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Zaporozhets T.S.,Russian Academy of Medical Sciences | Besednova N.N.,Russian Academy of Medical Sciences | Kuznetsova T.A.,Russian Academy of Medical Sciences | Zvyagintseva T.N.,RAS Pacific Institute of Bioorganic Chemistry | And 3 more authors.
Russian Journal of Marine Biology | Year: 2014

Based on our own and current literature data, we analyzed the prebiotic properties of polysaccharides and extracts of seaweeds. The role of prebiotics, particularly polysaccharides, in the normalization of intestinal microflora is discussed; consideration is also given to the possibility of seaweed polysaccharide fermentation by colonic microflora and selective stimulation of the growth of colonic bifidobacteria. This review also analyzes other useful properties of seaweed polysaccharides and discusses the prospects for inclusion of seaweed polysaccharides in the composition of functional nutrition products for the purposes of correcting intestinal bacterial disorders and inflammatory processes and normalizing the immune and metabolic status. © 2014 Pleiades Publishing, Ltd.

Ushakova N.A.,RAS Severtsov Institute of Ecology | Abramov V.M.,Institute of Immunological Engineering | Khlebnikov V.S.,Institute of Immunological Engineering | Semenov A.M.,Moscow State University | And 8 more authors.
Probiotics and Antimicrobial Proteins | Year: 2012

The biofilm formation took place in 48 h within the solid substrate cultivation of Lactobacillus plantarum 8-RA-3 strain on the wheat bran saturated with the MRS medium. The drying of the bran fermented by lactobacilli resulted in a decrease in the number of colony-forming units (CFU) from 23. 0 × 10 8 to 6. 9 × 10 5 CFU/g in daily samples and to less than 10 4 CFU/g in 2- and 3-day samples. However, according to the fluorescence-based live/dead assay data, more than 40 % of the non-cultured bacteria were viable. As a result of mice kept on a diet with the introduction of bran fermented by Lact. plantarum 8-RA-3 for 72 h into the fodder, a recovery of normal level of intestinal lactobacilli, inhibited by administration of antibiotic was noted. The strain genetically identical to the Lact. plantarum 8-RA-3 was isolated from the feces of these mice. The results indicate that solid substrate cultivated Lact. plantarum 8-RA-3 strain formed a biofilm. Once dried and transferred into a non-cultured state, biofilm cells retained its viability and biological activity. © 2012 Springer Science + Business Media, LLC.

Kiryukhina N.V.,Institute of Biomedical Problems | Melnikov V.G.,International Science and Technology Center | Suvorov A.V.,Institute of Biomedical Problems | Morozova Y.A.,Institute of Biomedical Problems | Ilyin V.K.,Institute of Biomedical Problems
Probiotics and Antimicrobial Proteins | Year: 2013

Effectiveness of saline suspension of Corynebacterium pseudodiphtheriticum containing spray was assessed in a 30-days chamber experiment modeling the effects of hyperthermia and polluted air on humans. Spray was targeted at eliminating Staphylococcus aureus from the nasal cavity of human subjects. Three of four volunteers became S. aureus carriers in the course of the experiment, and one was a chronic carrier of methicillin-resistant S. aureus (MRSA). Spray application eradicated S. aureus in three subjects and reduced its presence in the MRSA carrier. Results of the study suggest that C. pseudodiphtheriticum can be used for control of S. aureus in the nasal environment. However, further investigations are required. © 2013 Springer Science+Business Media New York.

Buganov O.V.,B. I. Stepanov Institute of Physics | Grabtchiko A.S.,B. I. Stepanov Institute of Physics | Malakhov Y.I.,International Science and Technology Center | Popov Y.M.,RAS Lebedev Physical Institute | And 2 more authors.
Laser Physics Letters | Year: 2012

Measurements of Raman amplification in KGd(WO4)2 (KGW) and barium nitrate crystals at femtosecond excitation demonstrate spectral transformation of amplification band with change of pump parameters. The halfheight amplification bandwidth of up to 45 nm (650 cm-1) what is 5 times larger than the pumping pulse spectral band 8.5 nm (130 cm-1) was observed for KGW crystal. Implementation of impulsive excitation for the low-frequency vibrations allows estimations of the dephasing times and linewidths for the 87 and 83 cm-1 Raman lines in KGW and barium nitrate. © 2012 by Astro, Ltd.

Orlovich V.A.,B. I. Stepanov Institute of Physics | Malakhov Yu. I. Y.I.,International Science and Technology Center | Popov Yu. M. Y.M.,RAS Lebedev Physical Institute | Busko D.N.,B. I. Stepanov Institute of Physics | And 4 more authors.
Laser Physics Letters | Year: 2012

The processes of transient Raman generation and Raman amplification in KGd(WO4)2 (KGW) crystals pumped by laser pulses with the width of about 150 fs were studied experimentally. For excitation, Bessel beams were used. Gaussian beams of Stokes and laser radiation were used at amplification stage. Raman shifts of 768 or 901 cm-1 were obtained depending on orientation of KGW crystals. For optimal conditions the gain was more than 100. The conversion efficiency of amplifying (pump) radiation into amplified Stokes radiation reached 50 %. © 2012 by Astro, Ltd.

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