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Apeldoorn, Netherlands

Guo J.,Gongli Hospital of Shanghai Pudong New District | Guo W.,Intensive Care Unit ICU | Jin X.,Wannan Medical College | Liu Y.,Gongli Hospital of Shanghai Pudong New District | And 2 more authors.
International Journal of Clinical and Experimental Medicine

Aims: To investigate the effects of angiotensin II type 1 receptor (AT1R) antagonist, losartan, on rats with septic shock induced by endotoxin. Methods: Thirty male SD rats were randomly divided into 3 groups (10 for each group): rats were injected with normal saline in C group, lipopolysaccharide (LPS) of 12 mg kg-1 intravenously in LPS group, and losartan of 50 mg kg-1 intraperitoneally followed by LPS of 12 mg kg-1 intravenously in LOS group. The plasma concentrations of nitric oxide (NO), malondialdehyde (MDA), IL-1β and TNF-α were measured 6 h after LPS administration. Then all the rats were sacrificed immediately before the aortas pectoralis were isolated. Inhibitor of NF-κB (IκB) mRNA and its mRNA expressions in aorta were detected. Results: The plasma concentrations of NO, MDA, IL-1β and TNF-α were all significantly elevated in LPS group compared with the control group (P<0.01), which were markedly reduced in LOS group (P<0.01). Both of the mRNA and protein expressions of IκB in aorta were downregulated after injection of LPS when compared with the control group (P<0.01). However, IκB mRNA and protein expressions in aorta in the LOS group were significantly higher than the LPS group (P<0.01). Conclusion: AT1R antagonist, losartan, has a reverse effect at least partly on circulation dysfunction in rats with septic shock induced by endotoxin. © 2015, Int J Clin Exp Med. All rights reserved. Source

Cheng C.-W.,Hong Kong Baptist University | Kwok A.O.L.,Medical Center | Bian Z.-X.,Hong Kong Baptist University | Tse D.M.W.,Intensive Care Unit ICU
Evidence-based Complementary and Alternative Medicine

Constipation is a common problem in advanced cancer patients; however, specific clinical guidelines on traditional Chinese medicine (TCM) syndrome (Zhang) are not yet available. In this cross-sectional study, the TCM syndromes distribution and their common symptoms and signs among 225 constipated advanced cancer patients were determined. Results showed that 127 patients (56.4%) and 7 patients (3.1%) were in deficient and excessive patterns, respectively, while 91 patients (40.4%) were in deficiency-excess complex. The distributions of the five syndromes were: Qi deficiency (93.3%), Qi stagnation (40.0%), blood (Yin) deficiency (28.9%), Yang deficiency (22.2%), and excess heat (5.8%). Furthermore, age, functional status, and level of blood haemoglobin were factors related to the type of TCM syndrome. A TCM prescription with the functions on replenishing the Deficiency, redirecting the flow of Qi stagnation and moistening the dryness caused by the blood (Yin) deficiency can be made for the treatment of advance cancer patients with constipation. Robust trials are urgently needed for further justifying its efficacy and safety in evidence-based approaches. © 2012 Chung-Wah Cheng et al. Source

Fu T.-G.,Yishui Central Hospital | Wang L.,Linyi Peoples Hospital | Li W.,Peoples Care | Li J.-Z.,Intensive Care Unit ICU | Li J.,Linyi Peoples Hospital
International Journal of Molecular Medicine

Despite evidence that the crucial role of NUAK family, SNF1-like kinase, 2 [NUAK2; also known as sucrose non-fermenting 1 (SNF1)-like kinase (SNARK)], has been highlighted in cancer development and in tumor progression, to the best of our knowledge, there are no studies available to date on the role of NUAK2 in glioblastoma. Thus, in this study, in order to determine the role of NUAK2 in glioblastoma, we performed western blot analysis to detect its expression in glioma. The results demonstrated that NUAK2 expression was upregulated in glioma tissues and that its expression was associated with the advanced stages of the disease. In vitro, NUAK2 overexpression promoted the proliferation, migration and invasion of A172 glioblastoma cells, whereas the silencing of NUAK2 with a plasmid carrying shRNA targeting NUAK2 inhibited the proliferation of A172 glioblastoma cells. Moreover, NUAK2 regulated cancer stem cell (CSC)-related gene expression in the glioblastoma cells. We performed an analysis of potential microRNA (miR or miRNA) target sites using 3 commonly used prediction algorithms, miRanda, TargetScan and PicTar. All 3 algorithms predicted that miR143 targeted the 3'-untranslated region (3'UTR) of NUAK2. Subsequent experiments confirmed this prediction. Finally, we found that miR143 inhibited the proliferation, migration and invasion of the glioblastoma cells. Thus, the findings of the present study demonstrate that miR143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Source

Negahdary M.,Payame Noor University | Sadeghi S.A.,Islamic Azad University at Tonekabon | Hamrahi-Michak M.,Payame Noor University | Rezaei-Zarchi S.,Payame Noor University | And 4 more authors.
International Journal of Electrochemical Science

In this study, the direct electrochemistry of cytochrome c immobilized on a modified carbon paste electrode with cadmium oxide nanoparticles was described. The prepared cadmium oxide nanoparticles were characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The direct electron transfer of the immobilized cytochrome c exhibited two couples of redox peaks with the formal potentials of -0.305 V and -0.244 V (vs. SCE) in 0.1M (pH 7.0) PBS, respectively. The biosensor displayed an excellent electro-catalytical response to the reduction of H 2O 2. The linear range of this biosensor for H 2O 2 determination was from 20 to 210μM. The established biosensor exhibited fast response, high sensitivity, good reproducibility and stability. © 2012 by ESG. Source

Wang L.,Intensive Care Unit ICU | Wang L.,Guangzhou University | Zhang M.,Intensive Care Unit ICU | Zhang M.,Guangzhou University | And 12 more authors.
Evidence-based Complementary and Alternative Medicine

Objective. To determine the impact of an integrative medicine clinical pathways (CPs) on the length of in-hospital stay and on outcomes for patients with acute myocardial infarction (AMI). Methods. A multicenter nonrandomized controlled trial enrolling 197 consecutive patients with AMI at eight urban TCM hospitals was conducted between 1 January 2010 and 31 October 2010. These patients were enrolled in the interventional group after the CPs had been implemented. The control group included 405 patients with AMI from eight hospitals; these patients were treated between 1 January 2008 and 31 December 2009, before the CPs were implemented. Outcome measures were the length of hospital stay costs of medical care, and major cardiovascular events (MACEs) during hospitalization. Results. Compared with the control group, the patients in intervention group had a shorter length of hospital stay (9.2 ± 4.2 days versus 12.7 ± 8.6 days, P 0.05), and reduced healthcare costs in hospital (46365.7 ± 18266.9 versus 52866.0 ± 35404.4, P 0.05). There were statistically significant differences in MACE between the two groups during the hospitalization period (2.5 versus 6.9, P = 0.03). Conclusion. These data suggest that the development and implementation of the clinical pathways based in Integrative Medicine could further improve quality of care and outcome for patients with AMI. © 2012 Lei Wang et al. Source

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