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Markov E.Y.,Irkutsk Antiplague Research Institute of Rospotrebnadzor | Kulikalova E.S.,Irkutsk Antiplague Research Institute of Rospotrebnadzor | Urbanovich L.Y.,Irkutsk Antiplague Research Institute of Rospotrebnadzor | Vishnyakov V.S.,Russian Academy of Sciences | Balakhonov S.V.,Irkutsk Antiplague Research Institute of Rospotrebnadzor
Biochemistry (Moscow) | Year: 2015

The role of chitin and its hydrolysis products generated by Vibrio cholerae chitinases in mechanisms of its adaptation in water environments, metabolism, preservation, acquisition of pathogenic potential, and its epidemiological value are reviewed. Chitin utilization by V. cholerae as a source of energy, carbon, and nitrogen is described. Chitin association promotes biofilm formation on natural chitinous surfaces, increasing V. cholerae resistance to adverse factors in ecological niches: the human body and water environments with its inhabitants. Hydrolytic enzymes regulated by the corresponding genes result in complete chitin biodegradation by a chitinolytic catabolic cascade. Consequences of V. cholerae cell and chitin interaction at different hierarchical levels include metabolic and physiological cell reactions such as chemotaxis, cell division, biofilm formation, induction of genetic competence, and commensalic and symbiotic mutual relations with higher organisms, nutrient cycle, pathogenicity for humans, and water organisms that is an example of successful interrelation of bacteria and substratum in the ecology of the microorganism. © 2015 Pleiades Publishing, Ltd.

Afanasiyev M.V.,Irkutsk Antiplague Research Institute of Rospotrebnadzor | Ostiyak A.S.,Irkutsk Antiplague Research Institute of Rospotrebnadzor | Balakhonov S.V.,Irkutsk Antiplague Research Institute of Rospotrebnadzor
Klinichescheskaya Laboratornaya Diagnostika | Year: 2014

The study of sampling of strains of Y. pestis of main and altaic subspecies was implemented. The modern technique of identification of microorganisths was applied using MALDI-TOF mass spectrometry analysis. The evaluation of biological safety of method of sampling preparation was implemented. To supplement the identification base "BioTyper" the spectrum of typical strains of Y. pestis were obtained. The enhanced identification base was used to evaluate possibilities of application of MALDI-TOF technology for identification and taxonomic differentiation of Y. pestis from other representatives of genus of Yersinia. In the process of study a complete concordance of results of mass spectrometry identification and classic cultural method was observed On the basis of mass spectrometry characteristic of analyzed sampling the differentiation between strains of Y. pestis of subspecies pestis and strains of subspecies altaica was implemented. The study results testify the effectiveness of application of mass spectrometry analysis for reliable interspecies and intraspecific differentiation of plague agent. The simplicity and velocity of sampling preparation and implementation of analysis and low cost of active storage allow considering the MALDI-TOF technology of mass spectrometry identification as highly perspective method for laboratory diagnostic of plague agent.

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