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Nageswara Rao T.,International Inisitituate of Biotechnology and Toxicology IIBAT | Sreenivasulu D.,Orchid Chemicals and Pharmaceuticals Ltd | Satish P.U.V.,International Inisitituate of Biotechnology and Toxicology IIBAT | Parvathamma T.,International Inisitituate of Biotechnology and Toxicology IIBAT
International Journal of Pharma and Bio Sciences | Year: 2012

A simple, sensitive and specific reverse phase high performance liquid chromatographic method has been developed for the determination of granisetron hydrochloride in injectable dosage forms. Chromatographic separation was achieved on a peerless basic C18 (250×4.6mm), 5.0 'm column with a diluted 1.6 ml of ortho phosphoric acid in 800 ml water, added 200ml acetonitrile, added 1.0ml of Hexylamine and mixed. Then adjusted the pH to 7.5 with triethylamine as mobile phase, detection was at 304nm. Response was a linear function of concentration in the range 5-0.02mg/L for granisetron hydrochloride. LOD and LOQ for granisetron hydrochloride were found 0.01mg/L and 0.03mg/L. Accuracy (recoveries 94-97%) and reproducibility were found to satisfactory. Source


Parvathamma T.,International Inisitituate of Biotechnology and Toxicology IIBAT | Ramesh A.,International Inisitituate of Biotechnology and Toxicology IIBAT | Nageswara Rao T.,International Inisitituate of Biotechnology and Toxicology IIBAT
International Journal of ChemTech Research | Year: 2012

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of paclobutrazol in mango fruite. The evaluated parameters included the type and amount of sorbent (silica gel and Alumina) and the nature of eluent (Tetrahydrofuron, Acetonitrile and MilliQ water).The best results were obtained using 1.0 g of mango fruit sample, 1.0 g of silica gel as sorbet and 20ml of Tetrahydrofuron - Acetonitrile - MilliQ water (1:1:1), (v/v)).The method was validated using mango fruit samples spiked with paclobutrazol at different concentration levels (0.03 and 0.3 μg/mL). Average recoveries (using each concentration six replicates) ranged 89-93%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-2.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 μg/mL and 0.03 μg/mL respectively. Source

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