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Francis A.P.,Anna University | Murthy P.B.,International Institute of Biotechnology and Toxicology IIBAT | Devasena T.,Anna University
Journal of Nanoscience and Nanotechnology | Year: 2014

We have optimized a protocol for the preparation of bisdemethoxy curcumin analog nanoparticles (BDMCA-NP) by the solvent assisted process. The structural similarities between bulk and nano BDMCA were determined by Co-TLC, NMR and FTIR. This shows that our synthesis protocol enhanced the dispersibility and reduce the size of BDMCA without altering the integrity of functional moieties and structure, which is crucial for anticancer and antioxidant activities. The morphology and size of BDMCA-NP as determined by SEM, HRTEM and DLS was found to be around 80 nm. BDMCA-NP treated breast cancer cell lines (MCF 7) showed cell death as characterized by MTT assay. Flow cytometric analysis of BDMCA-NP treated MCF 7 cell lines showed an increase of cell count in G2/M phase indicates the cell cycle arrest. Western blot analysis revealed the presence of caspase 3, caspase 9, cleaved fragments of PARP and Bax proteins in the BDMCA-NP treated MCF 7 cell lines, but not in untreated cell lines. To recap, we have prepared BDMCA-NP by solvent assisted process, which exerted anticancer activity against breast cancer cells, which may be due to (i) enhanced dispersibility and surface: volume ratio, (ii) apoptosis (iii) mitochondrial pathway induced cell death, (iv) G2/M phase cell cycle arrest and (v) disassembly of mitotic spindle of the cancer cells. Thus, nano BDMCA can be used as a potent anticancer agent. Copyright © 2014 American Scientific Publishers All rights reserved.

Parvathi M.V.S.,International Institute of Biotechnology and Toxicology IIBAT | Murthy P.B.,International Institute of Biotechnology and Toxicology IIBAT | Vennila M.,Salem College
Tumor Biology | Year: 2013

BMI1 is the first functional mammalian Polycomb group (PcG) proto-oncogene involved in multiple biological processes. Regulation of B cell-specific Moloney murine leukaemia virus integration site 1 (BMI1) expression with increase in histological grades of breast carcinoma in correlation with hormone receptor status was studied in 60 Indian breast cancer patient's formalin-fixed paraffin-embedded tissue blocks. Relative expression of BMI1 was studied using real-time PCR. Immunohistochemistry explained the distribution of hormone receptor markers. Correlation of BMI1 gene expression with oestrogen receptor, progesterone receptor (PR) and human epidermal growth factor receptor 2/neu status was analysed using Hex - protein docking tool. The hormone receptor expression was reduced with increasing grades of breast tumour. BMI1 gene expression was downregulated (real-time polymerase chain reaction analysis). Docking analysis explained the correlation between BMI1 and PR expression. BMI1 gene was co-regulated (down) with PR in the invasive ductal breast carcinoma with relative progression explicating it a diagnostic biomarker for ductal carcinoma of the breast. © 2013 International Society of Oncology and BioMarkers (ISOBM).

Chirukuri R.,International Institute of Biotechnology and Toxicology IIBAT | Atmakuru R.,International Institute of Biotechnology and Toxicology IIBAT
Chemosphere | Year: 2015

The dissipation kinetics and the adsorption characteristics of bispyribac sodium, a pyrimidinyloxybenzoic herbicide, in 21 types of soil collected from different locations in the U.S., Italy, Spain, Greece, France, U.K., the Netherlands, Germany, and India were evaluated under laboratory conditions. The soil sorption study was conducted using the batch equilibrium process. The paper also investigated the adsorption efficiency of bispyribac sodium in the presence of different kinds of background electrolytes, surfactants, and different temperatures in two different soils. The results showed that the Freundlich equation fits its adsorption well, and the Freundlich adsorption constant values (Kf) ranged from 0.3 to 5.6mLg-1. Adsorption isotherms were nonlinear, with 1/nf values <1. Bispyribac sodium adsorption by two soils increased with increasing electrolytes concentration using CaCl2, KCl, NH4Cl, KH2PO4 and MgCl2 as a background electrolytes. The adsorption coefficient value decreased when anionic and nonionic surfactants were used at the three surfactant concentrations in two types of soil but increased with cationic surfactant, and temperature. Sorption was positively correlated with OM and negatively correlated with a soil pH of 5.0 to 8.1. The free energy (δG) values of bispyribac sodium in the soils were less than 40kJmol-1 and negative values were obtained. This indicates that the adsorption of bispyribac sodium is mainly a physical and spontaneous process. The GUS values were less than 2.9 in all the soil types studied, and the residues of bispyribac sodium were low to moderate to leacher (mobile) in the soil. © 2014 Elsevier Ltd.

Thiripura Sundari M.,International Institute of Biotechnology and Toxicology IIBAT | Ramesh A.,International Institute of Biotechnology and Toxicology IIBAT
Carbohydrate Polymers | Year: 2012

The cellulose nanofibers from the water blocking aquatic weed - water hyacinth was successfully prepared. The crude and pure cellulose microfibers were initially obtained from the weed plant by following chemical treatments such as bleaching, alkaline and sodium chlorite reactions. The micron-sized fibers obtained from the stems were cryocrushed with liquid nitrogen to release the bundles of nanofibers and followed the sonication for individualization of fibers. The treated fibers were screened through Fourier Transform Infrared Spectroscopy (FTIR) to confirm the removal of impurities from the fibers. The surface morphology of the aqueous suspension before cryocrushing and after the sonication process was investigated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Thermal stability of the fiber was increased after chemical treatment; this was confirmed by thermogravimetric analysis (TGA). The synthesized nanofibers were in the diameter range of 20-100 nm from the SEM and 25 nm from the TEM analysis. © 2011 Published by Elsevier Ltd. All rights reserved.

Murkunde Y.V.,International Institute of Biotechnology and Toxicology IIBAT | Murthy P.B.,International Institute of Biotechnology and Toxicology IIBAT
International Journal of Toxicology | Year: 2010

Brassinosteroids (BRs) are close analogues of animal cholesterol. Brassinosteroids have shown their great value as yield promoters of a variety of plants. In view of its steroidal moiety and recent use in agriculture in many countries, the teratogenic potential of homobrassinolide (HBR) was evaluated in Wistar rats. Homobrassinolide was administered by oral gavage at doses 0, 100, and 1000 mg/kg body weight in water during gestation days (GD) 6 to 15 in groups of 20 mated females. Maternal and embryo-fetal toxicity was analyzed by studying the effects such as clinical signs, mortality/morbidity, abortions, body weight, feed consumption, and pregnancy data, gravid uterine weights, implantation losses, litter size, external, visceral, and skeletal malformations. No treatment-related effect was observed on any of the maternal/fetal end points in any dose group. From the results, it can be concluded that HBR is nonteratogenic at doses as high as up to 1000 mg/kg body weight in Wistar rats. © The Author(s) 2010.

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