Baaken G.,Albert Ludwigs University of Freiburg |
Baaken G.,Ionera Technologies GmbH i.G. |
Zaitseva E.,Albert Ludwigs University of Freiburg |
Zaitseva E.,Ionera Technologies GmbH i.G. |
And 4 more authors.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | Year: 2013
We here report on a novel method for the automated and highly reliable formation of arrays of suspended lipid membranes of diameters of 6-50 μm that allow high fidelity electrophysiological recordings from embedded ion channels or protein nanopores using voltage clamp (potentiostatic) electronics. The method is adapted to a recently developed microelectrode cavity array (MECA) device and allows reliable automation of an important, hitherto badly-controlled step in the realization of parallel and high-throughput recordings of ionic currents through ion channels and biological nanopores.