Iran Silkworm Research Center

Rasht, Iran

Iran Silkworm Research Center

Rasht, Iran
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Salehi Nezhad M.,Islamic Azad University | Mirhosseini S.Z.,Guilan University | Gharahveysi S.,Islamic Azad University | Mawajpour M.,Iran Silkworm Research Center | Seidavi A.R.,Islamic Azad University at Rasht
Journal of Food, Agriculture and Environment | Year: 2010

This experiment was conducted in order to comparison of all 51 peanut cocoon silkworm strains of Islamic Republic of Iran in gene bank and selection and introduction of the best lines available for next breeding programs. Fifty-one silkworm strains were used in the present study. The batches of 250 silkworm larvae were reared. Based on reproductive characters strains were assessed on different parameters including hatchability percentage, number of laid eggs, number of fertilized eggs, number of unfertilized eggs, and number of unhatched eggs using the evaluation index and sub-ordinate function methods. The hatchability percentage of the 105 (98.400%), M2-6-22-2 (98.400%), B2-09 (97.733%), 101433-1-4 (97.500%) and T5-M (97.400%) strains remained significantly at upper level than that of other strains, respectively. Totally, M2-6-22-2 (330.591), T5-M (329.463), 105 (316.794), 101433-1-4 (299.172) and 1126[111] (297.447) showed higher evaluation index values. Also, 1524(3.375), M-1-2[5] (3.112), 151 [103×M1-1] (3.083), M2-6-22[ 107] (2.919) and N19 (2.763) showed higher sub-ordinate function values.


Salehi Nezhad M.,Islamic Azad University | Mirhosseini S.Z.,Guilan University | Gharahveysi S.,Islamic Azad University | Mavvajpour M.,Iran Silkworm Research Center | Seidavi A.R.,Islamic Azad University at Rasht
Journal of Food, Agriculture and Environment | Year: 2010

The present study was undertaken to ascertain the magnitude of genetic diversity among all 51 silkworm genotypes and estimate the genetic variation for economical traits to augment the utilization of silkworm genetic resources for silkworm breeding programs. Various methods generated similar dendrograms. The dendrograms obtained from hierarchical cluster analysis of Iranian silkworm strains based on studied traits were presented. However, the strains of the same origin did not group together, demonstrating they can have different biological and development performance. The first and second groups divided into three sub-groups separately. Three sub-groups of the first group included 8, 15 and 1 strains, respectively. Other strains were grouped together and far from other silkworm strains, indicating they might be suitable for future crossings, maintenance of parental strains, and hybridizations with oval cocoon strains so as to maximize heterosis and to avoid depression inbreeding.


Mirhosseini S.Z.,Guilan University | Nematollahian S.,Iran Silkworm Research Center | Ghanipoor M.,Iran Silkworm Research Center | Seidavi A.,Islamic Azad University at Rasht
Biological Research | Year: 2010

Five Iranian native silkworm groups: Baghdad, Khorasan Orange, Guilan Orange, Khorasan Pink, Khorasan Lemon, and 107 and 110 commercial lines (12 families from each breed) were randomly selected and reared during 2003-2005 (five generations in spring and autumn). In each family, 30 male and 30 female cocoons were individually recorded for weight, shell weight and shell ratio. From among the native groups, the highest average in all three traits belonged to Baghdad and Khorasan Pink, and the lowest to Khorasan Orange and Khorasan Lemon. From among the commercial lines, the highest average in all three traits belonged to 107. In comparing heritability for cocoon weight in native groups, the highest heritability belonged to Guilan Orange (0.5147) and Khorasan Orange (0.5036) and the lowest heritability belonged to Khorasan Pink (0.0967). In the two other traits, the highest heritability belonged to Khorasan Orange and Baghdad and the lowest to Khorasan Pink. In the commercial lines, line110 had higher heritability than line107 for cocoon weight and cocoon shell weight. In all the groups, genetic correlations between cocoon weight and cocoon shell weight were high, expect for the Baghdad group. There was medium or low genetic correlation among cocoon weight, cocoon shell weight and cocoon shell ratio.


Salehi Nezhad M.,Islamic Azad University | Mirhosseini S.Z.,Guilan University | Gharahveysi S.,Islamic Azad University | Mavvajpour M.,Iran Silkworm Research Center | And 2 more authors.
African Journal of Biotechnology | Year: 2010

The aim of this experiment was to study and classify all 51 pure lines of Iran silkworm germplasm based on larval traits and identification of pure lines relationships. The average linkage between two groups is considered as the average of distance between all pairs of cases with one number from each group. Hierarchical clustering analysis was carried out by considering all studied parameters together. The grouping methods allowed the study to subdivide the observations into several subgroups in such a way that homogeneity was obtained inside the subgroups and heterogeneity among the subgroups. Various methods generated similar dendograms. On the basis of these dendrograms, analyzed strains were divided into 2 distinct groups. Frequent divisions were also observed in major groups. The first group was divided into two sub groups including three and eight strains, respectively. The second major group was divided into two sub groups which include 4 and 36 strains, respectively. © 2010 Academic Journals.


Nezhad M.S.,Islamic Azad University | Mirhosseini S.Z.,Guilan University | Gharahveysi S.,Islamic Azad University | Mavvajpour M.,Iran Silkworm Research Center | Seidavi A.R.,Islamic Azad University at Rasht
Asian Journal of Animal and Veterinary Advances | Year: 2010

The present study aims at shedding more light to larval duration and development of silkworm lines from Iranian silkworm gene bank and comparison of the results using statistical models for selection of the superior strains. Feeding and other conditions of larval rearing were conducted following the standard procedure and all germplasm strains were reared under standards protocols in all rearing steps. From obtained results, it is showed that the larval duration of the 101 (608.000 h), 5118x10133-3-3 (588.670 h), 307-300-2 (584.000 h), 105 (584.000 h) and 31 (584.000 h) strains remained significantly at upper level than other strains, respectively. The feeding larval duration in B2-09 (574.000 h), N19 (533.000 h), 1433-9 (525.000 h), BH-2 (517.330 h) and 1433-15 (511.330 h) strains increased significantly in comparison with other strains. Molting larval duration remained significantly at upper level in I 20 (197.670 h), 107-K (113.000 h), Black Larvae-White Cocoon (104.000 h), 101(104.000 h) and Shaki (103.000 h) increased significantly in comparison with other strains. From obtained results, it is showed the 1-3 instars larval duration of the Black-White (292.670 h), 101 (290.000 h), 1003-5 (288.670 h), 101xF6 (286.000 h) and 31 (286.000 h) strains remained significantly at upper level than other strains, respectively. Totally, 7409 (577.881), Black Larvae-White Cocoon (577.508), 236 (570.769), M-1-2(5) (568.583) and T5-M (566.602) showed higher evaluation index values. Also, 7409 (5.374), 236 (5.267), T5-M (5.183), 113-K (5.163) and White Larvae-Yellow Cocoon (5.027) showed higher sub-ordinate function values. © 2010 Academic Journals Inc.


Nezhad M.S.,Islamic Azad University at Rasht | Seidavi A.,Islamic Azad University at Rasht | Mirhosseini S.Z.,Guilan University | Mavvajpour M.,Iran Silkworm Research Center
Life Science Journal | Year: 2011

In different breeding programs, knowledge of inbreeding and genotype similarities and differences of various varieties is necessary. In many breeding projects, hybridization planning and in different crosses, there is a need to exist varieties with far and close genetic characteristics to each other. Based on this, it is necessary to identify the similarities between various breed. This experiment aiming to sort Japanese varieties group of Iran silkworm gene bank and to investigate genetic relationships between them based on individual economic characteristics was designed and planned. Data of the mentioned traits had been recorded based on performance tests. In order to group lines based on several important economic traits, Cluster analysis with UPGMA method performed on the studied genotypes using Ntsys-pc software. For each of concerned economic traits, the matrix analogous indicates Japanese varieties distance to each other had been determined and according this phylogenic diagram of this varieties that expressing near or far different varieties of Iran Japanese silkworm based on individual economic traits was drawn. According to the results from this research, the studied varieties can locate in different groups, accurately, and express their distance to each other. © 2011. Zhengzhou University, Marsland Press.


Mohammadi Torkashvand A.,Islamic Azad University at Rasht | Bizhannia A.,Iran Silkworm Research Center | Mavajpour M.,Iran Silkworm Research Center | Haghighat N.,Islamic Azad University at Rasht
Life Science Journal | Year: 2011

Using conventional lime in amending acidic soils reform is expensive. Therefore, the importance of using cheaper resources is seems more necessary. A variety of materials including industries by-products are used in reclamation of acidic soil. By-products with the character of lime not only increase the pH and improvement of soils have special priority, but also increase the fertility with create consistency with the aggregate building sustainable soil, air conditioning, increases porosity and cation exchange capacity and soil reactivity. In this study, the possibility of using paper sludge lime had been investigated in berry orchard improvement under cultivation of sericulture in a field experiment. The results indicating the potential of paper lime sludge as acidic soil modifiers in the replacement of conventional calcite sources such as calcite which can reduce the disposal costs and possible environmental contamination of this by-product.


Molaei M.,Islamic Azad University | Mavvajpour M.,Iran Silkworm Research Center | Merat A.,Iran Silkworm Research Center | Seidavi A.,Islamic Azad University at Rasht
Life Science Journal | Year: 2011

The purpose of this experiment was to investigation on different delay times in starting of feeding in different instars, and their effects on the performance and uniformity of silkworm larvae. Silkworm egg production stages, egg washing, disinfecting, maintenance of silkworm eggs, microscopic tests in order to removing of contaminated samples against pebrin pathogen, first to fifth larval instars rearing, cocoon production framework and cocoon recording was conducted based on standard guidelines. Ten dietary management programs were used as ten treatments. Performance records analyzed using generalized linear models procedure. All the measured indices was compared between different treatments based completely randomized design model. From obtained results, it has showed that among studied methods, the highest level of best cocoon number belonged to 5th treatment (80.75), and 3rd treatment (44.70) remained at lower level than other methods (P>0.05). The highest level of best cocoon weight belonged to 5th treatment (80.75 gr), and 3rd treatment (44.70 gr) remained at lower level than other methods (P>0.05). The highest level of larva weight (5th day of 5th instar) belonged to 3rd treatment (3.48 days), and 9th treatment (3.24 days) remained at lower level than other methods (P<0.05). Among studied methods, the highest level of female cocoon weight belonged to 1st treatment (2.13 gr), and 2nd treatment (1.90 gr) remained at lower level than other methods (P<0.05). The highest female cocoon shell weight belonged to 1st treatment (0.42 gr), and 2nd treatment (0.36 gr) remained at lower level than other methods (P<0.05). Among studied methods, the highest level of female cocoon shell percentage belonged to 9th treatment (24.18%), and 2nd treatment (18.94%) remained at lower level than other methods (P>0.05). The highest level of male cocoon weight belonged to 6th treatment (1.69 gr), and 2nd treatment (1.56 gr) remained at lower level than other methods (P<0.05).


Azizian S.,Guilan University | Mirhoseini S.Z.,Guilan University | Shadparvar A.A.,Guilan University | Ghanipoor M.,Iran Silkworm Research Center | Seidavi A.R.,Islamic Azad University at Rasht
Journal of Food, Agriculture and Environment | Year: 2011

Increase of inbreeding could cause reduction in performance of some economic traits of animal populations. Thereby in line breeding there is an attempt for prevention of more inbreeding in populations. The level of inbreeding and its relationship to the economic traits (cocoon weight, cocoon shell weight and cocoon shell percentage) of 4 lines of Iranian silkworm populations (107 and 153 of Japanese origin and 110 and 154 of Chinese origin) using 3 regression models were estimated. In the first model linear effect of inbreeding, in the second model linear and quadratic effect of inbreeding and in the third model linear, quadratic and cubic effect of inbreeding on the traits were investigated with presence of line, family and gender effects. Pedigree information of 40,717 silkworms collected during 9 generations during 2001-2006 in Iran Silkworm Research Center were studied. The average of inbreeding coefficient of total population was 9.626%. Except for line 153 in the second model and line 107 in the third model, inbreeding had positive effect on cocoon shell percentage. Model 1 with linear inbreeding effect had more significancy (p<0.0001) and less complexity for study of inbreeding effect on cocoon shell percentage. On the basis of Model 1, in all studied lines, except for line 154, inbreeding effect on cocoon weight and cocoon shell weight was not significant. On the basis of Model 3, inbreeding had negative effect on cocoon weight and cocoon shell weight in line 110. The estimates of inbreeding effect on studied traits was intensively affected by the type of statistical model.


Salehi Nezhad M.,Islamic Azad University | Mirhosseini S.Z.,Guilan University | Gharahveysi S.,Islamic Azad University | Mavvajpour M.,Iran Silkworm Research Center | Seidavi A.R.,Islamic Azad University at Rasht
Biotechnology | Year: 2010

This study was conducted in ordered to analyze of phylogenetic relationships and determination of relationship amounts in all peanut cocoon strains of Islamic Republic of Iran germplasm based on reproductive characteristics. Hierarchical agglomerative clustering was done by using NTSYS-pc, version 2.02e based on complete, single, UPGMA, UPGMC, FLEXI approaches and SAS-pc based on WARD and average approaches. However, method of average linkage between groups under UPGMA (Unweighted Pair-Group Method using Arithmetic average) was considered as major and final protocol for data conclusion and the resulting clusters were expressed as dendrograms. Various methods generated similar dendograms. This study reveals the phylogenetic relationship of peanut cocoon strains of Iran germplasm. Based on data from studied characters, we constructed dendrograms that resolved the 51 silkworm strains into 2 major clusters. However, the strains of the same origin did not grouped together, demonstrating they can have different biological and development performance. First cluster divided into one sub-group included five strains. However, second cluster divided into two sub-groups. Other strains were grouped together and far from other silkworm strains, indicating they might be suitable for future crossings, maintenance of parental strains and hybridizations with oval cocoon strains so as to maximize heterosis and to avoid depression inbreeding. © 2010 Asian Network for Scientific Information.

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