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Rubio J.,Jaume I University | Alfonso I.,IQAC | Burguete M.I.,Jaume I University | Luis S.V.,Jaume I University
Chemical Communications | Year: 2012

The formation of soluble nano-spheres or stable hydrogels through the self-assembling of a simple gemini amphiphilic pseudopeptide can be controlled by the tuning of the hydrophilic/hydrophobic interactions in aqueous medium. © 2012 The Royal Society of Chemistry. Source


Nogueira D.R.,University of Barcelona | Mitjans M.,University of Barcelona | Busquets M.A.,University of Barcelona | Perez L.,IQAC | Vinardell M.P.,University of Barcelona
Langmuir | Year: 2012

Amino acid-based surfactants constitute an important class of natural surface-active biomolecules with an unpredictable number of industrial applications. To gain a better mechanistic understanding of surfactant-induced membrane destabilization, we assessed the phospholipid bilayer-perturbing properties of new cationic lysine-based surfactants. We used erythrocytes as biomembrane models to study the hemolytic activity of surfactants and their effects on cells' osmotic resistance and morphology, as well as on membrane fluidity and membrane protein profile with varying pH. The antihemolytic capacity of amphiphiles correlated negatively with the length of the alkyl chain. Anisotropy measurements showed that the pH-sensitive surfactants, with the positive charge on the α-amino group of lysine, significantly increased membrane fluidity at acidic conditions. SDS-PAGE analysis revealed that surfactants induced significant degradation of membrane proteins in hypo-osmotic medium and at pH 5.4. By scanning electron microscopy examinations, we corroborated the interaction of surfactants with lipid bilayer. We found that varying the surfactant chemical structure is a way to modulate the positioning of the molecule inside bilayer and, thus, the overall effect on the membrane. Our work showed that pH-sensitive lysine-based surfactants significantly disturb the lipid bilayer of biomembranes especially at acidic conditions, which suggests that these compounds are promising as a new class of multifunctional bioactive excipients for active intracellular drug delivery. © 2012 American Chemical Society. Source


Nogueira D.R.,University of Barcelona | Mitjans M.,University of Barcelona | Infante M.R.,IQAC | Vinardell M.P.,University of Barcelona
Acta Biomaterialia | Year: 2011

Surfactants are among the most versatile and widely used excipients in pharmaceuticals. This versatility, together with their pH-responsive membrane-disruptive activity and low toxicity, could also enable their potential application in drug delivery systems. Five anionic lysine-based surfactants which differ in the nature of their counterion were studied. Their capacity to disrupt the cell membrane was examined under a range of pH values, concentrations and incubation times, using a standard hemolysis assay as a model for endosomal membranes. The surfactants showed pH-sensitive hemolytic activity and improved kinetics at the endosomal pH range. Low concentrations resulted in negligible hemolysis at physiological pH and high membrane lytic activity at pH 5.4, which is in the range characteristic of late endosomes. With increasing concentration, the surfactants showed an enhanced capacity to lyse cell membranes, and also caused significant membrane disruption at physiological pH. This observation indicates that, at high concentrations, surfactant behavior is independent of pH. The mechanism of surfactant-mediated membrane destabilization was addressed, and scanning electron microscopy studies were also performed to evaluate the effects of the compounds on erythrocyte morphology as a function of pH. The in vitro cytotoxicity of the surfactants was assessed by MTT and NRU assays with the 3T3 cell line. The influence of different types of counterion on hemolytic activity and the potential applications of these surfactants in drug delivery are discussed. The possibility of using pH-sensitive surfactants for endosome disruption could hold great promise for intracellular drug delivery systems in future therapeutic applications. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. Source


Nogueira D.R.,University of Barcelona | Mitjans M.,University of Barcelona | Moran M.C.,University of Barcelona | Perez L.,IQAC | Vinardell M.P.,University of Barcelona
Amino Acids | Year: 2012

Many strategies for treating diseases require the delivery of drugs into the cell cytoplasm following internalization within endosomal vesicles. Thus, compounds triggered by low pH to disrupt membranes and release endosomal contents into the cytosol are of particular interest. Here, we report novel cationic lysine-based surfactants (hydrochloride salts of Nε and Nα-acyl lysine methyl ester) that differ in the position of the positive charge and the length of the alkyl chain. Amino acid-based surfactants could be promising novel biomaterials in drug delivery systems, given their biocompatible properties and low cytotoxic potential. We examined their ability to disrupt the cell membrane in a range of pH values, concentrations and incubation times, using a standard hemolysis assay as a model of endosomal membranes. Furthermore, we addressed the mechanism of surfactant-mediated membrane destabilization, including the effects of each surfactant on erythrocyte morphology as a function of pH. We found that only surfactants with the positive charge on the a-amino group of lysine showed pH-sensitive hemolytic activity and improved kinetics within the endosomal pH range, indicating that the positive charge position is critical for pH-responsive behavior. Moreover, our results showed that an increase in the alkyl chain length from 14 to 16 carbon atoms was associated with a lower ability to disrupt cell membranes. Knowledge on modulating surfactant-lipid bilayer interactions may help us to develop more efficient biocompatible amino acid-based drug delivery devices. © 2011 Springer-Verlag. Source


News Article | April 28, 2016
Site: http://phys.org/biology-news/

A new study led by ICTA-UAB (Universitat Autònoma de Barcelona) researcher Víctor Sarto and colleagues from the Institute of Advanced Chemistry of Catalonia (CSIC-IQAC) has described for the first time in two centuries of knowledge a case of evolutionary convergence in the order of butterflies (Lepidoptera), certainly representing an evolutionary breakthrough to what has been known about their sexual communication. The research has discovered important behavior and physiological changes in the mating process of the moth Paysandisia archon (Castniidae). This neotropical moth that reached Europe in 2001 from Argentina (also inhabiting Uruguay and Brasil) breaks the known sexual rules by behaving like a diurnal butterfly.

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