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Hoseinifar S.H.,University of Tehran | Mirvaghefi A.,University of Tehran | Mojazi Amiri B.,University of Tehran | Rostami H.K.,Iranian Fisheries Research Organization IFRO | Merrifield D.L.,University of Plymouth
Aquaculture Nutrition

Preliminary experiments were undertaken to investigate the effects of oligofructose on beluga sturgeon (Huso huso) growth performance, survival and culturable autochthonous intestinal microbiota. Juveniles (20g) were fed diets containing varying levels of oligofructose (10, 20 and 30gkg-1) at 2-3% body weight per day for 7weeks. Compared to the control group, no significant (P>0.05) effect on growth performance was observed in fish fed diets supplemented with oligofructose at 10 and 20gkg-1. However, compared to the 20gkg-1 group, feeding oligofructose at 30gkg-1 resulted in adverse effects on growth performance. Dietary supplementation of oligofructose at 20gkg-1 significantly increased survival rate. Microbiological assessment indicated that the viable culturable autochthonous levels were not affected by dietary oligofructose. Although lactic acid bacteria (LAB) were not a dominant component of the endogenous autochthonous microbiota, LAB levels were significantly elevated in fish fed 20gkg-1 dietary oligofructose. This elevated LAB population was able to persist for at least 1week after reverting the prebiotic group back to a control diet. This study encourages further research on different aspects of oligofructose in sturgeon culture with clear emphasis on optimizing dosage levels. © 2011 Blackwell Publishing Ltd. Source

Hoseinifar S.H.,Gorgan University of Agricultural Sciences and Natural Resources | Khalili M.,Islamic Azad University | Khoshbavar Rostami H.,Iranian Fisheries Research Organization IFRO | Esteban M.T.,University of Murcia
Fish and Shellfish Immunology

The aim of this study was to assess the effects of galactooligosaccharide (GOS), on the growth performance, stress resistance and intestinal microbiota of Caspian roach ( Rutilus rutilus) fry. Specimens (1.36±0.03g) were fed either a basal control diet (0% GOS, non-supplemented) or the basal diet supplemented with 1% and 2% of GOS. After 7 weeks of the feeding trials, growth factors (final weight, weight gain, specific growth rate, condition factor and food conversion ratio) as well as body composition, resistance to salinity stress and autochthonous intestinal microbiota were assessed. Results demonstrated that at the end of the trial growth factors (final weight, weight gain, SGR, FCR) were significantly higher in 2% GOS fed fish ( P<0.05). In addition, supplementation of GOS significantly increased both survival rate ( P<0.05), and resistance to a salinity stress challenge of prebiotic fed groups ( P<0.05). However, body composition and the total autochthonous intestinal heterotrophic bacteria counts remained unaffected in different treatments ( P>0.05). Nevertheless, autochthonous lactic acid bacteria levels were significantly elevated in fish fed 2% dietary GOS ( P<0.05). These results confirm that GOS improves growth performance, stress resistance and modulates intestinal microbiota by increasing lactic acid bacteria of Caspian roach fry, a very important fish species in the Caspian Sea. © 2013 Elsevier Ltd. Source

Sadighzadeh Z.,Islamic Azad University at Tehran | Tuset V.M.,CSIC - Institute of Marine Sciences | Valinassab T.,Iranian Fisheries Research Organization IFRO | Dadpour M.R.,University of Tabriz | Lombarte A.,CSIC - Institute of Marine Sciences
Marine Biology Research

The anatomical and morphometric (shape indices, contour descriptors and otolith weight) characterizations of sagittal otoliths were investigated in 11 species of Lutjanus spp. inhabiting the Persian Gulf. This is the first study that compares the efficiency of three different image analysis techniques for discriminating species based on the shape of the outer otolith contour, including elliptical Fourier descriptors (EFD), fast Fourier transform (FFT) and wavelet transform (WT). Sagittal otoliths of snappers are morphologically similar with some small specific variations. The use of otolith contour based on wavelets (WT) provided the best results in comparison with the two other methods based on Fourier descriptors, but only the combination of the all three methods (EFD, FFT and WT) was useful to obtain a robust classification of species. The species prediction improved when otolith weight was included. In relation to the shape indices, only the aspect ratio provided a clear grouping of species. © 2012 Copyright Taylor and Francis Group, LLC. Source

Hoseinifar S.H.,University of Tehran | Mirvaghefi A.,University of Tehran | Merrifield D.L.,University of Plymouth | Amiri B.M.,University of Tehran | And 2 more authors.
Fish Physiology and Biochemistry

A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets, haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P>0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly higher (P>0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and 3% oligofructose group (P<0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles could be affected by prebiotics, which should be taken into account in future studies. © 2010 Springer Science+Business Media B.V. Source

Katuli K.K.,University of Tehran | Amiri B.M.,University of Tehran | Massarsky A.,University of Ottawa | Yelghi S.,Iranian Fisheries Research Organization IFRO

The stocks of Caspian roach (Rutilus rutilus), an economically important species in the Caspian Sea, are depleting. Each year millions of artificially produced fingerlings of this species are restocked in the mouth of rivers of the Southern Caspian Sea (e.g. Qare Soo River), where they are exposed to pesticides originating from regional rice and orchard fields. This early exposure to pesticides could affect the hypo-osmoregulatory ability of juvenile fish. Thus, in this study, Caspian roach fingerlings were exposed to environmentally-relevant concentrations of the organophosphate insecticide diazinon for 96h in fresh water and then transferred to diazinon-free brackish water (BW) for another 96h. We report that cortisol and glucose levels were significantly increased in all diazinon treatments at all sampling time points in comparison to the control group. Moreover, the thyroid hormone levels of TSH, T4, and T3 significantly decreased in diazinon-exposed fish even after the transfer to BW. The electrolytes were differentially affected during the exposure to diazinon and after the transfer to BW. The number of chloride cells in the gill tissue was significantly increased during diazinon exposure at the higher concentrations and decreased to control levels after transfer to BW. Finally, gill and kidney tissues showed many histopathological changes in diazinon-exposed fish even after 240h in BW. These results suggest that the release of Caspian roach fingerlings into the diazinon-contaminated Caspian Sea regions may alter their physiology and jeopardize their survival, which could lead to a failure in rebuilding the Caspian roach stocks in the Caspian Sea. © 2014 Elsevier Ltd. Source

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