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A rapid and simple quantitative method for the simultaneous determination of the four aflatoxins (AFs) B1, B2, G1 and G2 was developed using reverse homogeneous liquid-liquid extraction (RHLEE) and HPLC postcolumn derivatization-fluorescence (HPLC-FL) detection. The method based on the rapid extraction of AFs from a methanolic sample into chloroform after addition of water containing KBr, with a method we called reverse homogeneous liquid-liquid extraction. Recoveries were in the range of 88-125% and the limits of detection were between 0.001-0.042 ng g-1 for different AFs. Wheat and pistachio were chosen for the analysis of real samples and the method was also successfully applied to a FAPAS® TEST MATERIAL (T04151). © Springer-Verlag 2011.

Sheijooni-Fumani N.,International Goods Inspection Company | Hassan J.,International Goods Inspection Company | Yousefi S.R.,International Goods Inspection Company
Journal of Separation Science | Year: 2011

A simple and rapid method based on homogeneous liquid-liquid extraction coupled to HPLC with fluorescence detection was developed for the determination of aflatoxin B1 (AFB1) in the rice and grain samples after post-column derivatization. The proposed method eliminated the use of immunoaffinity columns for clean-up in the determination of AFB1. The parameters affecting recovery and preconcentration such as type and volume of organic solvent, volume ratio of water/methanol, concentration of phase separator reagent and extraction time were optimized. Under the optimized conditions, the calibration graph was linear in the concentration range of 0.01-1.0 ng/g with the detection limit of 0.003 ng/g. This method was successfully applied for the analysis of AFB1 in different cereal samples. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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