International Center for Cardio Thoracic and Vascular Diseases

Chennai, India

International Center for Cardio Thoracic and Vascular Diseases

Chennai, India
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Ramesh B.,International Center for Cardio Thoracic and Vascular Diseases | Bishi D.K.,International Center for Cardio Thoracic and Vascular Diseases | Rallapalli S.,International Center for Cardio Thoracic and Vascular Diseases | Arumugam S.,MIOT Hospital | And 2 more authors.
Cytotechnology | Year: 2012

Mesenchymal stem cells (MSCs) are multipotent, can be easily expanded in culture and hence are an attractive therapeutic tool for cardiac repair. MSCs have tremendous potential to transdifferentiate to cardiac lineage both in vitro and in vivo. The present study examined the differentiation capacity of conditioned media derived from ischemic cardiac tissue on human MSCs. Human Bone marrow- derived MSCs after due characterization by immunocytochemistry and flow cytometry for MSC specific markers were induced by culture media derived from ischemic (n = 13) and non-ischemic (n = 18) human cardiac tissue. Parallel cultures were treated with 5-azacytidine (5-azaC), a potent cardiomyogen. MSCs induced with ischemic conditioned media formed myotube like structures, expressed sarcomeric Troponin I, alpha myosin heavy chain proteins and were positive for cardiac specific markers (Nkx2.5, human atrial natriuretic peptide, myosin light chain-2a, GATA-4) as was observed in 5-azaC treated cells. However, uninduced MSCs as well as those induced with non-ischemic cardiac conditioned media still maintained the fibroblast morphology even after 3 weeks post-induction. Transmission electron microscopic studies of cardiomyocyte-like cells derived from MSCs revealed presence of sarcomeric bands but failed to show gap junctions and intercalated discs as of adult cardiomyocytes. These findings demonstrate that ischemic cardiac conditioned media induces morphological and molecular changes in MSCs with cardiac features, but at a primitive stage. Proteomics analysis of the ischemic conditioned media revealed differential expression of three relevant proteins (C-type lectin superfamily member 13, Testis-specific chromodomain protein Y2 and ADP/ATP translocase 1), whose exact role in cardiac regeneration needs further analysis. © Springer Science+Business Media B.V. 2012.


Vijayalakshmi R.,SRM University | Nayar S.,International Center for Cardio Thoracic and Vascular Diseases | Cherian K.M.,International Center for Cardio Thoracic and Vascular Diseases
American Journal of Infectious Diseases | Year: 2010

Problem statement: The primary aim was to study the expression of CD44 protein in the heart valves removed surgically for either stenosis or regurgitation and to study the morphology of valves using histochemical staining. Approach: We studied 107 valves which were collected from the International Centre for Cardio Thoracic and Vascular Diseases. Results: Aortic and mitral valves were obtained from the centre and processed in research pathology lab. CD44 protein is a receptor for the ligand hyaluronic acid which causes inflammation in the heart valves. A total of 107 valves were studied. Of the 61 mitral valves studied 38 (62.2%) showed CD44 positivity as brownish granules within the cytoplasm. Of the 46 aortic valves studied 19 (41.3%) showed CD44 positivity as brownish granules within the cytoplasm. The valves showed evidence of past inflammation showing thick and thin walled blood vessels and lymphocytes. CD44 protein increased in 62.2% of mitral valves, of which 60.5% were from patients presenting when they were less than 40 years old. CD44 protein increased in 41.3% of aortic valves, of which 63.15% were from patients presenting when they were less than 40 years old. CD44 positivity was seen in 57 valves as brownish granules within the cytoplasm of the cell. CD44 protein increased in 53.27% of mitral and aortic valves, of which 63.15% were from the males patients. CD44 protein increased in 53.27% of mitral and aortic valves, of which 36.84% were from the females patients. Conclusion/Recommendations: The results showed CD44 is over expressed in the heart valves removed surgically for stenosis or regurgitation where the hyaluronic acid content is high. © 2010 Science Publications.


Ashokkumar M.,International Center for Cardio Thoracic and Vascular Diseases | Emmanuel C.,Global Hospitals and Health City | Dhandapany P.S.,Madurai Kamaraj University | Dhandapany P.S.,Mount Sinai School of Medicine | And 4 more authors.
DNA and Cell Biology | Year: 2011

The chromosomal region 9p21 has been reported to be associated with myocardial infarction, coronary artery disease (CAD), diabetes, and many other related multifactorial diseases in humans. Although the genome-wide association studies have identified a limited number of single-nucleotide polymorphisms (SNPs) at 9p21 for CAD risk, the role of flanking SNPs has not been studied so far. Therefore, in the present work, we studied the role of flanking SNPs with respect to that of the previously identified SNPs rs10757278 and rs2383207 at 9p21 among the Indian subjects found to have CAD (n=414) along with age- and sex-matched control subjects (n=408). Our study replicated the association of genome-wide association studies that had identified SNPs rs2383207 (p=4.7×10-5) and rs10757278 (p=5.5×10-5) among Indians with CAD. Further, we evaluated nine additional SNPs, of which two SNPs flanking rs2383207 (rs1537375 [p=2.4×10-5] and rs1537374 [p=5.6×10-5]) were also strongly associated with CAD. The haplotypes constructed using four risk SNPs revealed that the haplotypes with combinations of rs10757278 showed CAD risks, whereas the minor alleles of rs2383207, rs1537375, and rs1537374 in combinations reduce the CAD risks substantially. Our study demonstrates that the variation in the chromosomal region 9p21 is involved in modifying progression toward CAD among Indians and the risk may be variable, contributed by the SNPs that are flanking previously identified SNPs. © Copyright 2011, Mary Ann Liebert, Inc.


Rao Dr. G.S.N.,Tagore Medical College and Hospital | Gurumurthy P.,International Center for Cardio Thoracic and Vascular Diseases | Sruti E.,Tagore Medical College and Hospital
International Journal of Pharma and Bio Sciences | Year: 2013

The incidence of obesity among childhood in India is largely increasing. The serum apolipoproteins and its correlation with anthropometric and biochemical parameters were evaluated in 296 school children and adolescents (96 obese, 97 overweight, were compared with 103 normal controls, aged between 10-17 years). Anthropometric variables, lipid profile, fasting serum glucose analyzed by auto- analyzer and serum apolipoproteins by Immunoturbidimetry assay. Serum Apo-AI levels were decreased in obese and overweight children (130.94±9.24mg/dl, 132.26±10.80mg/dl Vs 153.08±17.98mg/dl) (p<0.001) than control children. Serum Apo-B levels were significantly elevated in obese and overweight children (88.54±11.4mg/dl,82.6±12.64mg/dl Vs 73.55±12.03mg/dl) (p<0.001) than controls. Serum Apo-B levels showed positive correlation with BMI, WHR, Apo-B/Apo-AI ratio, TCHR, TC, TG, LDL-C and showed negative correlation with fasting glucose levels, and no association with HDL-C. Serum Apo-AI levels showed negative correlation with BMI, WHR, Apo-B/Apo- AI ratio, TCHR, TC, TG, LDL-C and showed positive correlation with HDL-C and fasting glucose levels. These findings suggest that childhood obesity is associated with abnormal lipid patterns. Prevention campaigns aimed at identifying obese children and reducing their weight would probably also improve the lipid profile.


Srinivasa Nageswara Rao G.,Tagore Medical College and Hospital | Gurumurthy P.,International Center for Cardio Thoracic and Vascular Diseases | Sruti E.,Tagore Medical College and Hospital | Cherian K.M.,International Center for Cardio Thoracic and Vascular Diseases
Journal of Clinical and Diagnostic Research | Year: 2013

Results: The serum neopterin levels Introduction: An adverse pattern of blood lipids and cardiovascular abnormalities starts in obese children during childhood and neopterin serves as a marker for cardiovascular disease. Unfortunately, the data for children and adolescents, particularly, in the Indian population, are scarce. The present study aimed at evaluating the levels of serum neopterin in obese and overweight children and adolescents of the Indian population. Methods: The study groups included 296 school going children and adolescents. (96 obese and 97 overweight subjects were compared with 103 normal controls who were aged between 10- 17 years). The anthropometric variables, the lipid profile, the fasting serum glucose which was analyzed by using an autoanalyzer and the serum neopterin levels were assayed by HPLC (Shimazdu) by using the method of Palfrey et al., 1993. The serum insulin levels were measured by using ELISA kits. significantly in the obese (7.4±1.4) and overweight (6.4±0.8) (p<0.001) children and adolescents than in the controls (4.9±0.9). The serum neopterin levels showed a positive correlation with the BMI (r=0.79), WHR (r=0.5), systolic (r=0.44) and diastolic blood pressures (r= 0.25), insulin (r=0.57), HOMAIR (r=0.55), total cholesterol (r=0.35), triglycerides (r=0.20) and LDL-C (r=0.27) and they showed a negative correlation with HDL-C (r=-0.15) and fasting glucose (r= -0.3). Conclusion: This study revealed a good relationship between serum neopterin and the anthropometric and biochemical parameters. We, therefore, aim to conduct regular camps at schools to counsel and advise the identified overweight and obese children to go for physical exercise and a balanced diet. The implementation of preventive measures from early childhood will have far reaching benefits, as even the prevalence of other obesity related disorders could decline.


Kobayashi K.,Tokyo Institute of Technology | Pillai K.S.,International Center for Cardio Thoracic and Vascular Diseases | Guhatakurta S.,International Center for Cardio Thoracic and Vascular Diseases | Cherian K.M.,International Center for Cardio Thoracic and Vascular Diseases | Ohnishi M.,Tokyo Institute of Technology
Journal of Environmental Biology | Year: 2011

In the present study, an attempt was made to compare the statistical tools used for analysing the data of repeated dose toxicity studies with rodents conducted in 45 countries, with that of Japan. The study revealed that there was no congruence among the countries in the use of statistical tools for analysing the data obtained from the above studies. For example, to analyse the data obtained from repeated dose toxicity studies with rodents, Scheffé's multiple range and Dunnett type (joint type Dunnett) tests are commonly used in Japan, but in other countries use of these statistical tools is not so common. However, statistical techniques used for testing the above data for homogeneity of variance and inter-group comparisons do not differ much between Japan and other countries. In Japan, the data are generally not tested for normality and the same is true with the most of the countries investigated. In the present investigation, out of 127 studies examined, data of only 6 studies were analysed for both homogeneity of variance and normal distribution. For examining homogeneity of variance, we propose Levene's test, since the commonly used Bartlett's test may show heterogeneity in variance in all the groups, if a slight heterogeneity in variance is seen any one of the groups. We suggest the data may be examined for both homogeneity of variance and normal distribution. For the data of the groups that do not show heterogeneity of variance, to find the significant difference among the groups, we recommend Dunnett's test, and for those show heterogeneity of variance, we recommend Steel's test. © 2011 Triveni Enterprises.


Subash P.,International Center for Cardio Thoracic and Vascular Diseases | Premagurumurthy K.,International Center for Cardio Thoracic and Vascular Diseases | Sarasabharathi A.,International Center for Cardio Thoracic and Vascular Diseases | Cherian K.M.,International Center for Cardio Thoracic and Vascular Diseases
Journal of Human Hypertension | Year: 2010

To investigate the total antioxidant status (TAS) and the extent of oxidative DNA damage in total lymphocytes and their relation with essential hypertension. A total of 130 South Indian subjects aged 30-65 were recruited for the study. Of these hypertensive subjects investigated, 30 were newly diagnosed and were not on any antihypertensive drugs, but had systolic blood pressure (BP) ranging between 140 and 160 mm Hg and diastolic BP between 95 and 100 mm Hg; 50 hypertensive patients who were already on drug therapy for 1 year and 50 were normotensive controls with BP ≤120/80 mm Hg. DNA damage was significantly increased in hypertensive patients (both newly diagnosed and who were already on drug therapy) compared with control group. The major increase in DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who were already on drug therapy. There was a significant decrease in plasma TAS value in essential hypertensive groups as compared to normotensive controls. Lymphocyte DNA damage was independently correlated with only TAS. Lymphocyte DNA damage was increased in hypertensive patients. The major increase in lymphocyte DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who already on drug therapy. Decreased TAS levels, which reflect to increased oxidative stress, may be the reason of increased total lymphocyte DNA damage in South Indian hypertensive patients. © 2010 Macmillan Publishers Limited All rights reserved.


Rao G.S.N.,International Center for Cardio Thoracic and Vascular Diseases | Gurumurthy P.,International Center for Cardio Thoracic and Vascular Diseases | Gururajan P.,International Center for Cardio Thoracic and Vascular Diseases | Saibabu R.,International Center for Cardio Thoracic and Vascular Diseases | Cherian K.M.,International Center for Cardio Thoracic and Vascular Diseases
Pediatrics International | Year: 2011

Background: An adverse pattern of blood lipids and atherosclerosis begin in childhood. Unfortunately, data for children and adolescents, particularly those in the Indian population, are scarce. The present study aims to evaluate the levels of serum E-selectin in Indian children and adolescents and its correlation with anthropometric and biochemical parameters. Methods: The study groups included 338 school children and adolescents. There were 96 obese children, 97 overweight children and 42 children with congenital heart disease who were compared with 103 normal controls, aged 10-17 years. Serum E-selectin and serum leptin were analyzed with enzyme-linked immunosorbent assay kits. Lipid profile and fasting glucose were analyzed using an autoanalyzer. Results: Serum E-selectin levels were significantly increased in obese (65.3 ± 8.39 ng/mL) and overweight (56.01 ± 6.96 ng/mL) subjects (P < 0.001). However, these levels were lower in children with congenital heart disease (40.99 ± 6.54 ng/mL) than in controls (43.79 ± 6.71 ng/mL). Conclusion: Serum E-selectin levels showed good positive association with body mass index, waist-to-hip ratio, systolic and diastolic blood pressure, leptin, total cholesterol, triglycerides and low-density lipoprotein cholesterol and showed negative correlation with fasting glucose and no significant association with high-density lipoprotein cholesterol. These findings indicate that higher E-selectin levels can induce endothelial activation and play an essential role in the earliest stage of the atherosclerotic process in obese and overweight children. Regular camps at schools to counsel the identified overweight and obese children and to encourage physical exercise would help to reduce the risk of these children being prone to major cardiovascular anomalies in adulthood. © 2011 Japan Pediatric Society.


Srinivasa Nageswara Rao G.,International Center for Cardio Thoracic and Vascular Diseases | Prema G.,International Center for Cardio Thoracic and Vascular Diseases | Priya G.,International Center for Cardio Thoracic and Vascular Diseases | Arumugam S.B.,International Center for Cardio Thoracic and Vascular Diseases | And 3 more authors.
Indian Journal of Clinical Biochemistry | Year: 2011

There is a rising trend in the prevalence of insulin resistance among obese, overweight children and adolescents. The serum insulin and its correlation with biochemical, clinical and anthropometric parameters were evaluated in 185 children and adolescents (59 control, 52 obese, 49 overweight, 25 congenital heart disease) of age group 10-17 years. The levels of serum insulin were measured by ELISA. Serum insulin levels were found to be significantly increased in children who were obese, overweight and had congenital heart disease, than controls. Serum insulin levels positively correlated with BMI, WHR, and serum C-peptide, serum leptin, total cholesterol, triglycerides, LDL-cholesterol, systolic and diastolic blood pressure. Fasting glucose levels were found to be negatively correlated with serum insulin levels. HDL-cholesterol levels were non-significant among the study groups. We identified nine obese children (five girls and four boys) with the features of metabolic syndrome and 69% of obese and overweight children were identified with insulin resistance. Insulin resistance was strongly associated with metabolic syndrome and its components, especially with central obesity and hypertriglyceridemia. © 2010 Association of Clinical Biochemists of India.


PubMed | International Center for Cardio Thoracic and Vascular Diseases
Type: Journal Article | Journal: Cytotechnology | Year: 2012

Mesenchymal stem cells (MSCs) are multipotent, can be easily expanded in culture and hence are an attractive therapeutic tool for cardiac repair. MSCs have tremendous potential to transdifferentiate to cardiac lineage both in vitro and in vivo. The present study examined the differentiation capacity of conditioned media derived from ischemic cardiac tissue on human MSCs. Human Bone marrow-derived MSCs after due characterization by immunocytochemistry and flow cytometry for MSC specific markers were induced by culture media derived from ischemic (n=13) and non-ischemic (n=18) human cardiac tissue. Parallel cultures were treated with 5-azacytidine (5-azaC), a potent cardiomyogen. MSCs induced with ischemic conditioned media formed myotube like structures, expressed sarcomeric Troponin I, alpha myosin heavy chain proteins and were positive for cardiac specific markers (Nkx2.5, human atrial natriuretic peptide, myosin light chain-2a, GATA-4) as was observed in 5-azaC treated cells. However, uninduced MSCs as well as those induced with non-ischemic cardiac conditioned media still maintained the fibroblast morphology even after 3weeks post-induction. Transmission electron microscopic studies of cardiomyocyte-like cells derived from MSCs revealed presence of sarcomeric bands but failed to show gap junctions and intercalated discs as of adult cardiomyocytes. These findings demonstrate that ischemic cardiac conditioned media induces morphological and molecular changes in MSCs with cardiac features, but at a primitive stage. Proteomics analysis of the ischemic conditioned media revealed differential expression of three relevant proteins (C-type lectin superfamily member 13, Testis-specific chromodomain protein Y2 and ADP/ATP translocase 1), whose exact role in cardiac regeneration needs further analysis.

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