Ip Laboratorio Nacional Of Investigacao Veterinaria

Lisbon, Portugal

Ip Laboratorio Nacional Of Investigacao Veterinaria

Lisbon, Portugal
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Martins H.M.,Ip Laboratorio Nacional Of Investigacao Veterinaria | Almeida I.,Direccao Geral de Veterinaria | Camacho C.,Ip Laboratorio Nacional Of Investigacao Veterinaria | Costa J.M.,Direccao Geral de Veterinaria | Bernardo F.,Polo Universitario da Ajuda
Mycotoxin Research | Year: 2012

Results of a 2-year (2009-2010) survey on the occurrence of ochratoxin A (OTA) in swine feed and in feed for laying hens in Portugal are reported. A total of 664 samples (478 swine feed, 186 feed for laying hens) were analyzed by a HPLC method using fluorescence detection with 2 μg kg-1 as detection limit. In swine feed, 31 samples (6.49%) were positive for OTA. In feed for laying hens, 12 samples (6.45%) were OTA-positive. The average levels of contamination were low, with median values of positive samples at 3-4 μg kg-1 in both years and both commodities, although a few samples contained exceptionally high levels (maximum 130 μg kg-1). Only the maximum level sample (swine feed) contained OTA at a concentration exceeding the European Commission guidance value. The remaining OTA concentrations found in feed samples were much lower than the guidance values. © 2012 Society for Mycotoxin Research and Springer.


Martins H.M.L.,Ip Laboratorio Nacional Of Investigacao Veterinaria | Almeida I.F.M.,Direccao Geral de Veterinaria | Camacho C.R.L.,Ip Laboratorio Nacional Of Investigacao Veterinaria | Santos S.M.O.,Ip Laboratorio Nacional Of Investigacao Veterinaria | And 2 more authors.
Revista Iberoamericana de Micologia | Year: 2012

Background: Fumonisin B1 (FB1), fumonisin B2 (FB2), and overall mycotoxins feed contamination may cause several effects on crops production and animal health. The contamination occurred predominantly in corn and corn-based foods and feeds. Aims: This survey intends to provide the occurrence of fumonisins in swine and equine mixed feeds in Portugal, making an overview from 2007 to 2010. Methods: A total of 363 samples were analyzed, 258 from swine feed and 105 from horse feed with HPLC method. The detection limit was 50. μg/kg for FB1 and 100. μg/kg for FB2. Results: The overall results were 13% of FB1 occurrence from 2007 to 2010. FB1 was detected in about 17.0% of swine feed samples, being more frequent in 2010 (32.9%). In this year (2010) levels ranged between 66.7 and 3815.5. μg/kg.FB2 occurred only in 2010 in swine feed (6 samples, ranging between 104.0 to 467.2. μg/kg) and in horse feed (1 sample). Conclusions: This represents an increase in occurrence through the analyzed years, but this may not be a threat to animal health, once the values were below the recommended guidance values from European Commission. . © 2011 Revista Iberoamericana de Micología.


PubMed | Ip Laboratorio Nacional Of Investigacao Veterinaria
Type: Journal Article | Journal: Mycotoxin research | Year: 2013

Results of a 2-year (2009-2010) survey on the occurrence of ochratoxin A (OTA) in swine feed and in feed for laying hens in Portugal are reported. A total of 664 samples (478 swine feed, 186 feed for laying hens) were analyzed by a HPLC method using fluorescence detection with 2 g kg(-1) as detection limit. In swine feed, 31 samples (6.49%) were positive for OTA. In feed for laying hens, 12 samples (6.45%) were OTA-positive. The average levels of contamination were low, with median values of positive samples at 3-4 g kg(-1) in both years and both commodities, although a few samples contained exceptionally high levels (maximum 130 g kg(-1)). Only the maximum level sample (swine feed) contained OTA at a concentration exceeding the European Commission guidance value. The remaining OTA concentrations found in feed samples were much lower than the guidance values.


PubMed | Ip Laboratorio Nacional Of Investigacao Veterinaria
Type: Journal Article | Journal: Mycotoxin research | Year: 2013

Mycotoxins are secondary metabolites produced by many genera of fungi in many commodities, under certain conditions. Mycotoxicological control of feed is a procedure that aims to protect human and animal health, avoiding the adverse effects of these undesirable substances. This component of the sanitary control of feed and food is essential to prevent the presence of those substances which can seriously affect the health of the animals. In Portugal, there is relatively few information related to the natural occurrence of mycotoxins in feed. In this context, the authors present results and data compilation concerning the occurrence of mycotoxins in raw materials and also feed for dairy cattle, swine, poultry, horses, fish, laboratory rats and pet; making a generic qualitative appreciation of the risks associated to the presence of mycotoxins in these feedstuffs. The mycotoxins studied: aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 and B2 (FB1, FB2) were analysed by High Performance Liquid Chromatoghraphy (HPLC). Deoxynivalenol (DON) and zearalenone (ZEN) were determined by Thin-Layer Chromatography (TLC). The results suggest that contaminations with these mycotoxins in feed are quite common, revealing the need for surveillance and monitoring programs for the prevention of the sanitary impacts of these non desirable substances.


PubMed | Ip Laboratorio Nacional Of Investigacao Veterinaria
Type: Journal Article | Journal: Revista iberoamericana de micologia | Year: 2012

Fumonisin B1 (FB1), fumonisin B2 (FB2), and overall mycotoxins feed contamination may cause several effects on crops production and animal health. The contamination occurred predominantly in corn and corn-based foods and feeds.This survey intends to provide the occurrence of fumonisins in swine and equine mixed feeds in Portugal, making an overview from 2007 to 2010.A total of 363 samples were analyzed, 258 from swine feed and 105 from horse feed with HPLC method. The detection limit was 50 g/kg for FB1 and 100 g/kg for FB2.The overall results were 13% of FB1 occurrence from 2007 to 2010. FB1 was detected in about 17.0% of swine feed samples, being more frequent in 2010 (32.9%). In this year (2010) levels ranged between 66.7 and 3815.5 g/kg. FB2 occurred only in 2010 in swine feed (6 samples, ranging between 104.0 to 467.2 g/kg) and in horse feed (1 sample).This represents an increase in occurrence through the analyzed years, but this may not be a threat to animal health, once the values were below the recommended guidance values from European Commission.

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