International Joint Academy of Biotechnology and Medicine

Tianjin, China

International Joint Academy of Biotechnology and Medicine

Tianjin, China
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Wu S.,Tianjin University | Gao W.,Tianjin University | Qiu F.,Peking Union Medical College | Man S.,Tianjin University | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012

Polyphyllin D and Paris H are two potential antitumor active components in Paris polyphylla, one of the Traditional Chinese Medicines (TCMs). The present study details the development and validation of a rapid, sensitive and accurate LC-ESI-MS/MS method for the separation and simultaneous determination of Polyphyllin D and Paris H in rat plasma using Ginsenoside Rh2 as the internal standard (IS). A simple protein precipitation method was used for the preparation of plasma sample. Chromatographic separation was successfully achieved on an Agilent Zorbax C18 column using a step gradient program with the mobile phase of 10mmol/L aqueous ammonium acetate and acetonitrile. Both analytes were detected by negative mode electrospray ionization mass spectrometry. Selected reaction monitoring (SRM) was applied for all monitored analytes. This method demonstrated good linearity and did not show any endogenous interference. The lower limits of quantification (LLOQs) of Polyphyllin D and Paris H were both 1.0ng/mL using 100μL rat plasma. The average recoveries of Polyphyllin D and Paris H from rat plasma were both above 80%. The inter-day precisions (%RSD) of both analytes determined in five days were all below 15%. The developed and validated method has been successfully applied in the simultaneous quantification and pharmacokinetic studies of Polyphyllin D and Paris H in rats. © 2012 .


Qiu F.,Peking Union Medical College | Zhou S.,Peking Union Medical College | Zhou S.,Jilin Agricultural University | Fu S.,International Joint Academy of Biotechnology and Medicine | And 3 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

A sensitive and accurate LC-ESI-MS/MS method was developed and validated of for the determination of 6'-hydroxy justicidin A (HJA), a potential antitumor active component isolated from Justicia procumbens in rat plasma using a simple liquid-liquid extraction (LLE) method for sample preparation. Chromatographic separation was achieved on an Agilent Zorbax-C18 column (2.1mm×50mm, 3.5μm) using a step gradient program with the mobile phase of 0.1% formic acid aqueous solution and acetonitrile with 0.1% formic acid. HJA and IS (buspirone) were detected using electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. This method demonstrated good linearity and did not show any endogenous interference with the active compound and IS peaks. The lower limit of quantification (LLOQ) of HJA was 0.50ng/ml in 50μl rat plasma. The developed and validated method has been successfully applied to the quantification and pharmacokinetic study of HJA in rats after intravenous and oral administration of 0.25mg/kg HJA. The oral bioavailability (F) of HJA was estimated to be 36.0±13.4% with an elimination half-life (t1/2) value of 1.04±0.20h. © 2012 Elsevier B.V.


Wang Y.,Tianjin University of Traditional Chinese Medicine | Xu C.,Tsinghua University | Wang P.,Tianjin University of Traditional Chinese Medicine | Lin X.,International Joint Academy of Biotechnology and Medicine | And 5 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

A specific HPLC-MS/MS method was developed and validated for simultaneous determination of ten constituents including albiflorin, oxypaeoniflorin, paeoniflorin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, ononin, glycyrrhizin and glycyrrhetinic acid in rat plasma using genistein as an internal standard (IS). The rat plasma samples were prepared by a one-step direct protein precipitation procedure with methanol. HPLC separation was achieved on a Zorbax XDB-C18 column (2.1mm×50mm i.d., 3.5μm) with gradient elution (A: 0.1% aqueous formic acid; B: methanol with 0.1% formic acid) at a flow rate of 0.5mL/min in a run time of 7min. All analytes and IS were detected by multiple reaction monitoring scanning with electrospray ionization in the negative ion mode. Calibration curves showed good linearity (r>0.998) over a wide concentration range for all analytes. The intra- and inter-day precisions were all within 15% and the accuracies were in the range of -6.2% to 10.1%. The validated method was successfully applied to determination and comparative pharmacokinetics investigation of the ten constituents in rat plasma after oral administration of different combinations (Radix Paeoniae Alba:Glycyrrhiza uralensis=1:1 or 4:1) of Shaoyao-Gancao-Decoction (SGD) extracts. Pharmacokinetic parameters were evaluated by a compartment model. There were perceptible differences in pharmacokinetic parameters (Cmax, AUC0-t, CL) of the analytes except for liquiritin between the two groups of SGD. © 2013 Elsevier B.V.


Qiu F.,Peking Union Medical College | Fu S.,International Joint Academy of Biotechnology and Medicine | Zhou S.,Peking Union Medical College | Zhou S.,Jilin Agricultural University | And 2 more authors.
Biomedical Chromatography | Year: 2013

This study firstly describes the development of an accurate and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for the quantification of Taiwanin E methyl ether (TEME) in rat plasma. The assay involved a simple liquid-liquid extraction step with ethyl acetate and a gradient elution using a mobile phase consisting of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid. Chromatographic separation was successfully achieved on an Agilent Zorbax-C18 column (2.1×50 mm, 3.5 μm) with a flow rate of 0.40 mL/min. The multiple reaction monitoring was based on the transitions of m/z=379.1→320.1 for TEME and 386.1→122.0 for buspirone (internal standard). The assay was validated to demonstrate the specificity, linearity, recovery, accuracy, precision and stability. The lower limit of quantification was 0.50 ng/mL in 50 μL of rat plasma. The developed and validated method was successfully applied to the quantification and pharmacokinetic study of TEME in rats after intravenous and oral administration of 1.45 mg/kg TEME. The oral absolute bioavailability of TEME was estimated to be 5.85±1.41% with an elimination half-life value of 2.61±0.55 h, suggesting its poor absorption and/or strong metabolism in vivo. © 2012 John Wiley & Sons, Ltd.

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