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Farzadfar S.,Iranian Research Institute of Plant Protection IRIPP | Pourrahim R.,Iranian Research Institute of Plant Protection IRIPP
Australasian Plant Disease Notes | Year: 2017

Symptomatic alfalfa leaf samples were collected from Western Iran. RT-PCR was performed using degenerate primers resulting in amplification of a fragment approximately 600 bp in size corresponding to the coat protein (CP) gene of luteoviruses. BLAST analysis of the CP nucleotide sequences revealed the highest similarity (96–97%) with Turnip yellows virus (TuYV) isolates. The present study shows for the first time the occurrence of TuYV in Iran. © 2017, Australasian Plant Pathology Society Inc.

Dashtbozorgi Z.,Islamic Azad University at Tehran | Ramezani M.K.,Iranian Research Institute of Plant Protection IRIPP | Waqif-Husain S.,Islamic Azad University at Tehran
Analytical Methods | Year: 2013

A multiclass and multi-residue method was optimized and validated for analysis of 19 pesticides of 16 chemical classes in greenhouse cucumber and tomato followed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). In this study a dispersive liquid-liquid microextraction (DLLME) technique was applied for extraction and pre-concentration of pesticide residues from QuEChERS (quick, easy, cheap, effective, rugged and safe) extracts. The DLLME method was carried out using carbon tetrachloride as the extraction solvent and QuEChERS extract as the dispersive solvent. The main parameters affecting DLLME efficiency including the type and volume of extraction solvent and the volume of dispersive solvent and salt addition were optimized for the proposed method. To validate this developed method, recovery studies were carried out at two concentration levels, yielding mean recovery rates in the range of 86 to 104% with relative standard deviations below 12%. Good linearity and precision, with relative standard deviations generally below 10%, were obtained for all 19 pesticides. The method limits of detection (LOD) between 3.4 and 10.4 μg kg-1 and method limits of quantification (LOQ) in the range of 11.2-34.5 μg kg-1 were obtained for the proposed method. Recovery rates and method LODs and LOQs of the validated method were compared with those of the routine QuEChERS method and results indicated the efficiency of the proposed method for routine analysis of 19 pesticide residues in cucumber and tomato. © 2013 The Royal Society of Chemistry.

Alipanah H.,Iranian Research Institute of Plant Protection IRIPP | Baixeras J.,University of Valencia
Zootaxa | Year: 2011

Hedya tritofa, new species, is described and illustrated based on eleven males and five females collected in northern Iran (Gilan, Mazandaran, and Golestan provinces). Morphology and diagnostic characters of Hedya Hübner, 1825, Metendothenia Diakonoff, 1973, the Olethreutes group of genera, and the Neopotamia group of genera are discussed. We propose the resurrection of the combinations Hedya atropunctana (Zetterstedt, 1840), revised status, and H. separatana (Kearfott, 1907), revised status, and the new combination H. inouei (Kawabe, 1987). Copyright © 2011 Magnolia Press.

Alipanah H.,Iranian Research Institute of Plant Protection IRIPP | Gielis C.,Mr. Haafkensstraat
SHILAP Revista de lepidopterologia | Year: 2010

Twenty-seven species of the two tribes Platyptiliini and Exelastini are listed. The genus Exelastis and the following species are reported from Iran for the first time: Platyptilia calodactyla ([Denis & Schiffermüller]), P. nemoralis Zeller, P. farfarellus Zeller, Gillmeria armeniaca (Zagulajev), G. pallidactyla (Haworth), G. ochrodactyla ([Denis & Schiffermüller]), Stenoptilia zophodactylus (Duponchel), S. lucasi Arenberger, Paraplatyptilia metzneri (Zeller), M. asiatica (Rebel) and Exelastis atomosa (Walsingham).

Ebrahimi E.,Iranian Research Institute of Plant Protection IRIPP | Carpenter J.M.,American Museum of Natural History
Zoology in the Middle East | Year: 2012

Two species of hornets are present in Iran: Vespa orientalis Linnaeus, 1771, has a widespread distribution in most parts of Iran, except for the Caspian coast in northern Iran, but V. crabro Linnaeus, 1758, is present only on the Caspian coast. The ambiguity regarding these two species in Iran, their distribution patterns, diagnoses and agricultural aspects are discussed. © Kasparek Verlag, Heidelberg.

Farzadfar S.,Iranian Research Institute of Plant Protection IRIPP | Pourrahim R.,Iranian Research Institute of Plant Protection IRIPP
Journal of Phytopathology | Year: 2014

Brassicaceae crops in eight provinces of the North-west Iran were surveyed for Turnip mosaic virus (TuMV) infection during 2011 and 2012. Many symptomatic plants (38%; 226 of 598) were found to be infected with TuMV. The highest frequency was in turnip (61%), followed by radish (55%), oilseed rape (38%), and brassica weeds including annual bastard cabbage (42%), small tumbleweed-mustard (50%) and wild radish (45%), but not Brassica oleracea and Lepidium sativum. Using biological assays, Iranian TuMV isolates grouped in three [B], [B(R)] and [BR] host-infecting types. Phylogenetic analysis using complete coat protein (CP) gene nucleotide sequences showed that the Iranian isolates belonged to the Basal-B and Asian-BR populations. No evidence of recombination was found in these isolates using different recombination-detecting programmes. To our knowledge, our study shows for the first time the occurrence of TuMV Asian-BR subpopulation in the mid Eurasian region of Iran. The data suggest that the Asian-BR subtype population is found across southern Eurasia and might be a continuous population in East Asia (mostly Japan and China) and Minor Asia (Turkey), the places considered to be one of the origins of TuMV populations. © 2014 Blackwell Verlag GmbH.

Pourrahim R.,Iranian Research Institute of Plant Protection IRIPP | Farzadfar S.,Iranian Research Institute of Plant Protection IRIPP
Journal of Phytopathology | Year: 2016

Samples of trumpet creeper (Campsis radicans) leaves showing mottling and mosaic were collected from plants growing in a private garden in Tehran province, Iran, in 2012. Symptomatic leaf samples were tested for Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV) and Peanut stunt virus (PSV) infection in enzyme-linked immunosorbent assay (ELISA), using specific antibodies. None of the samples were positive for CMV and PSV; however, all reacted positively with that of AMV antiserum. In biological assay, systemic infection was found on Datura stramonium, Nicotiana tabacum cvs., White Burley, and Xanthi, 21 days postinoculation (DPI), while necrotic local lesions were obtained following inoculation of Phaseolus vulgaris and Vigna unguiculata within three to four DPI. Using a pair of primers specific for AMV, a DNA fragment of 880 bp was RT-PCR-amplified. Analysis of the sequences revealed the presence of 657 nucleotides of AMV complete coat protein (CP) gene (translating 218 amino acid residues). Phylogenetic analysis using neighbour-joining (NJ) method clustered AMV isolates into two main types and the IRN-Tru (GenBank Accession No. JX865593) isolate fell into type I. Pairwise nucleotide distances also confirmed two main types with the highest and lowest similarities for type I and II, respectively. The association of AMV with mosaic disease of C. radicans represents the first record from the world. © 2016 Blackwell Verlag GmbH.

Pourrahim R.,Iranian Research Institute of Plant Protection IRIPP | Farzadfar S.,Iranian Research Institute of Plant Protection IRIPP
Journal of Phytopathology | Year: 2014

Alfalfa fields in three western provinces of Iran were surveyed for Peanut stunt virus (PSV) during 2011 and 2012. Forty-seven of 115 samples tested (41%) were infected with PSV. Phylogenetic analysis using coat protein (CP) gene sequences showed that the Iranian isolates belong to the subgroup II of PSV. Pairwise identity analysis revealed four groups representing four phylogenetic subgroups. PSV strains in subgroups III and IV are closely related to each other, as supported by the lowest nucleotide diversity, high pairwise nucleotide identity and high haplotype diversity as evidence of a recent population expansion after a genetic bottleneck. Using the maximum likelihood method, amino acid 86S in the CP gene of the Iranian PSV isolates was found to be under positive selection, although the likelihood ratio test statistics is not significant. This is the first report of the occurrence and phylogenetic relationships of Iranian PSV isolates in west Iran. © 2014 Blackwell Verlag GmbH.

Marzban R.,Iranian Research Institute of Plant Protection IRIPP
Journal of Biopesticides | Year: 2012

Bacillus thuringiensis bioinsecticide has been widely used on crops worldwide to replace chemical pesticides. B. thuringiensis production by solid-state fermentation requires less capital investment and modest technical skills. The method is often considered unsuitable for growth of aerobic organisms. However, optimization of Bt production using solid-state fermentation can effectively contribute to promote use of this bacterium in insect pest management programs. Research into suitable nutrient concentrations of different media and characteristics of bacterial growth on these has enabled use of several agricultural or industrial by-products for mass production of several Bt strains. These materials include wheat bran, rice bran, rice crumb, and remaining barley from feeding of Sitotroga serealella. Wheat bran was the best of media for production of B. thuringiensis. ©JBiopest.

Baniameri V.,Iranian Research Institute of Plant Protection IRIPP | Cheraghian A.,Iranian Research Institute of Plant Protection IRIPP
EPPO Bulletin | Year: 2012

In Iran, the tomato growing area is about 150 000 ha, mostly located in the south of the country. The Iranian Plant Protection Organization and inspection service in Iranian Research Institute of Plant Protection (IRIPP) prepared a monitoring program for this pest in 2009 and 2010. A technical guideline was issued and distributed among PPO inspectors. For the first time in November 2010, samples were collected by Uromiyeh PPO on tomato from Uromiyeh in Azarbaijan province in North West Iran. The specimens found in these samples were identified as Tuta absoluta. Three months later in January 2011, pheromone traps were placed in tomato growing areas in Borazjan, Busher province, to detect and monitor this pest. T. absoluta was identified, based on adult morphology and on male genitalia from moths collected in 5 of these traps during January 2011. More pheromone traps were put into place covering the entire tomato growing area throughout the country. As of June 2011, the pest was detected in 24 different locations. This is the first report of T. absoluta in Iran. T. absoluta is a very serious pest for tomato, and an outbreak of this pest is expected during the crop cycle in autumn and winter 2011-2012 in the south of Iran. Thus, based on experiences in other countries an IPM program according to available tools and materials was developed. © 2012 The Authors. Journal compilation © 2012 OEPP/EPPO.

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