Iranian Fisheries science Research Institute

Bandar ‘Abbās, Iran

Iranian Fisheries science Research Institute

Bandar ‘Abbās, Iran
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Naderi M.,Khorramshahr Marine Science and Technology University | Keyvanshokooh S.,Khorramshahr Marine Science and Technology University | Salati A.P.,Khorramshahr Marine Science and Technology University | Ghaedi A.,Iranian Fisheries Science Research Institute
Aquaculture | Year: 2017

A 60 day feeding experiment was conducted to evaluate the effects of dietary vitamin E (vit E), selenium nanoparticles (nanoSe), and their combination on humoral immune status and serum parameters of rainbow trout under high density condition. A total of 1275 rainbow trout with an average weight of 42.6 ± 2.3 g were acclimatized, distributed into 15 tanks and divided into five experimental groups according to stocking density and diet: Normal control (20 kg m− 3; basal diet), Dense control (80 kg m− 3; basal diet), Vit E (80 kg m− 3; 500 mg kg− 1 vit E-supplemented diet), NanoSe (80 kg m− 3; 1 mg kg− 1 nanoSe-supplemented diet), and Combination (80 kg m− 3; 500 mg kg− 1 vit E and 1 mg kg− 1 nanoSe-supplemented diet). Each group consisted of three tanks. High stocking density reduced weigh gain, specific growth rate, feed intake, feed conversion ratio, serum agglutination antibody titer, total antioxidant capacity, and globulin level, but increased lysozyme, albumin, and cholesterol levels when basal diet was fed. However, vit E supplementation to diets significantly improved growth performance and health status of rainbow trout. While the combination of nanoSe with vit E promoted the performance of the fish, supplementation with nanoSe had no significant effects on performance in rainbow trout under high density conditions, suggesting that the beneficial effects observed in the performance of the Combination group may be due to vitamin E alone. © 2017 Elsevier B.V.


Memar B.,Islamic Azad University at Tehran | Jamili S.,Islamic Azad University at Tehran | Jamili S.,Iranian Fisheries Science Research Institute | Shahbazzadeh D.,Pasteur Institute of Iran | Bagheri K.P.,Pasteur Institute of Iran
Toxicon | Year: 2016

Pterois russelli is a venomous fish belonging to scorpionidae family. Regarding to high significance value for tracing potential therapeutic molecules and special agents from venomous marine creatures, the present study was aimed to characterization of the Persian Gulf lionfish venom. Proteolytic, phospholipase, hemolytic, coagulation, edematogenic and dermonecrotic activities were determined for extracted venom. The LD50 of P. russelli venom was determined by intravenous injection in white Balb/c mice. Phospholipase A2 activity was recorded at 20 μg of total venom. Coagulation activity on human plasma was shown by Prothrombin Time (PT) and activated Partial Thromboplastin Time (APTT) assays and coagulation visualized after 7 and 14 s respectively for 60 μg of crude venom. LD50 was calculated as 10.5 mg/kg. SDS-PAGE revealed the presence of major and minor protein bands between 6 and 205 kDa. Different amounts of crude venom ranged from 1.87 to 30 μg showed proteolytic activity on casein. The highest edematic activity was detected at 20 μg. Our findings showed that the edematic activity was dose dependent and persisted for 48 h after injection. The crude venom did not induce dermonecrotic activity on rabbit skin and showed no hemolytic activity on human, mouse and rabbit erythrocytes. This is the first report for phospholipase A2 and coagulation activity in venomous fish and venomous marine animals respectively. Proteolytic activity of P. russelli venom is in accordance with the other genara of scorpionidae family. According to venom activity on intrinsic and extrinsic coagulation pathways, lionfish venom would be contained an interesting pharmaceutical agent. This study is pending to further characterization of phospholipase A2, coagulation, and protease activities and also in vivo activity on animal model of surface and internal bleeding. © 2016 Elsevier Ltd. All rights reserved.


Mazandarani M.,Gorgan University of Agricultural Sciences and Natural Resources | Hoseini S.M.,Iranian Fisheries Science Research Institute
Aquaculture Research | Year: 2017

This study describes anaesthetic efficacy of menthol and 1,8-cineole in common carp, Cyprinus carpio, in comparison with eugenol. Common carp fingerlings were exposed to eugenol: 5, 10, 15, 25, 35, 50, 75, 100, 150 and 200 ppm; menthol: 5, 10, 15, 25, 50, 100, 200, 300, 400, 500 and 600 ppm; 1,8-cineole: 100, 150, 200, 300, 400, 500, 600, 700 and 800 ppm. Induction time and recovery time were recorded. Results showed that menthol and 1,8-cineole anesthetized the fish at higher concentrations compared to eugenol. The fish exposed to menthol showed common fish behavioural responses to anaesthesia, similar to eugenol. But, 1,8-cineole-exposed fish showed tail-up swimming, which was not observed before. Also, 1,8-cineole failed to completely cease muscle tone. Exposure to 200 ppm eugenol and 600 ppm menthol resulted in 40% and 20% mortality, respectively. Induction time was exponentially dependent on anesthetic concentrations. Recovery time was linearly correlated to eugenol and menthol, but not 1,8-cineole concentrations. Recovery time was exponentially dependent on induction time in the fish anesthetized with eugenol and menthol, but not 1,8-cineole. Menthol and 1,8-cineole are recommended for carp anaesthesia. Menthol is capable to anesthetize common carp within 1–3 min at 118–512 ppm. Common carp anesthetized with 108–133 ppm menthol recovers within 5 min. 1,8-cineole failed to anesthetize common carp within less than 150 s at 300–800 ppm concentrations. However, it anesthetizes carp within 3 min at 595 ppm concentration. Also, 1,8-cineole is not recommended for fish surgery. Both menthol and 1,8-cineole were less efficacious than eugenol. © 2016 John Wiley & Sons Ltd


Hajizadeh A.,Iranian Fisheries Science Research Institute | Shinn A.,University of Stirling
Iranian Journal of Fisheries Sciences | Year: 2016

This study investigates the effects of dietary lipid sources on the growth and reproductive performance of Nile tilapia over three consecutive spawnings. Fish were reared using three experimental diets, with the goal of replacing dietary fish oil with palm oil. Three experimental diets and a commercial trout diet was used as the control. The effect of dietary lipid on the growth performance, spawning interval, fecundity, relative fecundity (number of eggs per unit weight), egg size, egg fertilization and hatching rate in addition to an assessment of larval quality was investigated. Growth was significantly (p<0.05) influenced by the source of dietary lipid used. The source of the dietary lipid, however, had no significant effect on the diameter of the eggs, as well as their volume or dry weight. Despite this, relative fecundity was found to be significantly different between fish fed experimental diets and those fed the control diet; there was no difference between those fed the PO and mixed PO:CO diets (p>0.05). Similar results were observed for the egg to body weight ratio (EW: BW) and the inter-spawning interval (ISI) for the fish fed control diet (diet 4). The total fecundity (number of eggs produced per fish) obtained from the fish fed the mixed oil diet (PO:CO) was significantly higher (p<0.05) than from those fed the palm oil and control diets. This study suggests that palm oil can replace fish oil in diets fed to O. niloticus with no subsequent negative effects on the eggs and larval quality.


Jafarpour M.,Islamic Azad University at Urmia | Fard A.N.,Iranian Fisheries Science Research Institute
AACL Bioflux | Year: 2016

The lemon balm (Melissa officinalis) has long been considered as traditional medicine which is used as an analgesic and anti-microbial plant. The aim of this study was to investigate the effects of aqueous extract of M. officinalis on liver enzyme alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and total protein and to study its pathology effect on Oncorhynchus mykiss liver. For this study, fishes with an mean weight of 85±5 g in 3 groups were examined which includes: control (without extract); test group 1 (450 mg per kg of body weight aqueous extract of M. officinalis); and test group 2 (1,350 mg per kg of body weight aqueous extract of M. officinalis), each with a 2 repetition treatments daily for 30 days, feeding with extracts and concentrates were in extruded form. Growing conditions (water temperature 14°C, the amount of oxygen dissolved in water of 9 mg/mL) was similar for all groups. The results of blood at the end of the 30 day showed a significant reduction of ALT and AST in blood serum in test groups 1 and 2 against control (p>0.05). Comparison of the total protein in blood serum test for all groups indicated significant differences (p>0.05). During comparative pathology examination of the liver, the waste is observed in the test group, compared to the control group. © 2016, BIOFLUX SRL. All rights reserved.


Kakoolaki S.,Iranian Fisheries Science Research Institute | Afsharnasab M.,Iranian Fisheries Science Research Institute
Crustaceana | Year: 2017

The White Spot Syndrome Virus has already posed a serious threat to industrial shrimp culture for many years. We studied this disease in Penaeus vannamei. A total of 100 specimens was used to evaluate tissue tropism forWSSV. In case of infectious correlations among the tissues, Kendall's taub test showed that there were significant differences (P<0.05) among the target tissues. The rates of frequency for infected target tissues, which should be calculated between 0 and 1, were 0.71, 0.58, 0.61 and 0.12, respectively, for haemolymph, hindgut epithelial cells, cephalothoracic epidermis, and interstitial tissue of the hepatopancreas. Results indicated that 88% of the gills, 98% of the muscles, 97% of haematopoietic nodules and 97% of the hearts were not severely infected, but these rather showed relatively mild infections. It can be suggested that epithelial cells of the gills prevent replication of the virus in the early stage of WSSV infection, whereas meanwhile epithelial cells of the cephalothorax and those of the hindgut, in contrast, boost the replication ofWSSV. Additionally, morphological haemocytic evaluation can be applied as an appropriate infectious indicator in the early stage of WSSV disease in farmed P. vannamei. © 2017 Koninklijke Brill NV, Leiden.


Sh D.,Iranian Fisheries Science Research Institute
Iranian Journal of Fisheries Sciences | Year: 2016

The effects of n-3 highly unsaturated fatty acids (n-3 HUFA) were studied on Beluga (Huso huso) fish larvae by feeding fish larvae with live food enriched with 4 different oils (ICES 30/4, tuna eye oil, flaxseed oil and linseed oil) containing different levels of n-3 HUFA including 27.19, 20.33, 12.71 and 0.39 mg g-1 dry weight of oil, respectively. Fish larvae weighing 30.00±2.00 mg were randomly divided into 12 groups of 150 fish each and triplicates fed the 4 experimental diets for 3 weeks. There were no differences in survival rates, but significant differences were found in growth rates and fatty acid composition of the fish larvae after 30 days. Fish fed low levels of dietary n-3 HUFA (0.39 mg g-1 DW) showed poor n-3 HUFA contents but these fatty acids improved with the elevation of the n-3 HUFA levels in the diet up to 20 mg g-1 DW. Fish fed the flaxseed oil showed the highest level of crude lipid 15.00±3.01). Results showed that tuna eye oil and ICES30/4-enriched live food (4.83 ±1.29 and 4.61 ±0.99 mg g-1 DW, respectively) showed the highest n-3 HUFA content for sturgeon fish larvae.


Taheri Mirghaed A.,University of Tehran | Ghelichpour M.,University of Tehran | Hoseini S.M.,Iranian Fisheries Science Research Institute
Aquaculture | Year: 2016

The aim of this study was to investigate anesthetic efficacy of myrcene and linalool on Cyprinus carpio in comparison with eugenol. The fish were exposed to 150, 200, 400, 600, 800, 1000 and 1200 ppm myrcene, 200, 400, 600, 800, 1000, 1200, 1400 and 1600 ppm linalool, and 25, 35, 50, 75, 100, 150 and 200 ppm eugenol and induction time were recorded based on fish behaviors. Exposure of the fish to 150-1000 ppm myrcene led to 594-42.7 s induction time with no significant difference between 1000 and 1200 ppm. Recovery at 150-800 ppm myrcene occurred within 149-272 s with no significant difference among 800-1200 ppm. Exposure to 200-1400 ppm linalool resulted in 812-79 s induction time, with no significant difference between 1400 and 1600 ppm. There was no significant difference in recovery time at different linalool concentrations (150-165 s). Induction time in both anesthetics were significantly dependent on anesthetic concentrations (R2 = 0.94-0.96). Recovery time in the myrcene-exposed fish was dependent on anesthetic concentrations (R2 = 0.77). Using regression models, calculated myrcene concentrations to induce stage 4 anesthesia within 60 and 180 s were 819 and 350 ppm. Required linalool concentration to induce stage 4 anesthesia within 180 s was calculated to be 753 ppm. Myrcene at 50 ppm and linalool at 50 and 100 ppm are able to keep the fish at stage 2 anesthesia for 2 h. This study showed for the first time that linalool and myrcene are potential anesthetic in carp, although not as efficacious as eugenol. Statement of relevance: Anesthetics are used in many aquaculture-related activities. Herbal materials have been considered as alternatives for chemical anesthetics in fish due to their potential health benefits. Myrcene is not studied as anesthetic in fish. Limited data are available on linalool anesthetic efficacy in fish. Therefore, we conducted this study to investigate their potential anesthetic efficacy in carp. © 2016 Elsevier B.V.


Afsharnasab M.,Iranian Fisheries Science Research Institute | Kakoolaki S.,Iranian Fisheries Science Research Institute | Mohammadidost M.,South Iranian Aquaculture Center
Fish and Shellfish Immunology | Year: 2016

This paper investigates the efficacy of Gracilaria corticata, Saccharomyces cerevisiae and gamma irradiation WSSV as immunostimulants to white shrimp Litopenaeus vannamei. Seven hundred and twenty healthy shrimp SPF L. vannamei subadult with average weight of 10 ± 1.02 g were collected and divided into 8 groups. The first group (T1) was fed with commercial pellet, the second group (T2) fed with S. cerevisiae (2 g/kg), the third group (T3) fed with G. corticata powder mixed with shrimp feed (2 g/kg) and, finally, the fourth group (T4) was fed with commercial pellet and injected intramuscularly gamma irradiant WSSV (1 μl/gbw) for 10 days. The shrimps were then injected with WSSV and maintained for 25 days. The positive control group for each treatment was maintained in the same manner but without injection with WSSV. Moreover, survival rate and immune parameters such as total hemocyte count (THC), total protein plasma (TPP), superoxide dismutase (SOD) activity, peroxidase (POD) activity and phenoloxidase activity (PO) were determined. Results indicated that the survival rates for groups T4, T3 T2 and T1 were 57.05 ± 3.52%, 22.5 ± 0.5%, 15 ± 1.05% and 00.0 ± 0%, respectively. Ultimately, at the end of the study the shrimp group T4 showed higher hematological data: THC, TPP, SOD, POD and PO. The study concluded that gamma irradiant WSSV is effective immunostimulants in shrimp L. vannamei and the immunity has better performances than those of the G. corticata and S. cerevisiae. © 2016 Elsevier Ltd


Hosseini S.A.,Iranian Fisheries Science Research Institute | Kaymaram F.,Iranian Fisheries Science Research Institute
Journal of Applied Ichthyology | Year: 2016

In this paper, the examination of some reproductive features and stomach contents of yellowfin tuna, Thunnus albacares, were determined by samples taken from Iranian drifting gillnets in the Oman Sea in the years 2007 to 2009. The male to female ratio in overall size was 1: 0.93, which was not significantly different from the expected value 1: 1. Fish lengths ranged from 37 to 152 cm, averaging 78.5 cm for females and 79.2 cm for males. At a larger size (>117 cm) males were proportionally predominant. Length at first maturity, Lm 50%, was observed when females reached 77.2 cm. Data from maturity stages indicated a single spawning period, peaking in May-June, corresponding with a drop in the Gonadosomatic Index (GSI). Fishes were the main prey group, comprising 47.6% of total numbers among the three food item categories in the tuna stomachs. Among the 12 families identified in the stomachs, the Portunidae swimming crab crustacean was the most important prey organism (18.7% of total numbers). A wide range of prey species found in yellowfin tuna stomachs reflects an opportunistic feeding behaviour restricted by local prey availability. Considering the scarcity of available data, the results of the present paper will provide a reference for better knowledge of the biological features of T. albacares in the Oman Sea. © 2016 Blackwell Verlag GmbH.

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