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Delgado T.C.,Intermediary Metabolism Group and Inorganic Biochemistry and Molecular Imaging Group | Delgado T.C.,Institute Investigaciones Biomedicas Of Madrid Alberto Sols Csic Uam | Barosa C.,Intermediary Metabolism Group and Inorganic Biochemistry and Molecular Imaging Group | Nunes P.M.,Intermediary Metabolism Group and Inorganic Biochemistry and Molecular Imaging Group | And 3 more authors.
PLoS ONE | Year: 2012

Sources of plasma glucose excursions (PGE) following a glucose tolerance test enriched with [U- 13C]glucose and deuterated water were directly resolved by 13C and 2H Nuclear Magnetic Resonance spectroscopy analysis of plasma glucose and water enrichments in rat. Plasma water 2H-enrichment attained isotopic steady-state within 2-4 minutes following the load. The fraction of PGE derived from endogenous sources was determined from the ratio of plasma glucose position 2 and plasma water 2H-enrichments. The fractional gluconeogenic contributions to PGE were obtained from plasma glucose positions 2 and 5 2H-positional enrichment ratios and load contributions were estimated from plasma [U- 13C]glucose enrichments. At 15 minutes, the load contributed 26±5% of PGE while 14±2% originated from gluconeogenesis in healthy control rats. Between 15 and 120 minutes, the load contribution fell whereas the gluconeogenic contribution remained constant. High-fat fed animals had significant higher 120-minute blood glucose (173±6 mg/dL vs. 139±10 mg/dL, p<0.05) and gluconeogenic contributions to PGE (59±5 mg/dL vs. 38±3 mg/dL, p<0.01) relative to standard chow-fed controls. In summary, the endogenous and load components of PGE can be resolved during a glucose tolerance test and these measurements revealed that plasma glucose synthesis via gluconeogenesis remained active during the period immediately following a glucose load. In rats that were placed on high-fat diet, the development of glucose intolerance was associated with a significantly higher gluconeogenic contribution to plasma glucose levels after the load. © 2012 Delgado et al.

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