Iranian Biological Resource Center

Tehrān, Iran

Iranian Biological Resource Center

Tehrān, Iran
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Khansha J.,University of Tehran | Khansha J.,Iranian Biological Resource Center | Ranjbaran M.,University of Tehran | Amoozegar M.A.,University of Tehran
Geomicrobiology Journal | Year: 2017

Badab-e Surt spring is a travertine spring that has low to moderate levels of salt, so it is a good model for isolating moderately halophilic bacteria and investigating the relationship between microbe and environment. For isolating bacterial strains, water and sediment samples were collected from different springheads of the Badab-e Surt spring. Among the 171 bacterial isolates, 110 strains were halophiles. According to comparative partial 16S rRNA sequence analysis, the selected halophilic gram-positive and gram-negative strains were identified as members of the genera: Roseovarius, Labrenzia, Erythrobacter, Erythromicrobium, Massilia, Marinobacter, Halomonas, Shewanella, Pseudomonas, Flavobacterium, Bacillus, Brevibacterium, Staphylococcus, Microbacterium, Kocuria, and Streptomyces. To investigate mineralization, potential strains were screened by the culturing method, and then analyzed with a polarizing and scanning microscope. Five strains, Bss-11a, Bss-3, Bsw-1c1, Bsw-28d, and Bsw-39b, had potential for the mineralization of calcite that very closely resembled species Bacillus cohenii DSM 6307T, Labrenzia aggregate IAM 12614T, Bacillus safensis FO-036bT, Marinobacter flavimaris SW-145T, and Marinobater adhaerens HP15T, respectively. © 2017 Taylor & Francis Group, LLC


Makhdoumi-Kakhki A.,University of Tehran | Amoozegar M.A.,University of Tehran | Amoozegar M.A.,Iranian Biological Resource Center | Bagheri M.,Iranian Biological Resource Center | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

Strain EB21T was isolated from a brine sample from Aran-Bidgol salt lake, a saline playa in Iran. Strain EB21T was an orange-red-pigmented, motile rod and required at least 2 M NaCl but not MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C, while it was able to grow at pH 6.0-8.0 and 25-55 °C. Analysis of the 16S rRNA gene sequence revealed that strain EB21T is a member of the family Halobacteriaceae, showing low levels of similarity to other members of the family. The highest sequence similarities, 91.8, 91.7 and 91.5 %, were obtained with the 16S rRNA gene sequences of the type strains of Halobiforma lacisalsi, Haloterrigena thermotolerans and Halalkalicoccus tibetensis, respectively. Polar lipid analyses revealed that strain EB21T contains phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Three unidentified glycolipids and one minor phospholipid were also observed. The only quinone present was MK-8(II-H2). The G+C content of its DNA was 67.7 mol%. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain EB21T is thus considered to represent a novel species in a new genus within the family Halobacteriaceae, order Halobacteriales, for which the name Haloarchaeobius iranensis gen. nov., sp. nov. is proposed. The type strain of Haloarchaeobius iranensis is EB21T (=IBRC-M 10013T =KCTC 4048T). © 2012 IUMS.


Bagheri M.,Iranian Biological Resource Center | Bagheri M.,University of Tehran | Didari M.,Iranian Biological Resource Center | Amoozegar M.A.,University of Tehran | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A Gram-positive, moderately halophilic rod, designated X5BT, was isolated from saline mud of the hypersaline lake Aran-Bidgol in Iran. Strain X5BTwas a strictly aerobic, motile bacterium that produced ellipsoidal endospores at a central-subterminal position in non-swollen sporangia. The isolate grew at pH 7.0-10.0 (optimum pH 7.5), at 25-45 °C (optimum 35 °C) and with 2.5-15% (w/v) NaCl (optimum 5-7.5 %). On the basis of 16S rRNA gene sequences, strain X5BTbelonged to the genus Bacillus and showed highest similarity with Bacillus persepolensis HS136T(95.6% 16S rRNA gene sequence similarity) and Bacillus salarius BH169T(95.5 %). The DNA G+C content was 42.4 mol%. The major cellular fatty acids were anteiso-C15: 0and iso-C15: 0and the polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three phospholipids and two glycolipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the isoprenoid quinones were MK-7 (92 %), MK-6 (6 %) and MK-5 (2 %). On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus Bacillus is proposed, with the name Bacillus iranensis sp. nov. The type strain is X5BT(=IBRC 10446T =DSM 23995T). © 2012 IUMS.


Makhdoumi-Kakhki A.,University of Tehran | Makhdoumi-Kakhki A.,Iranian Biological Resource Center | Amoozegar M.A.,University of Tehran | Amoozegar M.A.,Iranian Biological Resource Center | Ventosa A.,University of Seville
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A novel red-pigmented halophilic archaeon, strain EB27T, was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain EB27Twere non-motile and pleomorphic (rods to triangular or disc-shaped). Strain EB27Trequired at least 2.5 M NaCl and 0.1 M MgCl2for growth. Optimal growth was achieved at 4 M NaCl and 0.5 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40°C; it was able to grow at pH 6.0-8.0 and 25- 50°C. 16S rRNA gene sequence analysis showed that strain EB27Tis a member of the family Halobacteriaceae; however, levels of 16S rRNA gene sequence similarity were as low as 90.0, 89.3 and 89.1% to the most closely related haloarchaeal taxa, namely Halalkalicoccus tibetensis DS12T, Halosimplex carlsbadense 2-9-1Tand Halorhabdus utahensis AX-2T, respectively. The DNA G+C content of strain EB27Twas 61 mol%. Strain EB27Tcontained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, together with two minor phospholipids. The only quinone present was MK-8(II-H2). Physiological, biochemical and phylogenetic differences between strain EB27Tand recognized genera of extremely halophilic archaea suggest that this strain represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halovenus aranensis gen. nov., sp. nov. is proposed. The type strain of Halovenus aranensis, the type species of the new genus, is strain EB27T(5IBRC-M 10015T5CGMCC 1.11001T). © 2012 IUMS.


Amoozegar M.A.,University of Tehran | Amoozegar M.A.,Iranian Biological Resource Center | Makhdoumi-Kakhki A.,University of Tehran | Makhdoumi-Kakhki A.,Iranian Biological Resource Center | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

A novel pale pink-pigmented halophilic archaeon, strain DC30T, was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain DC30T were non-motile and pleomorphic, from rods to triangular or disc-shaped. Strain DC30T required at least 1.7 M NaCl and 0.05 M MgCl2 for growth (optimum, 3 M NaCl and 0.1 M MgCl2). The optimum pH and temperature for growth of strain DC30T were pH 7.5 and 40 °C, respectively, although it was capable of growth over pH and temperature ranges of 6.5-8.5 and 25-50 °C, respectively. Analysis of the 16S rRNA gene sequence showed that strain DC30T was a member of the family Halobacteriaceae. However, it had low 16S rRNA gene sequence similarities of 92.4 %, 89.4% and 89.1% to the most closely related haloarchaeal taxa, the type species of the genera Halorubrum, Halogranum and Haloplanus, respectively. The DNA G+C content was 66.0 mol%. Phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, were present. Three minor phospholipids and one unidentified glycolipid were also observed. The only quinone present was MK-8(II-H2). The physiological, biochemical and phylogenetic differences between strain DC30T and other previously described genera of extremely halophilic archaea suggest that strain DC30T represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halopenitus persicus gen. nov., sp. nov. is proposed. The type strain of Halopenitus persicus is DC30T (=IBRC 10041T=KCTC 4046T). © 2012 IUMS.


Makhdoumi-Kakhki A.,University of Tehran | Makhdoumi-Kakhki A.,Iranian Biological Resource Center | Amoozegar M.A.,University of Tehran | Amoozegar M.A.,Iranian Biological Resource Center | Ventosa A.,University of Seville
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

Two Gram-staining-negative, red- and orange-pigmented, non-motile, rod-shaped, extremely halophilic bacteria, designated strains CB7T and DGOT, were isolated from Aran-Bidgol salt lake, Iran. Growth occurred at NaCl concentrations of between 2 and 5 M NaCl and the isolates grew optimally with 3 M NaCl. The optimum pH and temperature for growth of the two strains were pH 7.5 and 37 °C, and they were able to grow over pH and temperature ranges of pH 6-8 and 25-50 °C. The predominant fatty acids of the two isolates were C18: 1ω7c, iso-C15: 0 and summed feature 3 (C16: 1ω7c and/or iso-C15: 0 2-OH). The polar lipid pattern of the two isolates consisted of diphosphatidylglycerol, phosphatidylcholine, three unidentified lipids, one unidentified aminolipid and three unidentified glycolipids. The only quinone present was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains CB7T and DGOT were 64.8 and 65.6 mol%, respectively. 16S rRNA gene sequence analysis indicated that strains CB7T and DGOT were related to Salinibacter ruber in the phylum Bacteroidetes. Levels of 16S rRNA gene sequence similarity between strains CB7T and DGOT and Salinibacter ruber DSM 13855T were 93.2 and 93.6 %, respectively. The two novel strains shared 98.9% 16S rRNA gene sequence similarity. DNA-DNA hybridization experiments between strains CB7T and DGOT and Salinibacter ruber DSM 13855T indicated levels of relatedness of 44 and 52 %, respectively, while the level of relatedness between the two new isolates was 53 %. Chemotaxonomic data supported the placement of strains CB7T and DGOT in the genus Salinibacter. DNA-DNA hybridization studies and biochemical and physiological characterization allowed strains CB7T and DGOT to be differentiated from Salinibacter ruber and from each other. They are therefore considered to represent two novel species of the genus Salinibacter, for which the names Salinibacter iranicus sp. nov. (type strain CB7T=IBRC-M 10036T=CGMCC 1.11003T) and Salinibacter luteus sp. nov. (type strain DGOT=IBRC-M 10423T=CGMCC 1.11002T) are proposed. Emended descriptions of the genus Salinibacter and of Salinibacter ruber are also presented. © 2012 IUMS.


Ghahremaninejad F.,Kharazmi University | Khalili Z.,Kharazmi University | Maassoumi A.A.,Iranian Research Institute of Forests and Rangelands | Mirzaie-Nodoushan H.,Iranian Research Institute of Forests and Rangelands | Riahi M.,Iranian Biological Resource Center
American Journal of Botany | Year: 2012

Premise of the study: The classification of the genus Salix has historically been intrinsically difficult due to its propensity toward plasticity and high variation in diagnostic morphological characters. We investigated leaf epidermal characteristics, focusing on the stomatal apparatus because it may provide critical insights into the evolution and taxonomy of Salix and its closely related genera. • Methods: Light microscopy was used to examine the leaf epidermal features in 32 taxa of Salix. • Key results: Characters such as shape, size, and density of stomatal complexes were very useful in differentiating Salix species. Variation in features of stomatal apparatus in Salix is wider than previously known. Moreover, the type of stomatal complex proved to be very helpful in discriminating Chosenia as members of the genus Salix. • Conclusions: The results of the present study support the placement of Chosenia within Salix and the combining of subgenera Chamaetia and Vetrix because of similarities in their unique stomatal apparatus. © 2012 Botanical Society of America.


Dolatyari A.,Iranian Biological Resource Center | Valles J.,University of Barcelona | Naghavi M.R.,University of Tehran | Shahzadeh Fazeli S.A.,Iranian Biological Resource Center | Shahzadeh Fazeli S.A.,Royan Institute for Reproductive Biomedicine
Plant Systematics and Evolution | Year: 2013

Somatic chromosome numbers of 47 accessions representing 28 Artemisia species are provided from Iran. Two basic chromosome numbers, x = 8, 9, each with diploid, tetraploid and hexaploid levels, were found. Different chromosome numbers, 2n = 16, 16 + 1B, 16 + 5B, 32, 48, and 2n = 18, 18 + 1B, 19, 36, 36 + 1B, 36 + 2B, 37, 49 + 2B, 49 + 3B, 51 + 6B, 54, 54 + 1B, 54 + 3B, 54 + 5B, in studied accessions were identified. Chromosome numbers are reported for the first time in three species, counts in four species are new for Iran, and other counts have been thoroughly compared to previous data. Forty percent of the studied accessions are polyploid and B-chromosome(s) are reported in 17 % of accessions. Aneuploidy and aneusomy are other relevant cytological phenomena. Some karyological parameters, total karyotype length, karyotype formula, mean value of centromeric indices, mean arm ratio, A 1 and A 2 indices, were estimated to characterize the karyotypes numerically. A 1 and A 2 indices showed that karyotypes tend to be asymmetric in polyploid and dysploid taxa. PCA analysis of all karyological parameters has shown some systematic and evolutionary implications. The consideration of all these chromosome numbers and cytogenetic mechanisms has led us to infer the different patterns of chromosomal evolution in the genus. © 2013 Springer-Verlag Wien.


Nikfarjam L.,Iranian Biological Resource Center | Farzaneh P.,Iranian Biological Resource Center
Cell Journal | Year: 2012

One of the main problems in cell culture is mycoplasma infection. It can extensively affect cell physiology and metabolism. As the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. This review will provide valuable information about: 1. the ways in which cells are contaminated and the frequency and source of mycoplasma species in cell culture; 2. the ways to prevent mycoplasma contamination in cell culture; 3. the importance of mycoplasma tests in cell culture; 4. different methods to identify mycoplasma contamination; 5. the consequences of mycoplasma contamination in cell culture and 6. available methods to eliminate mycoplasma contamination. Awareness about the sources of mycoplasma and pursuing aseptic techniques in cell culture along with reliable detection methods of mycoplasma contamination can provide an appropriate situation to prevent mycoplasma contamination in cell culture.


Ghafoori H.,Guilan University | Askari M.,Guilan University | Sarikhan S.,Iranian Biological Resource Center
Extremophiles | Year: 2016

This study reports the purification and characterization of an extracellular haloalkaline serine protease from the moderately halophilic bacterium, Bacillus iranensis, strain X5B. The enzyme was purified to homogeneity by acetone precipitation, ultrafiltration and carboxymethyl (CM) cation exchange chromatography, respectively. The purified protease was a monomeric enzyme with a relative molecular mass of 48–50 kDa and it was inhibited by PMSF indicating that it is a serine-protease. The optimum pH, temperature and NaCl concentration were 9.5, 35 °C and 0.98 M, respectively. The enzyme showed a significant tolerance to salt and alkaline pH. It retained approximately 50 % of activity at 2.5 M NaCl and about 70 % of activity at highly alkaline pH of 11.0; therefore, it was a moderately halophilic and also can be activated by metals, especially by Ca2+. The specific activity of the purified protease was measured to be 425.23 μmol of tyrosine/min per mg of protein using casein as a substrate. The apparent Km and Vmax values were 0.126 mM and 0.523 mM/min, respectively and the accurate value of kcat was obtained as 3.284 × 10−2 s−1. These special and important characteristics make this serine protease as valuable tool for industrial applications. © 2015, Springer Japan.

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