PubMed | Tehran University of Medical Sciences, Zanjan University of Medical Sciences, Pasteur Institute of Iran and Iranian Blood Transfusion Organization
Type: Journal Article | Journal: Iranian journal of parasitology | Year: 2017
Aurones are naturally occurring compounds that belong to flavenoids family and have antiplasmodial effects. This study investigated some new aurones derivatives against chloroquine sensitive The cytotoxic evaluations of active compounds were performed with MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide) assay on human breast cancer cell lines; MCF7 and T47D.From 11 compounds M3, M6 and M7 compounds showed good anti-plasmodial effect against chloroquine-sensitive 3D strain of The replacement of the 4 and 5 positions at ring B of aurone derivatives, with propoxy and bromide (Br) respectively was revealed highly advantageous for their antiplasmodial effect.
Hakeshzadeh F.,Shahid Beheshti University of Medical Sciences |
Tabibi H.,Shahid Beheshti University of Medical Sciences |
Ahmadinejad M.,Iranian Blood Transfusion Organization |
Malakoutian T.,Tehran University of Medical Sciences |
Hedayati M.,Shahid Beheshti University of Medical Sciences
Renal Failure | Year: 2010
Background: Hypercoagulability is an important risk factor for thrombosis and its complications in hemodialysis patients. This study was designed to investigate the effects of l-carnitine supplement on plasma coagulation and anticoagulation factors in hemodialysis patients. Methods: Thirty-six hemodialysis patients were randomly assigned to either a carnitine or a placebo group. Patients in the carnitine group received 1000 mgday oral l-carnitine for 12 weeks, whereas patients in the placebo group received a corresponding placebo. At baseline and the end of week 12, 5 mL blood was collected after a 12- to 14-hour fast and plasma fibrinogen concentration, activity of plasma protein C, coagulation factors V, VII, IX, and serum concentrations of tissue plasminogen activator (tPA), plasminogen activator inhibitor type-1 (PAI-1), free carnitine, and C-reactive protein (CRP) were measured. Results: In the carnitine group, mean serum free carnitine concentration increased significantly to 150% of baseline (p < 0.001), whereas plasma fibrinogen and serum CRP had 98 mg/dL (p < 0.01) and 41% (p < 0.01) significant decreases, respectively, at the end of week 12 compared with baseline. The reductions were significant compared with the placebo group (p < 0.05). No significant differences were observed between the two groups with regard to mean changes of the activity of plasma protein C, coagulation factors V, VII, IX, and serum PAI-1 to tPA ratio. Conclusion: l-Carnitine supplement reduces serum CRP, a marker of systemic inflammation, and plasma fibrinogen, an inflammation-related coagulation factor, in hemodialysis patients. Therefore, l-carnitine may play an effective role in preventing cardiovascular diseases in these patients. © 2010 Informa UK Ltd.
Enhanced detection sensitivity of prostate-specific antigen via PSA-conjugated gold nanoparticles based on localized surface plasmon resonance: GNP-coated anti-PSA/LSPR as a novel approach for the identification of prostate anomalies
PubMed | Iranian Blood Transfusion Organization, Mashhad University of Medical Sciences, Tehran University of Medical Sciences and Brighton & Sussex Medical School
Type: Journal Article | Journal: Cancer gene therapy | Year: 2016
Prostate-specific antigen (PSA) is used to screen for prostate disease, although it has several limitations in its application as an organ-specific or cancer-specific marker. Furthermore, a highly specific/sensitive and/or label-free identification of PSA still remains a challenge in the diagnosis of prostate anomalies. We aimed to develop a gold nanoparticle (GNP)-conjugated anti-PSA antibody-based localized surface plasmon resonance (LSPR) as a novel approach to detect prostatic disease. A total of 25nm colloidal gold particles were prepared followed by conjugation with anti-PSA pAb (GNPs-PSA pAb). LSPR was used to monitor the absorption changes of the aggregation of the particles. The size, shape and stability of the GNP-anti-PSA were evaluated by dynamic light scattering transmission electron microscopy (TEM) and zetasizer. The GNPs-conjugated PSA-pAb was successfully synthesized and subsequently characterized using ultraviolet absorption spectroscopy and TEM to determine the size distribution, crystallinity and stability of the particles (for example, stability of GNP: 443mV). To increase the stability of the particles, we pegylated GNPs using an N-(3-dimethylaminopropyl)-N*-ethylcarbodiimide hydrochloride (EDC)/N-hydroxylsuccinimide (NHS) linker (for example, stability of GNP after pegylation: 272mV). We found a significant increase in the absorbance and intensity of the particles with extinction peak at 545/2nm, which was shifted by ~1nm after conjugation. To illustrate the potential of the GNPs-PSA pAb to bind specifically to PSA, LSPR was used. We found that the extinction peak shifted 3nm for a solution of 100nM unlabeled antigen. In summary, we have established a novel approach for improving the efficacy/sensitivity of PSA in the assessment of prostate disease, supporting further investigation on the diagnostic value of GNP-conjugated anti-PSA/LSPR for the detection of prostate cancer.
Pourfathollah A.A.,High Institute for Research and Education on Transfusion Medicine |
Pourfathollah A.A.,Tarbiat Modares University |
Hosseini Divkolaye N.S.,Iranian Blood Transfusion Organization |
Seighali F.,High Institute for Research and Education on Transfusion Medicine
Transfusion Medicine | Year: 2015
Objective: This study aims to compare the changes and progress made to Iranian Blood Transfusion Organization (IBTO) during 1974-2014 in order to identify the shortcomings. Background: The History of Blood transfusion in Iran can be traced back to the 1940s. IBTO was established in 1974 as a national centralised organisation, supported by Iranian government for its budget and supplies and provides its products free of charge to both public and private hospitals. Methods: The statistics have been derived from IBTO Statistical Center. Also related literature has been reviewed. Results: From 1974 to 2014, donation per population has been increased about eight times. IBTO reached 100% voluntary blood donation in 2007, but the number of female blood donors in Iran is six times lower than average global rate. On one hand, the prevalence rate of Hepatitis B virus (HBV) and Hepatitis C virus (HCV) in blood donors was dropped in to one fifth and one third, respectively during past 8 years. On the other hand, irrational blood usage and lack of integrated blood stock management systems and non self-sufficiency on plasma-derivedmedicines are considered as main challenges of IBTO. Conclusion: Forty years since the establishment, IBTO managed to achieve considerable improvements in different fields but many challenges still remain, which need to be addressed urgently. Great gap between the number of male and female blood donors in Iran has to be filled. The improvement of knowledge and practice on patient blood management and use of alternatives are on the agenda of IBTO in next coming years. © 2015 British Blood Transfusion Society.
Zare-Zardini H.,Islamic Azad University at Yazd |
Zare-Zardini H.,Shahid Sadoughi University of Medical Sciences and Health Services |
Tolueinia B.,University of Sistan and Baluchestan |
Hashemi A.,Shahid Sadoughi University of Medical Sciences and Health Services |
And 2 more authors.
American Journal of Alzheimer's Disease and other Dementias | Year: 2013
Antioxidant agents and cholinesterase inhibitors are the foremost drugs for the treatment of Alzheimer's disease (AD). In this study, a new peptide from Ziziphus jujuba fruits was investigated for its inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes as well as antioxidant activity. This peptide was introduced as a new peptide and named Snakin-Z. The Snakin-Z displayed considerable cholinesterase inhibition against AChE and BChE. The half maximal inhibitory concentration (IC50) values of Snakin-Z against AChE and BChE are 0.58 ± 0.08 and 0.72 ± 0.085 mg/mL, respectively. This peptide has 80% enzyme inhibitory activity on AChE and BChE at 1.5 mg/mL. The Snakin-Z also had the high antioxidant activity (IC50 = 0.75 ± 0.09 mg/mL). Thus, it is suggested that Snakin-Z may be beneficial in the treatment of AD. However, more detailed researches are still required as in vivo testing its anticholinesterase and antioxidant activities. © The Author(s) 2013.
Zardini H.Z.,Shahid Sadoughi University of Medical Sciences and Health Services |
Zardini H.Z.,Islamic Azad University at Yazd |
Davarpanah M.,Ferdowsi University of Mashhad |
Shanbedi M.,Ferdowsi University of Mashhad |
And 3 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2014
In the present study, antimicrobial activities of multiwalled carbon nanotubes (MWCNTs) functionalized with ethanolamine (EA) groups were investigated. Therefore, MWCNT were first functionalized with mono-, di-, and triethanolamine (MEA, DEA, and TEA) under microwave technique. Development of functional groups on the MWCNT surface was confirmed by Fourier transform infrared and thermogravimetric analysis. Morphological variation was investigated by transmission electron microscopy. Then, antimicrobial activities of pristine and functionalized MWCNT (MWCNT-MEA, -DEA, and -TEA) were tested against different bacteria species. The studies have been done on four Gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, Pseudomonas aeroginosa, and Salmonella typhimurium) as well as four Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Bacillus cereus, and Streptococcus pneumonia). The results based on minimal inhibitory concentration and radial diffusion assay were shown that the antimicrobial activity of MWCNT-TEA > MWCNT-DEA > MWCNT-MEA > pristine MWCNT. Based on the results, it seems that EA groups could play an important role in antimicrobial activity of MWCNT. © 2013 Society of Plastics Engineers.
PubMed | Iranian Blood Transfusion Organization and Tehran University of Medical Sciences
Type: Journal Article | Journal: International journal of dermatology | Year: 2016
Thalassemia is a heterogeneous group of congenital hemoglobinopathies caused by mutations in the globin gene complex that result in an unbalanced globin synthesis. Unmatched globin chains bind to the cytosolic surface of red blood cell membrane where they cause oxidative damage that might in part be responsible for membrane weakness. The deformability of red blood cells and hypercoagulable state in thalassemic patients have been incriminated in leg ulcer formation, as this might cause ischemia to the skin and consequently friability and ulceration.Platelet-rich plasma (PRP) gel is considered an advanced wound therapy for chronic and acute wounds. PRP gel consists of cytokines, growth factors, chemokines, and a fibrin scaffold derived from a patients blood. In this study, we treated 100 thalassemic leg wounds using PRP.There was wound size reduction in patients after 4 weeks of treatment. In wounds with 2-3.5cmThis study will help extrapolation of the use of PRP for at least thalassemic leg wound treatment.
PubMed | Iranian Blood Transfusion Organization and Tehran University of Medical Sciences
Type: | Journal: Epidemiology and health | Year: 2016
Health promoting lifestyle (HPL) is one of the factors affecting the quality of life (QoL) among patients with beta-thalassemia (-thalassemia). Due to the lack of studies in this field, this study aimed at determining the association between HPL and QoL among adults with -thalassemia .This cross-sectional (descriptive-analytic )study conducted among 389 adult patients with -thalassemia in Tehran, Iran. The research instrument included a questionnaire consisting of three parts; demographic items, Short-Form Health Survey (SF-12) and Health Promoting Lifestyle Profile (HPLP-II). Data was analyzed by SPSS software version 23. Results were considered significant at the conventional p < 0.05 level.Mean age of participants was (30.238.3) years. The mean scores of HPL dimensions were (127.2821.53) and mean scores of QoL domains were (61.44 23.38). The highest and the lowest mean scores of HPL dimensions were found for spiritual growth (23.965.74) and physical activity (11.323.95) respectively. The scores of QoL in all three domains(total, physical component summary score, and mental component summary) were moderate .Health responsibility, physical activity, spiritual growth, and interpersonal relations were significant predicting factors of QoL in the adults with -thalassemia; these four dimensions explained 37.9% of the variance of QoL.QoL and HPL were not at acceptable levels among patients with thalassemia. Therefore, conducting educational interventions emphasizing on spiritual growth, physical activity, and interpersonal relations for patients with thalassemia are necessary.
PubMed | Isfahan University of Medical Sciences, Alborz University of Medical science, Tehran University of Medical Sciences and Iranian Blood Transfusion Organization
Type: | Journal: Clinical immunology (Orlando, Fla.) | Year: 2016
Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infections. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA and protein expression of PI3K, Akt and FoxO using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (pAkt) expression in B-cells has been measured by flowcytometry. We identified a significant reduction in the percentage of marginal zone like B-cells, memory B-cells (total, switched and unswitched) and plasmablasts in patients, as these decreased B-cell subsets had a significant negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased pAkt expression in B-cells of patients than HDs. We described for the first time impaired pAkt expression in B-cells of CVID patients that had a significant correlation with antibody response to the vaccine and worse clinical complications.
Garmiri P.,University of Cambridge |
Rezvan H.,Iranian Blood Transfusion Organization |
Abolghasemi H.,Iranian Blood Transfusion Organization |
Allain J.-P.,University of Cambridge
Journal of Medical Virology | Year: 2011
Iran is a low to medium endemic country for hepatitis B virus (HBV), depending on the region, where genotype D is dominant. Samples from 170 asymptomatic HBsAg-positive blood donors were quantified and the median viral load was 6.7×10 2IU/ml with 10.6% samples unquantifiable. Fifty complete genome sequences of these strains were characterized. Phylogenetic analysis identified 98% strains as subgenotype D1 and 2% as D2. Deduced serotypes were ayw2 (94%), ayw1 (4%), and adw (2%). The nucleotide diversity of the complete genome subgenotype D1 Iranian strains was limited (2.8%) and comparison with D1 strains from Egypt and Tunisia revealed little variation between strains from these three countries (range 1.9-2.8%). The molecular analysis of the individual genes revealed that the G1896A mutation was present in 86.2% of the strains and in 26 strains (29.9%) this mutation was accompanied by the G1899A mutation. The double mutations A1762T/G1764A and G1764T/C1766G were found in 20.7% and 24.1% of the strains, respectively. The pre-C initiation codon was mutated in five strains (5.8%). One strain had a 2-amino acid (aa) insertion at position s111 and another sP120Q substitution suggesting a vaccine escape mutant. © 2011 Wiley-Liss, Inc.