinically manifested as different outcome of the disease in different hosts infected with the same genotype. The primary objective of this study was to investigate this issue in the model of Mycobacterium tuberculosis and human DC-SIGN encoding CD209 promoter SNP (rs4804803) in Russian Siberian population. We sought to find a possible combination of M. tuberculosis lineage and human host allele/genotype correlating with unfavorable outcome of the disease. The 101 paired DNA samples from patients with pulmonary TB (human and M. tuberculosis DNA) were studied by 12-loci MIRU-VNTR typing (M. tuberculosis strains) and CD209 -336 A/G typing (human DNA). Ninety autopsy DNA samples as a source of human and mycobacterial nucleic acids from persons who died from TB were also subjected to the same genotyping procedures. A human control group consisted of 177 healthy individuals. The Beijing genotype was more frequently identified in autopsy versus patient samples, in 70.0% and 51.5%, respectively (χ 2=6.06, P=0.01). Regarding other M. tuberculosis genetic families, no significant difference in LAM family distribution among patient strains and autopsy samples has been found. In contrast, Ural genotype was significantly less frequently detected in the autopsy samples (χ 2=6.12, P=0.01). Allelic and genotypic frequencies of the CD209 -336A/G did not differ significantly under global comparison when contrasting controls versus patients versus autopsy samples. However intriguing and contrasting significant associations were found in the male subgroup under M. tuberculosis genotype-stratified comparisons. Firstly, male carriers of -336AA genotype were more frequently infected with Beijing genotype (χ 2=5.2, P=0.02). Secondly and remarkably, this association was inverted in the autopsy sample: male carriers of -336AA genotype died less frequently due to TB caused by a Beijing rather than a non-Beijing strain (χ 2=5.37, P=0.02). In conclusion, we hypothesize that although carriers of CD209 -336A allele are more sensitive to infection with a Beijing strain, a combination of human CD209 -336G allele and M. tuberculosis Beijing genotype leads more frequently to the lethal outcome in pulmonary TB male patients in Russian (Caucasian) population. © 2011 Elsevier B.V.. Source

Petrov N.V.,Saint Petersburg State University of Information Technologies, Mechanics and Optics | Pavlov P.V.,Zhukovsky Gagarin Air Force Academy | Malov A.N.,Irkutsk State Medical University
Quantum Electronics | Year: 2013

Using the equations of scalar diffraction theory we consider the formation of an optical vortex on a diffractive optical element. The algorithms are proposed for simulating the processes of propagation of spiral wavefronts in free space and their reflections from surfaces with different roughness parameters. The given approach is illustrated by the results of numerical simulations. © 2013 Kvantovaya Elektronika and Turpion Ltd. Source

Illarionova E.A.,Irkutsk State Medical University | Nikonovich O.L.,Irkutsk State University
Bulletin of the Russian Academy of Sciences: Physics | Year: 2016

The possibility of applying spectrophotometry to the quantitative determination of metronidazole and tinidazole with pyrocatechol violet as an optical reference sample is demonstrated. The optical parameters of the absorption bands of pyrocatechol violet and the optimum regions of absorption are established. Conversion factors are determined and the equations of calibration curves are presented. © 2016, Allerton Press, Inc. Source

Kozlov Y.,Surgery Academy | Novozhilov V.,Irkutsk State Medical University
Journal of Laparoendoscopic and Advanced Surgical Techniques | Year: 2015

Purpose: The aim of this study was the comparison of single-port and multiport laparoscopic methods for the treatment of inguinal hernia in children during the first 3 months of life. Subjects and Methods: Between January 2002 and December 2012, we performed 260 laparoscopic operations in neonates and infants with the diagnosis of inguinal hernia. Surgical procedures were single-port endoscopic herniorraphy (Group I; 180 patients) and multiport laparoscopic herniorraphy (Group II; 80 patients). The two groups were compared for demographics of patients, operative report, and early and late postoperative outcomes. Results: We applied the single-port laparoscopic method for correction of inguinal hernia in small babies. The mean operative time (duration of one-sided and bilateral repair) in Group I was 16 minutes, whereas the mean duration of the operation in Group II was 15.73 minutes. The number of doses of postoperative analgesia was 1.19 and 1.22, respectively. The length of hospital stay was 8.12 hours in Group I and 8.27 hours in Group II. No differences between the two groups were recorded in the follow-up period: no recurrence in either group and hydrocele formation in only 1 patient in Group II. Conclusions: There are similar functional results in treatment of inguinal hernia in babies during the first 3 months of the life using single- and multiport laparoscopy. Scarless cosmetic results are seen in the single-incision laparoscopic surgery group. © Copyright 2015, Mary Ann Liebert, Inc. 2015. Source

Kozlov Y.,Irkutsk State Medical University | Novozhilov V.,Surgery Academy
Journal of Laparoendoscopic and Advanced Surgical Techniques | Year: 2015

Purpose: Thoracoscopic plication of the diaphragm is an alternative to conventional surgical treatment of diaphragmatic evisceration via thoracotomy in neonates and infants. The aim of this study is to compare results of treatment by these two methods in the past 11 years. Materials and Methods: We studied the data of 35 neonates who underwent standard posterolateral thoracotomy (18 patients; Group I) or video-assisted thoracoscopic surgery (17 patients; Group II) for diaphragmatic plication. The two groups were compared for patient demographics, operative reports, and postoperative parameters. Results: The groups were similar in terms of demographics and preoperative parameters. There was a significant difference in mean operative time between the open and thoracoscopic procedure (71.67 minutes versus 51.76 minutes; P<.05). Duration of care in the neonatal intensive unit and length of hospital stay were significantly shorter in Group II (5.89 days versus 3.23 days [P<.05] and 13.06 days versus 9.88 days [P<.05]). Early postoperative complications (hemothorax, pneumothorax) were frequent in the thoracotomy group (Group I) (16.67% versus 0%; P>.05). Recurrence of diaphragmatic evisceration was observed only in the thoracotomy group (11.11% versus 0%; P=.486). Conclusions: Thoracoscopic plication of the diaphragm in infants in the first 3 months of life demonstrates better results than open surgery. © Mary Ann Liebert, Inc. 2015. Source

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