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Abramov V.,Institute of Immunological Engineering | Khlebnikov V.,Institute of Immunological Engineering | Kosarev I.,Institute of Immunological Engineering | Bairamova G.,Research Center for Obstetrics | And 10 more authors.
Probiotics and Antimicrobial Proteins | Year: 2014

Lactobacillus crispatus 2029 isolated upon investigation of vaginal lactobacilli of healthy women of reproductive age was selected as a probiotic candidate. The aim of the present study was elucidation of the role of L. crispatus 2029 in resistance of the female reproductive tract to genitourinary pathogens using cervicovaginal epithelial model. Lactobacilluscrispatus 2029 has surface layers (S-layers), which completely surround cells as the outermost component of their envelope. S-layers are responsible for the adhesion of lactobacilli on the surface of cervicovaginal epithelial cells. Study of interactions between L. crispatus 2029 and a type IV collagen, a major molecular component of epithelial cell extracellular matrix, showed that 125I-labeled type IV collagen binds to lactobacilli with high affinity (Kd = (8.0 ± 0.7) × 10−10 M). Lactobacilluscrispatus 2029 consistently colonized epithelial cells. There were no toxicity, epithelial damage and apoptosis after 24 h of colonization. Electronic microscope images demonstrated intimate association between L. crispatus 2029 and epithelial cells. Upon binding to epithelial cells, lactobacilli were recognized by toll-like 2/6 receptors. Lactobacilluscrispatus induced NF-κB activation in epithelial cells and did not induce expression of innate immunity mediators IL-8, IL-1β, IL-1α and TNF-α. Lactobacilluscrispatus 2029 inhibited IL-8 production in epithelial cells induced by MALP-2 and increased production of anti-inflammatory cytokine IL-6, maintaining the homeostasis of female reproductive tract. Lactobacilluscrispatus 2029 produced H2O2 and provided wide spectrum of antagonistic activity increasing colonization resistance to urinary tract infections by bacterial vaginosis and vulvovaginal candidiasis associated agents. © 2014, Springer Science+Business Media New York.


Kiryukhina N.V.,Institute of Biomedical Problems | Melnikov V.G.,International Science and Technology Center | Suvorov A.V.,Institute of Biomedical Problems | Morozova Y.A.,Institute of Biomedical Problems | Ilyin V.K.,Institute of Biomedical Problems
Probiotics and Antimicrobial Proteins | Year: 2013

Effectiveness of saline suspension of Corynebacterium pseudodiphtheriticum containing spray was assessed in a 30-days chamber experiment modeling the effects of hyperthermia and polluted air on humans. Spray was targeted at eliminating Staphylococcus aureus from the nasal cavity of human subjects. Three of four volunteers became S. aureus carriers in the course of the experiment, and one was a chronic carrier of methicillin-resistant S. aureus (MRSA). Spray application eradicated S. aureus in three subjects and reduced its presence in the MRSA carrier. Results of the study suggest that C. pseudodiphtheriticum can be used for control of S. aureus in the nasal environment. However, further investigations are required. © 2013 Springer Science+Business Media New York.


Zaporozhets T.S.,Russian Academy of Medical Sciences | Besednova N.N.,Russian Academy of Medical Sciences | Kuznetsova T.A.,Russian Academy of Medical Sciences | Zvyagintseva T.N.,RAS Pacific Institute of Bioorganic Chemistry | And 3 more authors.
Russian Journal of Marine Biology | Year: 2014

Based on our own and current literature data, we analyzed the prebiotic properties of polysaccharides and extracts of seaweeds. The role of prebiotics, particularly polysaccharides, in the normalization of intestinal microflora is discussed; consideration is also given to the possibility of seaweed polysaccharide fermentation by colonic microflora and selective stimulation of the growth of colonic bifidobacteria. This review also analyzes other useful properties of seaweed polysaccharides and discusses the prospects for inclusion of seaweed polysaccharides in the composition of functional nutrition products for the purposes of correcting intestinal bacterial disorders and inflammatory processes and normalizing the immune and metabolic status. © 2014 Pleiades Publishing, Ltd.


Buganov O.V.,B. I. Stepanov Institute of Physics | Grabtchiko A.S.,B. I. Stepanov Institute of Physics | Malakhov Y.I.,International Science and Technology Center | Popov Y.M.,RAS Lebedev Physical Institute | And 2 more authors.
Laser Physics Letters | Year: 2012

Measurements of Raman amplification in KGd(WO4)2 (KGW) and barium nitrate crystals at femtosecond excitation demonstrate spectral transformation of amplification band with change of pump parameters. The halfheight amplification bandwidth of up to 45 nm (650 cm-1) what is 5 times larger than the pumping pulse spectral band 8.5 nm (130 cm-1) was observed for KGW crystal. Implementation of impulsive excitation for the low-frequency vibrations allows estimations of the dephasing times and linewidths for the 87 and 83 cm-1 Raman lines in KGW and barium nitrate. © 2012 by Astro, Ltd.


Orlovich V.A.,B. I. Stepanov Institute of Physics | Malakhov Yu. I. Y.I.,International Science and Technology Center | Popov Yu. M. Y.M.,RAS Lebedev Physical Institute | Busko D.N.,B. I. Stepanov Institute of Physics | And 4 more authors.
Laser Physics Letters | Year: 2012

The processes of transient Raman generation and Raman amplification in KGd(WO4)2 (KGW) crystals pumped by laser pulses with the width of about 150 fs were studied experimentally. For excitation, Bessel beams were used. Gaussian beams of Stokes and laser radiation were used at amplification stage. Raman shifts of 768 or 901 cm-1 were obtained depending on orientation of KGW crystals. For optimal conditions the gain was more than 100. The conversion efficiency of amplifying (pump) radiation into amplified Stokes radiation reached 50 %. © 2012 by Astro, Ltd.


Kirillov V.A.,RAS Boreskov Institute of Catalysis | Kuzin N.A.,RAS Boreskov Institute of Catalysis | Kireenkov V.V.,RAS Boreskov Institute of Catalysis | Amosov Yu.I.,RAS Boreskov Institute of Catalysis | And 4 more authors.
Theoretical Foundations of Chemical Engineering | Year: 2011

A method for the production of a hydrogen-rich gas on board a vehicle was suggested and driving- and bench-tested for application in studies on energy-efficient internal combustion engines with minimum CO, CO2, CH, and NO x emissions. The generated gas is further added to the main fuel fed to the engine. Catalysts for hydrocarbon fuel conversion to syngas were developed. A compact on-board syngas generator mounted under the motor hood and a generator control system adapted to the engine control system were designed. It was shown experimentally that the suggested solution allows a reduction of 13-40% in the fuel rate depending on the operating mode under the urban cycle conditions and considerably decreases the release of CO, CO 2, and NO x . Prospects for the applications of this technology for creating ecologically clean engines were assessed. © 2011 Pleiades Publishing, Ltd.


Kulikov E.G.,National Research Nuclear University MEPhI | Shmelev A.N.,National Research Nuclear University MEPhI | Apse V.A.,National Research Nuclear University MEPhI | Kulikov G.G.,International Science and Technology Center
International Conference on the Physics of Reactors 2010, PHYSOR 2010 | Year: 2010

A calculational model is presented for evaluating fissionable materials protection against unauthorized proliferation. The model takes into consideration the main nuclear physics and thermal processes in a crude (hypothetical) implosion-type nuclear explosive device (NED). It is proposed that plutonium is used as fissionable material in implosion-type NED. To protect fissionable material (plutonium) it is suggested to add some amount of radioactive isotope (238Pu), the alpha-decay heat of which could overheat implosion-type NED and render it non-functional. A mathematical model of non-stationary (time-dependent) warm-up of implosion-type NED has been developed in order to evaluate the rate of loss of its effectiveness for different isotopic compositions of plutonium and for different methods of heat removal. The importance of measures for slowing down warm-up process of implosion-type NED was demonstrated. It was discovered that the time, during which implosion-type NED is functional, is a decisive factor in terms of evaluation of plutonium proliferation protection.


Tocheny L.V.,International Science and Technology Center | Gudowski W.,International Science and Technology Center
International Congress on Advances in Nuclear Power Plants 2010, ICAPP 2010 | Year: 2010

The ISTC is a unique international organization created in Moscow in 1994 by Russia, USA, EU and Japan. Later Republic of Korea and Canada, and several CIS countries acceded to ISTC. The basic idea behind establishing the ISTC was to support non-proliferation of the mass destruction weapons technologies by re-directing former Soviet weapons scientists to peaceful research thus preventing the drain of dangerous knowledge and expertise from Russia and other CIS countries. Presently, the ISTC now has 39 member countries (27 from EU), representing the CIS, Europe, Asia, and North America. The Partner list includes over 200 organizations and leading industrial companies from all ISTC parties. Numerous science and technology projects were realized with the ISTC support in different areas, from biotechnologies and environmental problems to all aspects of nuclear studies, including those focused on the development of effective innovative concepts and technologies in the nuclear field, in general, and for improvement of nuclear safety, in particular.


PubMed | International Science and Technology Center and Kingston University
Type: Journal Article | Journal: Genome announcements | Year: 2014

In this report, we present a draft sequence of Bacillus subtilis KATMIRA1933. Previous studies demonstrated probiotic properties of this strain partially attributed to production of an antibacterial compound, subtilosin. Comparative analysis of this strains genome with that of a commercial probiotic strain, B.subtilis Natto, is presented.


PubMed | International Science and Technology Center, Southern Federal University and Kingston University
Type: Journal Article | Journal: Genome announcements | Year: 2014

In this report, we present a draft genome sequence of Bacillus amyloliquefaciens strain B-1895. Comparison with the genome of a reference strain demonstrated similar overall organization, as well as differences involving large gene clusters.

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