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Afanas'ev M.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Mironova L.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Balakhonov S.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East
Molecular Genetics, Microbiology and Virology | Year: 2015

General questions of the methodology of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-ToF MS-analysis, a new technology in laboratory diagnostics of infections), as well as a number of special questions concerning the use of this technology in the identification and typing of causative agents of extremely dangerous infections (plague, cholera, and tularemia), are considered in the present review. The problems of sample preparation for study and the ensurance of biological safety are discussed. © 2015, Allerton Press, Inc. Source


Mironova L.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Afanas'ev M.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Goldapel E.G.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Balakhonov S.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East
Molecular Genetics, Microbiology and Virology | Year: 2015

Allelic polymorphism of the housekeeping genes (dnaE, lap, recA, pgm, gyrB, cat, chi, and gmd) from Vibrio cholerae strains of differing epidemiological significance (n = 41) isolated in Siberia and the Far East during the seventh cholera pandemic was studied. Irrespective of time and source of isolation, all toxi-genic strains isolated in the period of epidemic complications were characterized by an identical allelic profile and belonged to the same sequence-type. Nine sequence types were detected in nonepidemic isolates. Their dendrogram clustering was associated with the serogroup and, in some cases, with the territory and time of isolation. The structural heterogeneity of the nontoxigenic V. cholerae housekeeping genes was, in most cases, caused by synonymous nucleotide replacements (Dn/Ds < 1). This indicates the prevalence of negative selec-tion at the analyzed genome regions of V. cholerae. The revealed differences in the housekeeping gene struc-tures of V. cholerae of different epidemiological significance can be regarded as evidence of different direc-tions of evolution of these strain groups. © 2015, Allerton Press, Inc. Source


Mironova L.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Balakhonov S.V.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Urbanovich L.Ya.,Irkutsk Antiplague Research Institute of Siberia and the Far East | Kozhevnikova A.S.,Irkutsk Antiplague Research Institute of Siberia and the Far East | And 3 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2012

PCR detection of biovar specificity and pathogenicity determinants has been performed in order to analyze the structural components of the genome of V. cholerae El Tor strains (n = 90) isolated during epi- demic outbreaks in Siberia and the Far East, and the nucleotide sequence of the ctxB gene and the structure of ctxAB gene promoter region have been determined. As a result, toxicogenic strains V. cholerae El Tor were divided into two groups: the first group contained strains isolated at the initial stages of the seventh pandemia (in the 1970s), and they had the genotype ctxB3+rstR El+rstRCl-rstC+TCL+tbr4; all El Tor vibrios posing an epidemic risk isolated in the 1990s were characterized as atypical variants due to ctxB gene harboring of the classical genotype (ctxB) in their genome and were placed in the second group. The second group fell into three genotypes according to the set of tested genetic markers (ctxB, rstR, rstC, TLC, and tbr) and affect cer- tain territories. The established variability of the genome structure of Vibrio El Tor atypical variants may serve as a marker for molecular and epidemiological analysis of cholera entry pathways and distribution and is use- ful for speculating on the most likely directions of pathogen evolution. © Allerton Press, Inc., 2012. Source

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