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Mehdi R.M.,Islamic Azad University | Reza M.S.,Tabriz University of Medical Sciences | Mohammad R.,Iranian Health Reference Laboratory | Mohammad R.,Tehran University of Medical Sciences
HealthMED | Year: 2012

Background and objectives: Drug resistant tuberculosis (TB) is a man-made problem. While tuberculosis is hundred percent curable, multi drug resistant tuberculosis (MDR-TB) is difficult to treat. Inadequate and incomplete treatment and poor treatment adherence has led to a newer form of drug resistance known as extensively drug resistant tuberculosis (XDR-TB). The aim of this study was to determine prevalence of XDR-TB in east Azerbaijan province of Iran. Methods: Drug susceptibility testing to second line drugs including amikacin, kanamycin, ofloxacin and ciprofloxacin was performed on isolated eight MDR-TB strains. H37RV Mycobacterium tuberculosis (susceptible to all drugs) was used as a control strain. Results: Eight strains out of 230 isolates (3.48%) were diagnosed to be MDR-TB, and three strains (1.3%) out of eight MDR-TB strains, were found to be XDR. All three XDR strains were from patients who had come to Iran from Nakhchivan of Azerbaijan. All patents were male. Conclusion: Since the XDR-TB was only seen from Nakhchivanian patients isolates, referring these patients to Tabriz Research Center of TB and pulmonary diseases for diagnosis and treatment is problematic, and special measures should be taken. Source

Maham S.,Tehran University of Medical Sciences | Shirvani F.,Tehran University of Medical Sciences | Jahromi M.H.,Tehran University of Medical Sciences | Fallah F.,Tehran University of Medical Sciences | And 4 more authors.
Journal of Pure and Applied Microbiology | Year: 2012

Bloodstream infectious diseases are prevalent in children and adults. Rapid and accurate diagnosis of bloodstream infections primary based on conventional culture results, which saves time and prevents empiric treatment. The aim of this study was to compare the results of BACTEC 9120 and conventional culture systems, on blood specimens collected from three university affiliated hospitals in Tehran, Iran. BACTEC 9120 and conventional methods were used for isolation of microorganism from specimens including blood and other body fluid samples collected from patients hospitalized in the selected hospitals during the study period, from April to June 2009. Time for positive and negative results and hospital charge were estimated for the two culture methods. In total 747 specimens were possessed by BACTEC 9120 systems and 787 by conventional method. Patients aged between 3 days and 8 years old, (mean 11.4±21.9 years); 52% of patients were male and 48% female; Out of 747 specimens were possessed by BACTEC 9120 system, 26% (196/747) and from 787 specimens cultured by conventional method 5% (49/787) were positive (p<0.05). Hospital stay was 13.8 ± 12.9 days in BACTEC 9120 and 17.9 ± 14.9 days in Conventional method,(p<0.05) respectively. Time for positivity by BACTEC system was 3.8 ± 1.1 Days and 5.9 ± 2.5 days in conventional method.(p<0.05), treatment response showed 1.8 day earlier result in patients that their specimens were processed by BACTEC 9120 system. Death rate in BACTEC 9120 method was 6% and 11% in Conventional method. (p>0.05).In conclusion implementation of BACTEC system for microbiologic detection of pathogens decreases the admission time and early diagnosis and treatment results are cost effective for patient management and prevention of antibiotic resistance. Source

Marandi F.R.,Iranian Health Reference Laboratory | Rahbar M.,Iranian Health Reference Laboratory | Sabourian R.,Iranian Health Reference Laboratory | Saremi M.,Iranian Health Reference Laboratory
Journal of the Pakistan Medical Association | Year: 2010

Objective: To determine the ability of Iranian microbiology laboratories for identification and susceptibility testing of Streptococcus pneumoniae and Haemophilus influenzae as causative agents of bacterial meningitides. Methods: Two strains of bacteria including Haemophilus influenzae and Streptococcus pneumoniae as a common causative agents of meningitides were chosen and coded as strain number 1 and number 2. The strains were distributed among 679 microbiology laboratories. All laboratories were requested for identification of each unknown microorganism and susceptibility testing of S. pneumoniae against five commonly used antibiotics. Results: Of 679 microbiology laboratories 310 (46%) laboratories participated in the survey and among these, 258 laboratories completely identified S. pneumoniae. About 85% laboratories produced correct susceptibility testing against oxacillin, erythromycin, tetracycline, and vancomycin. Of 310 received responses only 50 laboratories identified H. influenza correctly. The majority of the laboratories did not have the capacity to identification H. influenza. Conclusion: Microbiology laboratories in our country are qualified for identification and susceptibility testing of S. pneumoniae. However, majority of laboratories are not qualified for identification of H. influenzae. Source

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