Interactive Research School for Health Affairs

Pune, India

Interactive Research School for Health Affairs

Pune, India
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Mary S.,CSIR - National Chemical Laboratory | Kulkarni M.J.,CSIR - National Chemical Laboratory | Malakar D.,Sciex | Joshi S.R.,Interactive Research School for Health Affairs | And 2 more authors.
Journal of Proteome Research | Year: 2017

Pre-eclampsia is a hypertensive disorder characterized by the new onset of hypertension >140/90 mmHg and proteinuria after the 20th week of gestation. The disorder is multifactorial and originates with abnormal placentation. Comparison of the placental proteome of normotensive (n = 25) and pre-eclamptic (n = 25) patients by gel-free proteomic techniques identified a total of 2145 proteins in the placenta of which 180 were differentially expressed (>1.3 fold, p < 0.05). Gene ontology enrichment analysis of biological process suggested that the differentially expressed proteins belonged to various physiological processes such as angiogenesis, apoptosis, oxidative stress, hypoxia, and placental development, which are implicated in the pathophysiology of pre-eclampsia. Some of the differentially expressed proteins were monitored in the plasma by multiple reaction monitoring analysis, which showed an increase in apolipoproteins A-I and A-II in gestational weeks 26-30 (2-fold, p < 0.01), while haptoglobin and hemopexin decreased in gestational weeks 26-30 and week 40/at delivery (1.8 fold, p < 0.01) in pre-eclamptic patients. This study provides a proteomic insight into the pathophysiology of pre-eclampsia. Identified candidate proteins can be evaluated further for the development of potential biomarkers associated with pre-eclampsia pathogenesis. © 2016 American Chemical Society.

Pillai A.,Georgia Regents University | Pillai A.,Charlie Norwood Medical Center | Kale A.,Interactive Research School for Health Affairs | Joshi S.,Interactive Research School for Health Affairs | And 5 more authors.
International Journal of Neuropsychopharmacology | Year: 2010

Brain-derived neurotrophic factor (BDNF), which plays an important role in neurodevelopmental plasticity and cognitive performance, has been implicated in neuropsychopathology of schizophrenia. We examined the levels of both cerebrospinal fluid (CSF) and plasma BDNF concomitantly in drug-naive first-episode psychotic (FEP) subjects with ELISA to determine if these levels were different from control values and if any correlation exists between CSF and plasma BDNF levels. A significant reduction in BDNF protein levels was observed in both plasma and CSF of FEP subjects compared to controls. BDNF levels showed significant negative correlation with the scores of baseline PANSS positive symptom subscales. In addition, there was a significant positive correlation between plasma and CSF BDNF levels in FEP subjects. The parallel changes in BDNF levels in plasma and CSF indicate that plasma BDNF levels reflect the brain changes in BDNF levels in schizophrenia. Copyright © CINP 2009.

Malonia S.K.,National Center for Cell Science | Sinha S.,National Center for Cell Science | Lakshminarasimhan P.,Gothenburg University | Singh K.,Cleveland Clinic Lerner Research Institute | And 4 more authors.
Biochimica et Biophysica Acta - Reviews on Cancer | Year: 2011

Changes in the composition of nuclear matrix associated proteins contribute to alterations in nuclear structure, one of the major phenotypes of malignant cancer cells. The malignancy-induced changes in this structure lead to alterations in chromatin folding, the fidelity of genome replication and gene expression programs. The nuclear matrix forms a scaffold upon which the chromatin is organized into periodic loop domains called matrix attachment regions (MAR) by binding to various MAR binding proteins (MARBPs). Aberrant expression of MARBPs modulates the chromatin organization and disrupt transcriptional network that leads to oncogenesis. Dysregulation of nuclear matrix associated MARBPs has been reported in different types of cancers. Some of these proteins have tumor specific expression and are therefore considered as promising diagnostic or prognostic markers in few cancers. SMAR1 (scaffold/matrix attachment region binding protein 1), is one such nuclear matrix associated protein whose expression is drastically reduced in higher grades of breast cancer. SMAR1 gene is located on human chromosome 16q24.3 locus, the loss of heterozygosity (LOH) of which has been reported in several types of cancers. This review elaborates on the multiple roles of nuclear matrix associated protein SMAR1 in regulating various cellular target genes involved in cell growth, apoptosis and tumorigenesis. © 2010 Elsevier B.V.

Hussain A.J.,Directorate of Rice Research | Hussain A.J.,Seed Works India Pvt Ltd | Ali J.,International Rice Research Institute | Siddiq E.A.,Directorate of Rice Research | And 6 more authors.
Plant Breeding | Year: 2012

Genetic analysis of F 2 and backcross populations of an induced temperature-sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymorphic randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers through bulked segregant analysis. The RAPD primers UBC 345 830, UBC 313 927, microsatellites RM224 and RM21 produced putative markers, which differentiate parents and bulks from sterile parent and sterile bulks. The RAPD analysis of individual F 2 plants with the primer UBC345 830 showed perfect marker-phenotype cosegregation. The 830-bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33cM, was eluted and cloned, and sequence information was used for designing sequence-characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8cM. TGMS locus was mapped onto chromosome 11 using RM21 and RM224, flanking it at a distance of 4.3 and 3.0cM, respectively. The DNA markers tightly linked to TGMS gene (tms8) in F 61 can be cost effectively used for marker-assisted selection of TGMS trait. © 2011 Blackwell Verlag GmbH.

Bhalerao S.,Interactive Research School for Health Affairs | Hegde M.,Bharati Vidyapeeth Deemed University | Katyare S.,Bharati Vidyapeeth Deemed University | Kadam S.,Interactive Research School for Health Affairs
World's Poultry Science Journal | Year: 2014

Similar to omega-3 eggs, chicken meat has great potential to become a functional food for humans. In India, chicken meat is preferred due to its perceived health benefits and affordability. The balance between omega 3 and 6 fatty acids is crucial for its usefulness in animals. At present commercial chicken meat has low omega-3 fatty acid content and higher omega-6 fatty acid content. Published research shows that it is possible to modify the lipid profile of commercial chickens by manipulating the broiler diet. The modern human diet is deficient in n-3 FAs, which has been linked to the increase in several degenerative diseases such as cardiovascular disease, diabetes, arthritis, cancer and mental illness. Therefore omega-3 chicken meat may be an effective means of increasing n-3 FA in the human diet. There are several problems associated with the commercial production of omega-3 chicken meat related to the choice of source of fatty acids in the feed, cost of production, consumer acceptability and stability of the chicken meat that need to be tackled. The present article briefly reviews the studies carried out in this respect, the possible impact of omega-3 chicken meat production on the poultry industry, and on human health. © 2014 World's Poultry Science Association.

Roy S.,Interactive Research School for Health Affairs | Dhobale M.,Interactive Research School for Health Affairs | Dangat K.,Interactive Research School for Health Affairs | Mehendale S.,Bharati Vidyapeeth Deemed University | And 3 more authors.
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2014

Maternal long chain polyunsaturated fatty acids (LCPUFA) play a key role in fetal growth and development. This study for the first time examines the maternal and cord LCPUFA levels in preeclamptic mothers delivering male and female infants. In this study 122 normotensive control pregnant women (gestation≥37 weeks) and 90 women with preeclampsia were recruited. Results indicate lower maternal plasma docosahexaenoic acid (DHA) levels (p<0.05) in women with preeclampsia delivering male babies as compared to normotensive control women delivering male babies. Similarly, cord nervonic acid levels were lower (p<0.01) in women with preeclampsia delivering male babies as compared to normotensive control group. However, cord nervonic acid levels were comparable in women with preeclampsia and normotensive control women delivering female babies. This data suggests that male babies born to mothers with preeclampsia may be at an increased risk of developing neurodevelopmental disorders as compared to female babies. Future studies need to follow up both male and female children born to mothers with preeclampsia since altered levels of LCPUFA at birth may have differential implications for the growth and development. © 2014 Elsevier Ltd. All rights reserved.

Wani K.D.,Interactive Research School for Health Affairs | Kitture R.,Fergusson College | Ahmed A.,Interactive Research School for Health Affairs | Choudhari A.S.,Interactive Research School for Health Affairs | And 4 more authors.
Journal of Bionanoscience | Year: 2011

Curcumin, a naturally occurring polyphenol extracted from turmeric (Curcuma longa), an indispensable culinary spice in Indian diet, has been known to function as a potent anticancer agent. However, its limited solubility and absorption into body tissues limits its bioavailability. Magnetic nanoparticles, typically Fe 3O 4, have been shown to possess various biomedical applications including hyperthermia, MRI, magnetic separation and targeted drug delivery. In the present paper, we report the synthesis of a highly aqueous stable suspension of citric acid (CA) capped iron oxide (Fe 3O 4) nanoparticles conjugated to curcumin (CU). The significance of this report lies in the use of citric acid as a capping agent for magnetic nanoparticles in combination with curcumin. Citric acid has been mainly used to overcome the problem of limited bioavailability of curcumin as well as to impart ferrofluid property to the nanoparticles. The Curcumin-Citric acid-Fe 3O 4 nanoparticles (CCF) were duly characterized by X-ray diffraction (XRD) and conjugated nanoparticles were confirmed by Fourier transform infrared spectroscopy (FTIR). The percent drug loading was evaluated by thermo gravimetric analysis (TGA). CCF nanoparticles were studied in vitro to determine whether the curcumin retained its anticancer activity after conjugation with citric acid capped nanoparticles. In vitro uptake of CCF nanoparticles into breast cancer cell line, MCF7, was confirmed by Prussian blue staining. MTT assay was performed to determine cytotoxicity of the nanoparticles towards MCF7. Growth assays proved that the anticancer activity of curcumin was retained even after conjugation with CA. © 2011 American Scientific Publishers All rights reserved.

PubMed | Bharati Vidyapeeth Deemed University, Bharati Medical College and Hospital and and Interactive Research School for Health Affairs
Type: Journal Article | Journal: The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians | Year: 2015

Increased oxidative stress is known to be associated with pregnancy complications like preeclampsia (PE). We hypothesize that increased maternal oxidative stress may differentially affect/program the pregnancy outcome during early postnatal periods in male and female babies.One-hundred three healthy pregnant women (gestation 37 weeks) were recruited for the normotensive control (NC) group and 57 women with term-preeclampsia (T-PE; gestation 37 weeks) and 28 women with preterm-preeclampsia (PT-PE; gestation <37 weeks) were also recruited. All infants were followed for anthropometric measurements until six months of age.Higher maternal plasma malondialdehyde (MDA) and erythrocyte superoxide dismutase and glutathione peroxidase (GPx) levels were observed in both T-PE and PT-PE groups. Higher maternal levels of MDA and GPx were seen in mothers delivering male babies in T-PE and PT-PE groups, respectively, as compared to mothers delivering female babies. Babies born to mothers with PT-PE showed poor growth and development on all the anthropometric parameters compared to those born to mothers with T-PE and NC.The altered levels of oxidative stress and antioxidant enzymes in mothers with PE delivering male babies suggest that they may be at higher risk for developing metabolic and neurodevelopmental disorders than female babies.

PubMed | Bharati Vidyapeeth Deemed University and Interactive Research School for Health Affairs
Type: | Journal: Reproductive sciences (Thousand Oaks, Calif.) | Year: 2016

Children born preterm are reported to be at increased risk of developing noncommunicable diseases in later life. Altered placental DNA methylation patterns are implicated in fetal programming of adult diseases. Our earlier animal studies focus on micronutrients (folic acid, vitamin B

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