INTI Plasticos

Buenos Aires, Argentina

INTI Plasticos

Buenos Aires, Argentina
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Blanco Massani M.,INTI Plasticos | Morando P.J.,Comision Nacional de la Energia Atomica | Morando P.J.,CONICET | Vignolo G.M.,CONICET | Eisenberg P.,INTI Plasticos
Journal of the Science of Food and Agriculture | Year: 2012

Background: Bacteriocins produced by lactic acid bacteria offer enormous promise for food safety preservation. In this study an active multilayer film obtained by the incorporation of lactocin 705 and lactocin AL705, two bacteriocins produced by Lactobacillus curvatus CRL705 with antimicrobial activity against Lactobacillus plantarum CRL691 and Listeria innocua 7, respectively, was characterized for its potential application in active packaging technology. Film activity performance at different storage conditions, bacteriocins transfer into water and sunflower oil, and film surface properties were evaluated. Results: Film activity against L. innocua 7 was maintained during 2, 4 and 6 weeks at 30, 10 and 5 °C respectively. At 30 and 10 °C, activity loss against L. plantarum CRL691 was observed on the second week of storage and after the fourth week at 5 °C. Results showed no significant difference for active multilayer film contact angle and seal properties compared to the control (without bacteriocins). A decrease in lactocin 705 inhibitory activity after sunflower oil contact was observed, while lactocin AL705 remained unaffected. After water contact, film activity was retained for both bacteriocins. Conclusions: As demonstrated by antimicrobial activity and physico-mechanical properties retention, lactocin 705 and AL705 active multilayer film present potential for application in active packaging technology. © 2011 Society of Chemical Industry.


Botana A.,INTI Plasticos | Mollo M.,INTI Plasticos | Eisenberg P.,INTI Plasticos | Torres Sanchez R.M.,INTI Plasticos
Applied Clay Science | Year: 2010

Polymer nanocomposites, based on a bacterial biodegradable thermoplastic polyester, poly(hydroxybutyrate) (PHB), and two commercial montmorillonites (MT), Na-M (MT) and 30B-M (organically modified MT), were prepared by melt-mixing technique at 165 °C. Both clays minerals were characterized by morphology, crystallochemical parameters, and thermal stability. Lower specific surface area (determined by adsorption methods) values were found for 30B-M. The apparent particle size from light scattering measurements, scanning electron microscopy observations, and crystallite size (determined from XRD patterns) of 30B-M indicated a higher degree of particles exfoliation than of Na-M. The nanocomposites PHBNa and PHB30B were characterized by differential scanning calorimetry (DSC), polarized optical microscopy (POM), X-ray diffraction (XRD), transmission electron microscopy (TEM), mechanical properties, and burning behaviour. Intercalation/exfoliation observed by TEM and XRD was more pronounced for PHB30B than PHBNa, indicating the better compatibility of 30B-M with the PHB matrix. An increase in crystallization temperature and a decrease in spherullites size were observed for PHB30B. The intercalation/exfoliation observed by TEM and structure XRD increased the moduli of the nanocomposites. The burning behaviour of PHB30B was influenced by the aggregation of the clay mineral particles. © 2009 Elsevier B.V. All rights reserved.


Sammartino E.K.,INTI Plasticos | Sammartino E.K.,National University of General San Martín | Sammartino E.K.,Laboratorios Rivero | Reboredo M.M.,University of the Sea | Aranguren M.I.,University of the Sea
Journal of Renewable Materials | Year: 2016

Composites made from polypropylene (PP) and local South American fi bers traditionally used in yarnderived craftsmanships, Caranday Palm, were studied regarding the effect of fi ber addition, concentration and characteristics of the coupling agent (molecular weight and percentage of grafted maleic anhydride), as well as type of processing. A laboratory-scale intensive mixing followed by compression, and pilot plant twin extrusion followed by injection, were the two processes investigated. The use of the fi rst one allowed the selection of processable formulations with high fi ber concentration and a percentage of coupling agent below the surface fi ber saturation. In fact, it was found that there is a concentration of maleic anhydride moieties that saturates the surface of the fi ber irrespective of the agent molecular weight. Increasing the concentration of the coupling agent above that value does not produce further improvement of the mechanical properties. The formulations selected from the results of the laboratory process were considered for the pilot plant (extrusioninjection) process. The use of the second process consisting of extrusion-injection molding showed that the high shear developed in this process leads to defi brillation of the fi ber bundles. In the case of the Caranday Palm fi bers, this defi brillation resulted in elemental fi bers (micron size) being the effective reinforcement of the composite instead of the technical fi bers (millimeter size) that were present in the composites prepared by compression. Better mechanical properties were achieved by using the latter method. © 2016 Scrivener Publishing LLC.


Blanco Massani M.,INTI Plasticos | Botana A.,INTI Plasticos | Botana A.,Instituto 3iA UNSAM | Eisenberg P.,INTI Plasticos | And 2 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2014

Antimicrobial wheat gluten film was obtained at pilot scale by Lactobacillus curvatus CRL705 bacteriocins inclusion in the film-forming solution. Bacteriocins' minimum inhibitory concentration for the film activation was 2133 AU cm-3 (lactocin AL705) and 267 AU cm-3 (lactocin 705). Mechanical and barrier properties as well as film ageing kinetics were not significantly affected by the addition of bacteriocins. The antimicrobial film performance during ageing was assessed. Film activity against Listeria innocua 7 and Lactobacillus plantarum CRL691 was observed over 50 days of ageing. Even when the release of bacteriocins from the film upon water contact was observed for both bacteriocins at the beginning of the ageing period, and anti-Listeria activity was delivered to the simulant up to the 15th day of ageing, film residual activity for both bacteriocins was observed over 50 days. The results confirm the potential of a gluten film doped with L. curvatus CRL705 bacteriocins as a carrier of bacteriocins to avoid Listeria and lactic acid bacterial growth, thus enhancing quality and safety in foods. © 2014 Taylor & Francis.


Massani M.B.,INTI Plasticos | Vignolo G.M.,CONICET | Eisenberg P.,INTI Plasticos | Morando P.J.,CONICET | And 2 more authors.
LWT - Food Science and Technology | Year: 2013

Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705α and lac705β) and bacteriocin/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 μg cm-2 and a minimum inhibition concentration of 1 μg ml-1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705β and lac705α peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems. © 2013 Elsevier Ltd.


PubMed | INTI Plasticos
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2012

Bacteriocins produced by lactic acid bacteria offer enormous promise for food safety preservation. In this study an active multilayer film obtained by the incorporation of lactocin 705 and lactocin AL705, two bacteriocins produced by Lactobacillus curvatus CRL705 with antimicrobial activity against Lactobacillus plantarum CRL691 and Listeria innocua 7, respectively, was characterized for its potential application in active packaging technology. Film activity performance at different storage conditions, bacteriocins transfer into water and sunflower oil, and film surface properties were evaluated.Film activity against L. innocua 7 was maintained during 2, 4 and 6 weeks at 30, 10 and 5 C respectively. At 30 and 10 C, activity loss against L. plantarum CRL691 was observed on the second week of storage and after the fourth week at 5 C. Results showed no significant difference for active multilayer film contact angle and seal properties compared to the control (without bacteriocins). A decrease in lactocin 705 inhibitory activity after sunflower oil contact was observed, while lactocin AL705 remained unaffected. After water contact, film activity was retained for both bacteriocins.As demonstrated by antimicrobial activity and physico-mechanical properties retention, lactocin 705 and AL705 active multilayer film present potential for application in active packaging technology.

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