Nikkhoi S.K.,Tarbiat Modares University |
Rahbarizadeh F.,Tarbiat Modares University |
Ahmadvand D.,Iran University of Medical SciencesTehran
Protein Expression and Purification | Year: 2017
Variable heavy chain of HcAb (VHH), the smallest intact antibody fragment, possesses sub-nanomolar affinity to antigens. In spite of conventional antibodies, these fragments recognize concave and linear epitopes. VHHs are one the best weapon for targeted drug delivery in nanomedicine and biopharmaceutics. HER2 is overexpressed in 20–25% of breast and ovarian cancers. For many reasons, HER2 is a prominent target for drug delivery to breast tumor. In this study, we designed a robust prokaryotic expression system to express functional VHHs against HER2 receptor. This system showed high recombinant yields besides purified VHHs flow cytometry verified great capabilities of these molecules to pinpoint ecto-domain of HER2 receptor in MC4L2 HER2+ while insignificant non-specific binding to MC4L2 HER2-confirm nanobodies trivial cross-reaction. In the next step, we evaluated cooperative effect of four distinctive VHHs (oligoclonal VHHs) targeting different epitopes on HER2. As our result proved, using oligoclonal nanobodies as targeting moiety enhance targeting efficacy in comparison with monoclonal VHH. © 2016 Elsevier Inc.
Sharifi G.,Shahid Beheshti University |
Bakhtevari M.H.,Shahid Beheshti University |
Alghasi M.,Iran University of Medical SciencesTehran |
Saberi M.,Erfan General HospitalTehran |
And 3 more authors.
Clinical Neurology and Neurosurgery | Year: 2015
Abstract Background Intrasellar location of schwannoma is extremely rare, although intracranial schwannomas are common in the central nervous system. The aim of the present study is to describe a calcified intrasellar schwannoma case. Materials and methods We represent a 45-year-old woman who had suffered from headaches; right side facial pain and visual disturbance which had worsen during the last week prior to admission. Physical examinations were normal except for the bitemporal visual field hemianopia which match with perimetry examination. MRI demonstrated an unusual seemingly calcified mass lesion in the sellar region which was mimicking pituitary macro adenoma. Result Total resection of the tumor achieved through endoscopic transnasal transsphenoidal approach by extracapsular dissection and pathologic examination of the tumor revealed calcified schwannoma. Conclusion The differential diagnoses of sellar and suprasellar lesions include pituitary adenomas, craniopharyngiomas, meningiomas, and many others. However, schwannoma is not usually included, because the occurrence of schwannoma in the sellar or suprasellar region is extremely rare. Only few cases of intrasellar schwannomas have been reported in the literature, all of which presented a suprasellar extension similar to that of our case. Fascinating surgical point is managing very firm tumor through transsphenoidal corridor which we handle it by very sharp, debulking and extracapsular removal. © 2015 Elsevier B.V.
Sabour H.,University of Tehran |
Javidan A.N.,University of Tehran |
Latifi S.,University of Tehran |
Shidfar F.,Iran University of Medical SciencesTehran |
And 4 more authors.
Journal of Spinal Cord Medicine | Year: 2015
Context: Omega-3 fatty acids have been recently proposed to induce neural improvement in patients with spinal cord injury (SCI) while affecting some hormones including leptin and adiponectin. Objectives: We tried to evaluate the effect of omega-3 fatty acids on circulatory concentrations of leptin and adiponectin among these patients. Design: This study is a double-blinded randomized clinical trial with intervention duration of 14 months. Setting: A tertiary rehabilitation center. Participants: Total of 104 patients with SCI who did not meet our exclusion criteria entered the study. Those with history of diabetes, cancer, endocrinology disease, acute infection, and use of special medications were excluded. Patients were divided randomly into the treatment and control group by using permuted balanced block randomization. Intervention: The treatment group received two MorDHA® capsules per day (each capsule contain 465 mg of docosahexaenoic acid (DHA) and 63 mg of eicosapentaenoic acid (EPA)) for 14 months while the control group received placebo capsules with similar color, shape, and taste. Main outcomes measures: Leptin and adiponectin concentrations in plasma were measured at the beginning of trial and then after 6 and 14 months. Results: Fourteen months of treatment with DHA and EPA did not influence concentrations of leptin but adiponectin level was significantly decreased (P: 0.03). Weight was positively correlated with leptin level at stage 0 of trial (P: 0.008, r = 0.41) while this association was attenuated through stages of trial after intervention. Conclusion: Our data show that omega-3 fatty acids may not affect plasma concentrations of leptin but adiponectin level is decreased in patients with SCI. Moreover, this intervention influences the linear relationship between weight and leptin after 14 months administration of DHA and EPA. © The Academy of Spinal Cord Injury Professionals, Inc. 2015.
Teimourian S.,Iran University of Medical SciencesTehran |
Teimourian S.,University of Tehran |
Teimourian S.,Islamic Azad UniversityTehran |
Masoudzadeh N.,University of Tehran |
Masoudzadeh N.,Islamic Azad UniversityTehran
European Journal of Pharmacology | Year: 2015
Abstract Crohn's disease (CD), a subcategory of inflammatory bowel disease, is an immune-related disorder characterized by inflammation of the gastrointestinal mucosa, which can take place in any region along the alimentary tract. The most important gene involved in the etiology of CD is NOD2/CARD15 located on chromosome 16. It has been shown that CARD15 is overexpressed in monocytes of CD patients. The common treatment for the disease is anti-TNF-alpha drugs, the most hopeful of which are probably infliximab and etanercept. Infliximab rapidly reduces signs and symptoms of active Crohn's disease. In contrast, etanercept shows no such effect. In the present study, we evaluated the effects of the CARD15 gene overexpression in monocytic cell line U937 in the production of anti-inflammatory cytokine, IL-10, and proinflammatory cytokine, Il-1 beta, produced after incubation with infliximab, adalimumab, and etanercept separately. Our results show that infliximab and adalimumab significantly decreased IL-10 and IL-1beta secretion levels. However, etanercept inhibition of secretion was less compared with infliximab or adalimumab. In all three cases, suppression of cytokine production is reduced by CARD15 overexpression. © 2015 Elsevier B.V.
Tajmanesh M.,Iran University of Medical SciencesTehran |
Aryaeian N.,Iran University of Medical SciencesTehran |
Hosseini M.,University of Tehran |
Mazaheri R.,University of Tehran |
Kordi R.,University of Tehran
Lipids | Year: 2015
Abstract This study investigated the effect of conjugated linoleic acid (CLA) on the aerobic capacity and anthropometric measurements of humans. Although this effect has been shown in animal studies, human studies have reported controversial results. In this double-blind, placebo-controlled, randomized clinical trial, 80 non-trained healthy young men received a 50:50 mixture of cis-9,trans-11 and trans-10 cis-12 CLA (CLA 4 × 0.8 g day-1) ora placebo (PLA; soybean oil) in an 8-week intervention. Maximal oxygen consumption (VO2 max), time to exhaustion, weight, body mass index (BMI) and waist circumference (WC) were measured. CLA had no effect on VO2 max (p = 0.5) also no change was seen in time to exhaustion (p = 0.51), weight (p = 0.7), BMI (p = 0.7) and WC (p = 0.8) vs PLA. Our results suggest that CLA has no significant effect on VO2 max, time to exhaustion and anthropometric measurements in untrained healthy young male students. © 2015 AOCS.
Mohseni-Bandpi A.,Shahid Beheshti University |
Kakavandi B.,Ahvaz Jundishapur University of Medical Sciences |
Kalantary R.R.,Iran University of Medical SciencesTehran |
Azari A.,University of Tehran |
Keramati A.,University of Tehran
RSC Advances | Year: 2015
Magnetic nanoparticles (MNPs) have recently been adopted by researchers in the field of adsorption/biosorption for separation of pollutants from aqueous solutions. In this paper, chitosan was impregnated with magnetite nanoparticles through a chemical co-precipitation method to fabricate hybrid adsorbents of Fe3O4-chitosan. The physicochemical and structural properties of the adsorbent were characterized, and then the performance of the adsorbent was evaluated for fluoride removal from water. The operational factors affecting the adsorption process, including pH, contact time, adsorbent dosage, initial fluoride concentration, and temperature, were studied. Various isotherm and kinetic models were also used to evaluate the fit of the experimental data with the modeled results. The equilibrium data were well described by the Freundlich model. The kinetics of the adsorption process followed the pseudo-second-order model. Recycling results suggested that the Fe3O4-chitosan particles maintain a great reusability potential for five consecutive cycles. Findings also showed that the Fe3O4-chitosan can be easily regenerated via acid treatment. The results of the present work highlighted the potential of using the Fe3O4-chitosan magnetic composite for the removal of fluoride from water. In conclusion, Fe3O4-chitosan can be considered as an appropriate adsorbent for fluoride removal from water, because it can be separated both quickly and easily, it has high efficiency, and it does not lead to secondary pollution. This journal is © The Royal Society of Chemistry.
Arami M.K.,Tarbiat Modares University |
Arami M.K.,Iran University of Medical SciencesTehran |
Hajizadeh S.,Tarbiat Modares University |
Semnanian S.,Tarbiat Modares University
Brain Research | Year: 2016
Glutamatergic synapses are shown to mature during activity and development. In order to further explore how glutamate can change the excitability of noradrenergic neurons of locus coeruleus (LC) and to better understand the involvement of Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors complements across the LC, we investigated developmental changes in their activity during first postnatal weeks. Spontaneous and evoked excitatory postsynaptic currents (sEPSC and eEPSCs) were recorded in neurons of LC slices from 7, 14 and 21 days old rats using the whole cell patch clamp method. Also, the AMPA/NMDA current ratio (A/N) was measured. A pronounced AMPAR and NMDAR components mediated involvement in synaptic transmission were seen from the first postnatal week. Over this period of development, we have demonstrated that AMPA sEPSCs show an increase in frequency without major changes in their amplitude, while NMDA sEPSCs show an increase in frequency with a major change in amplitude. Neither the probability of release nor the AMPA/NMDA ratio was found to change significantly with age. It is concluded that NMDAR activity as well as AMPAR activity may be involved in coerulear excitability and modulatory effect during postnatal development. © 2016
Farzadkia M.,Iran University of Medical SciencesTehran |
Bazrafshan E.,Zahedan University of Medical Sciences |
Esrafili A.,Iran University of Medical SciencesTehran |
Yang J.-K.,Kwangwoon University |
Shirzad-Siboni M.,Iran University of Medical SciencesTehran
Journal of Environmental Health Science and Engineering | Year: 2015
Abstract Metronidazole (MNZ) is a brand of nitroimidazole antibiotic, which is generally used in clinical applications and extensively used for the treatment of infectious diseases caused by anaerobic bacteria and protozoans. The aim of this investigation was to degrade MNZ with illuminated TiO2 nanoparticles at different catalyst dosage, contact time, pH, initial MNZ concentration and lamp intensity. Maximum removal of MNZ was observed at near neutral pH. Removal efficiency was decreased by increasing dosage and initial MNZ concentration. The reaction rate constant (k obs ) was decreased from 0.0513 to 0.0072 min-1 and the value of electrical energy per order (EEo) was increased from 93.57 to 666.67 (kWh/m3) with increasing initial MNZ concentration from 40 to 120 mg/L, respectively. The biodegradability estimated from the BOD5/COD ratio was increased from 0 to 0.098. The photocatalyst demonstrated proper photocatalytic activity even after five successive cycles. Finally, UV/TiO2 is identified as a promising technique for the removal of antibiotic with high efficiency in a relatively short reaction time. © 2015 Farzadkia et al.; licensee BioMed Central.
Haghighatpanah M.,Islamic Azad University at Lahijan |
Mozaffari Nejad A.S.,Hamadan University of Medical Sciences |
Mojtahedi A.,Guilan University |
Amirmozafari N.,Iran University of Medical SciencesTehran |
Zeighami H.,University of Zanjan
Journal of Global Antimicrobial Resistance | Year: 2016
Escherichia coli is an important cause of hospital-acquired infections worldwide. Antimicrobial resistance leads to treatment failure of hospital infections caused by E. coli. Production of extended-spectrum β-lactamases (ESBLs) is one of the major causes of antibiotic resistance in these bacteria. This study aimed to investigate the frequency of blaTEM and blaCTX-M genes in ESBL-producing E. coli strains isolated from clinical specimens of patients admitted to six hospitals in the north of Iran. A total of 160 E. coli strains were isolated from various clinical samples of hospitalised patients. Antibiotic resistance patterns were determined by the Kirby–Bauer disk diffusion method. The double-disk phenotypic confirmatory test was carried out amongst β-lactam-resistant isolates to detect ESBL-producing strains. Plasmid DNA of ESBL-producing strains was extracted and subjected to PCR for detection of the blaTEM and blaCTX-M genes, and isolates were extensively verified by sequencing. The highest resistance rate was to amoxicillin; all E. coli isolates (100%) were susceptible to imipenem. Amongst the 160 clinical E. coli isolates, 83 (51.9%) were ESBL-positive, of which 27 (32.5%) and 72 (86.7%) were positive for blaTEM and blaCTX-M, respectively. This study is the first report of an ESBL phenotype disseminated in hospitals in the north of Iran. These findings showed that there was a direct relationship between the development of resistance to β-lactam antibiotics and production of TEM and CTX-M enzymes. © 2016 International Society for Chemotherapy of Infection and Cancer
Abdi A.,University of Tabriz |
Karimi A.,University of Tabriz |
Karimi A.,Iran University of Medical SciencesTehran |
Razzaghi M.,University of Tabriz
Journal of Environmental Chemical Engineering | Year: 2016
In this research, removing dissolved oxygen (DO) from water by means of granular biocatalyst (GB) was investigated. GB was prepared by granulation of glucose oxidase (GOx) adsorbed on MnO2 micro-sized particles by calcium alginate. Prepared biocatalyst was able to remove DO and decompose produced hydrogen peroxide simultaneously. Factors affecting DO removal including temperature, pH and glucose concentration were studied; at the optimum condition which was achieved to be 30 °C, 6.85 and 80 mM respectively, DO was removed completely. Stability of enzymatic activity of GB was investigated. Besides, Water deoxygenation was studied in a packed bed reactor by GB utilization at optimum operating condition. The highest deoxygenation rate was observed at feed flow rate of 375 mL/min. Residence time distribution of packed bed reactor was calculated at two flow rates with pulse injection of tracer into reactor inlet. © 2016 Published by Elsevier Ltd.