Iovel Kutateladze Institute of Pharmacochemistry

Tbilisi, Georgia

Iovel Kutateladze Institute of Pharmacochemistry

Tbilisi, Georgia
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Perrone A.,University of Salerno | Capasso A.,University of Salerno | Festa M.,University of Salerno | Kemertelidze E.,Iovel Kutateladze Institute of Pharmacochemistry | And 3 more authors.
Fitoterapia | Year: 2012

Two new compounds, a furostanol glycoside (1) and a pregnane glycoside (4), along with eight known compounds, belonging to the classes of spirostane (2,3), pregnane (5-7) and cardenolide (8-10) glycosides, were isolated from the seeds of Digitalis ciliata. Their structures were elucidated by 1D and 2D-NMR experiments as well as ESI-MS analysis. For the first time pregnane glycosides of the diginigenin series have been isolated from D. ciliata. The cytotoxic effects of compounds 1-10 on cell viability of several cancer cell lines, namely human breast cancer (MCF-7), human glioblastoma (T98G), human lung adenocarcinoma (A549), human colon carcinoma (HT-29), and human prostate cancer (PC-3) cell lines were evaluated. Compounds 1, 4, 7 and 8 showed antiproliferative effects against MCF-7, HT-29 and A549 cancer cells with IC 50 values ranging from 8.3 to 20 μM. The effects of compounds 1-10 on cell proliferation were evaluated on these three cancer cell lines by cell cycle analysis of DNA content using flow cytometry. Compounds 7, 8 and 10 induced significant changes in G 2/M cell cycle phase of all analyzed cells. The obtained results indicate that compounds 7, 8 and 10 are cytostatic compounds effective in reducing cell proliferation by inducing accumulation of the cells in the G 2/M phase of the cell cycle. © 2012 Elsevier B.V. All rights reserved.


Tsiklauri L.K.,Iovel Kutateladze Institute of Pharmacochemistry | An G.,State University of New York at Buffalo | Alania M.D.,Iovel Kutateladze Institute of Pharmacochemistry | Kemertelidze E.P.,Iovel Kutateladze Institute of Pharmacochemistry | Morris M.E.,State University of New York at Buffalo
Pharmaceutical Chemistry Journal | Year: 2012

An HPLC method for simultaneous quantitative determination of robinin and its aglycone kaempferol in MCF-7 human breast carcinoma cell line was developed. Robinin and kaempferol were separated using a Platinum EPS C-18 column (4.6 × 250 nm, 5 μm particle size). The detector was set at 380 nm. The mobile phase consisted of solution A [5 % MeOH in phosphate buffer (0.01 M, pH 2.0)] and solution B (THF:i-PrOH:MeOH:H 2O, 150:200:67.5:32.5) in a 60:40 ratio (A:B). Calibration curves for both compounds were linear (R 2 = 0.9997, R 2 = 1) in the concentration range 25 - 1000 ng/mL. The intra- and interday variation coefficients were 1.31 - 8.92 %. The mean recoveries were 97.3 - 104.6 %. Robinin, a flavonoid glycoside isolated from leaves and flowers of Astragalus falcatus, decreases residual nitrogen, creatine, and urea in blood. The finished tablet dosage form prepared from it, flaronin, is used to treat uremia caused by chronic kidney failure. © 2012 Springer Science+Business Media, Inc.


Nebieridze V.,Iovel Kutateladze Institute of Pharmacochemistry | Nebieridze V.,The University of Georgia | Skhirtladze A.,Iovel Kutateladze Institute of Pharmacochemistry | Kemertelidze E.,Iovel Kutateladze Institute of Pharmacochemistry | Ganzera M.,University of Innsbruck
Natural Product Communications | Year: 2017

The structures of four new flavonoid glycosides iV apiotribosides A, B, C and D (1-4, resp.) isolated from the leaves of Tribulus terrestris L. were identified as quercetin 3-O-β]-D-apiofuranosyl-(1.,-2)-[β]-D-apiofuranosyl-(1.,-6)]-β]-D-glucopyranoside (1), quercetin 3-O-β]-D-apiofuranosyl-(1.,-2)-[β-L-rhamnopyranosyl-(1.,-6)]-β]-D-glucopyranoside (2), isorhamnetin 3-O-β]-D-apiofuranosyl-(1.,-2)-[β]-D-apiofuranosyl-(1.,-6)]-β]-D-glucopyranoside (3), and isorhamnetin 3-O-β]-Dapiofuranosyl-( 1.,-2)-[β-L-rhamnopyranosyl-(1.,-6)]-β]-D-glucopyranoside (4).


Skhirtladze A.,Iovel Kutateladze Institute of Pharmacochemistry | Kemertelidze E.,Iovel Kutateladze Institute of Pharmacochemistry | Nebieridze V.,Iovel Kutateladze Institute of Pharmacochemistry | Ganzera M.,University of Innsbruck
Helvetica Chimica Acta | Year: 2016

Three new phenylethanoid glycosides, named digicilisides A - C (1 - 3, resp.), have been isolated from the roots of Digitalis ciliata, along with five known phenylethanoid glycosides. The structures of 1 - 3 were identified as 2-(4-hydroxy-3-methoxyphenyl)ethyl β-d-glucopyranosyl-(1→3)-[α-l-rhamnopyranosyl-(1→6)]-4-O-[(E)-feruloyl]-β-d-glucopyranoside (1), 2-(3,4-dihydroxyphenyl)ethyl α-l-arabinopyranosyl-(1→2)-[β-d-glucopyranosyl-(1→3)]-[α-l-rhamnopyranosyl-(1→6)]-4-O-[(E)-feruloyl]-β-d-glucopyranoside (2), and 2-(3,4-dihydroxyphenyl)ethyl β-d-glucopyranosyl-(1→3)-{6-O-[(E)-feruloyl]-β-d-glucopyranosyl-(1→6)}-4-O-[(E)-caffeoyl]-β-d-glucopyranoside (3). © 2016 Verlag Helvetica Chimica Acta AG, Zürich.


Kemertelidze E.,Iovel Kutateladze Institute of Pharmacochemistry | Skhirtladze A.,Iovel Kutateladze Institute of Pharmacochemistry | Ganzera M.,University of Innsbruck
Helvetica Chimica Acta | Year: 2015

Three new furostanol glycosides, named ciliatasides A, B, and C (1-3, resp.), have been isolated from the roots of Digitalis ciliata, along with two known furostanol glycosides. The structures of the new compounds were identified as (2α,3β,5α,14β,25R)-26-(β-D-glucopyranosyloxy)-2-hydroxyfurost-20(22)-en-3-yl β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-galactopyranoside (1), (2α,3β,5α,14β,22R)-26-(β-D-glucopyranosyloxy)-2-hydroxy-22-methoxyfurost-25(27)-en-3-yl β-D-galac-topyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (2), and (2α,3β,5α,14β,22R, 25R)-26-(β-D-glucopyranosyloxy)-2,22-dihydroxyfurostan-3-yl β-D-glucopyrano-syl-(1 → 2)-[β-D-glucopyranosyl-(1→3)]-β-D-galactopyranoside (3). © 2015 Verlag Helvetica Chimica Acta AG, Zürich.


Skhirtladze A.,Iovel Kutateladze Institute of Pharmacochemistry | Perrone A.,University of Salerno | Montoro P.,University of Salerno | Benidze M.,Iovel Kutateladze Institute of Pharmacochemistry | And 3 more authors.
Phytochemistry | Year: 2011

The occurrence of steroidal saponins in the rhizomes of Yucca gloriosa has been detected by LC-MS. On the basis of the LC-MS analysis, five steroidal glycosides, including three spirostane, one furostane and one cholestane glycosides, along with seven known compounds have been isolated and characterized by ESI-MS and by the extensive use of 1D- and 2D-NMR experiments. Quantitative analysis of the steroidal glycosides in Y. gloriosa rhizomes was performed by an LC-MS method validated according to European Medicines Agency (EMEA) guidelines. The dried BuOH extract obtained from rhizomes contains more than 25% w/w of glycosides, thus Y. gloriosa rhizomes can be considered a rich source of steroidal glycosides. © 2010 Elsevier Ltd. All rights reserved.


Meskheli M.,Tbilisi State Medical University | Bakuridze A.,Tbilisi State Medical University | Vachnadze V.,Iovel Kutateladze Institute of Pharmacochemistry
ICBEE 2010 - 2010 2nd International Conference on Chemical, Biological and Environmental Engineering, Proceedings | Year: 2010

Alkaloids are the natural compounds which mostly contain basic nitrogen atoms. They are characterized by multilateral pharmacological activity, broad spectrum of therapeutic action and hypo toxicity. Receipt of herbal alkaloids is difficult technological process connected with heavy financial expenses. Together with it, there are used organic solvents polluting environment and finished product. The purpose of our research was to develop of alternative and safe technologies of herbal alkaloids receipt. Extraction of alkaloids from medicinal herbal raw material was realized by two methods: with organic solvent and with diluents gas - recirculation of the latter in closed system. The results were justified by HPLC method. © 2010 IEEE.


PubMed | Iovel Kutateladze Institute of Pharmacochemistry
Type: Journal Article | Journal: Phytochemistry | Year: 2010

The occurrence of steroidal saponins in the rhizomes of Yucca gloriosa has been detected by LC-MS. On the basis of the LC-MS analysis, five steroidal glycosides, including three spirostane, one furostane and one cholestane glycosides, along with seven known compounds have been isolated and characterized by ESI-MS and by the extensive use of 1D- and 2D-NMR experiments. Quantitative analysis of the steroidal glycosides in Y. gloriosa rhizomes was performed by an LC-MS method validated according to European Medicines Agency (EMEA) guidelines. The dried BuOH extract obtained from rhizomes contains more than 25% w/w of glycosides, thus Y. gloriosa rhizomes can be considered a rich source of steroidal glycosides.

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