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Dentovskaya S.V.,State Research Center for Applied Microbiology | Ivanov S.A.,State Research Center for Applied Microbiology | Kopylov P.K.,State Research Center for Applied Microbiology | Shaikhutdinova R.Z.,State Research Center for Applied Microbiology | And 5 more authors.
Acta Naturae | Year: 2015

It has recently been shown that the NlpD lipoprotein is essential to Yersinia pestis virulence and that subcutaneous administration of the nlpD mutant could protect mice against bubonic and pneumonic plague better than the EV vaccine strain [PLoS One 2009. V. 4. No. 9. e7023]. In this study, similar ΔnlpD mutants were generated on the basis of other Y. pestis parent strains, including strains from the subspecies microtus, which is avirulent to guinea pigs and humans. Comparative testing confirmed that immunization of mice with ΔnlpD mutants induces immunity 105 times more potent than the one induced by the administration of the EV vaccine strain. At the same time, NlpD- bacteria failed to protect guinea pigs in the case of a subcutaneous challenge with Y. pestis, inducing a 106 times less potent protection compared with that conferred by immunization with the EV vaccine strain. The possible causes of the observed phenomena are discussed. © 2015 Park-media, Ltd. Source

Prozorova G.F.,Favorsky Irkutsk Institute of Chemistry | Korzhova S.A.,Favorsky Irkutsk Institute of Chemistry | Pozdnyakov A.S.,Favorsky Irkutsk Institute of Chemistry | Emelyanov A.I.,Favorsky Irkutsk Institute of Chemistry | And 2 more authors.
Russian Chemical Bulletin | Year: 2015

The water-soluble nanocomposite with the uniform distribution of silver nanoparticles in polymer matrix was synthesized on the basis of poly-1-vinyl-1,2,4-triazole. Physicochemical and immunomodulatory properties of the nanocomposite were studied. The stimulating effect of the nanocomposite on the functional capability of phagocytes in vitro and potential of using it as a drug, which increases the natural resistance of organism against infectious agents, were established. © 2015 Springer Science+Business Media New York. Source

Kulikalova E.S.,Irkutsk Antiplague Research Institute of Siberia and Far East | Sappo S.G.,Irkutsk Antiplague Research Institute of Siberia and Far East | Urbanovich L.Y.,Irkutsk Antiplague Research Institute of Siberia and Far East | Markov E.Y.,Irkutsk Antiplague Research Institute of Siberia and Far East | And 2 more authors.
Contemporary Problems of Ecology | Year: 2014

Vibrio cholerae eltor strains with different epidemic importance isolated from river water in the city of Vladivostok during a cholera outbreak (1999) and in the city of Irkutsk during a safe cholera period (2005) are used in the experiment. A biofilm structure consisting of a peripheral part, bundles, polysaccharide matrix, canals, and polymorphic vibrios is presented by light and luminescent microscopy. The metachromatic pink coloring of the matrix (crystal violet and toluidine blue) or fluorescent reddish orange color (acridine orange) are evidence of acid mucopolysaccharide (glucosaminoglycans) content. The biofilm of a toxigenic strain as opposed to a nontoxigenic one is formed much later, while the elements comprising its structure are more apparent. The viability of vibrio cells during the experiment (90 days) preserving the initial pathogenic potential testifies to the highly adaptable properties of the Vibrio cholerae eltor, which promotes its survival and existence in surface water reservoirs under favorable ecological conditions (optimal temperature, existence of chitin-containing substratum, etc.). © 2014 Pleiades Publishing, Ltd. Source

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