Integria Healthcare

Ballina, Australia

Integria Healthcare

Ballina, Australia
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Adams D.H.,University of South Australia | Shou Q.,Southern Cross University of Australia | Wohlmuth H.,Southern Cross University of Australia | Wohlmuth H.,Integria Healthcare | Cowin A.J.,University of South Australia
Fitoterapia | Year: 2016

Australian native plants have a long history of therapeutic use in indigenous cultures, however, they have been poorly studied scientifically. We analysed the effects of 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for their potential wound healing properties by assessing their ability to induce or suppress Collagen I and Collagen III expression in human skin fibroblasts in culture. The compound 7-geranyloxycoumarin was able to significantly increase Collagen I (23.7%, p < 0.0002) expression in comparison to control. Significant suppression of Collagen III was observed for the compounds flindersine (11.1%, p < 0.02), and (N-acetoxymethyl) flindersine (27%, p < 0.00005). The implications of these finding is that these compounds could potentially alter the expression of different collagens in the skin allowing for the potential development of new wound healing therapies and new approaches for treating various skin diseases as well as photo (sun) damaged, and aged skin. © 2015 Elsevier B.V. All rights reserved.

Kasrati A.,Cadi Ayyad University | Alaoui Jamali C.,Cadi Ayyad University | Fadli M.,Cadi Ayyad University | Bekkouche K.,Cadi Ayyad University | And 5 more authors.
Industrial Crops and Products | Year: 2014

Thymus saturejoides is a perennial shrub widely distributed in the arid and semi arid parts of the Moroccan mountains. Essential oils (EOs) of this species are used extensively in food and pharmaceutical industries because of their wide biological and pharmacological properties. To our knowledge, no data are available on the antibacterial and antioxidant activities of T. saturejoides EOs in relation to the chemical variability of the species. Also, the potential synergistic interaction of EOs from this species as natural antimicrobial agent with conventional antibiotics against food-borne bacteria has not been investigated. Thus, the aim of the present study was to determine the volatile oil constituents of EOs from three wild Moroccan T. saturejoides populations collected from Northwest-Southeast of Morocco (June, 2011). Antioxidant activity and their antibacterial potency (singly and in combination with cefixime) against selected food-borne bacteria was then determined. Gas chromatography-mass spectrometry (GC-MS) analysis revealed 30 compounds, representing 98.6 to 99.5% of the total oils. The major constituents identified were carvacrol (45.3%), p-cymene (8.9%), linalool (8.4%), and borneol (7.5%) in oil from the Er-Rich population (arid site); carvacrol (26.5%), borneol (20.1%), camphene (8.0%), and γ-terpinene (5.6%) in oil from Ourika population (medium arid site) and carvacrol (25.3%), borneol (19.7%), camphene (7.6%), and p-cymene (6.6%) in oil from Taws population (less arid site). Essential oil obtained from Er-Rich population showed the highest antioxidant activity as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, reductive potential, and β-carotene/linoleic acid assays with IC50 values of 44.54±0.92μg/mL, 22.90±0.16μg/mL and 19.17±0.01μg/mL, respectively. Antibacterial tests showed that the oils from this species had a high inhibitory activity against tested bacteria, with the exception of Pseudomonas aeruginosa. The most potent activity was observed with oil obtained from the Er-Rich population with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.14 to 0.55mg/mL and 0.28 to 0.55mg/mL, respectively. Out of 21 combinations tested between EOs and cefixime, 67% showed total synergism, 19% had partial synergistic interaction and 14% showed no effect. Oil from the Er-Rich population exhibited the highest synergistic effect with antibiotic (FIC index values of 0.29 to 0.5). The synergy displayed by this combination of EO and cefixime may be of potential benefit for control of food-related bacteria, consequently permitting the use of lower doses of standard antibiotics. © 2014 Elsevier B.V.

El Abdouni Khiyari M.,Cadi Ayyad University | Kasrati A.,Cadi Ayyad University | Jamali C.A.,Cadi Ayyad University | Zeroual S.,Cadi Ayyad University | And 6 more authors.
Industrial Crops and Products | Year: 2014

Hydro-distilled essential oils (EOs) from the aerial parts of wild and cultivated Salvia aucheri subsp. blancoana, an endemic and threatened medicinal species of Morocco, were analyzed by GC/MS and screened for their antioxidant and insecticidal activities. In total 23 compounds representing more than 98.7% of the total oils were identified. Camphor (26.6-57.3%), camphene (6.3-22.0%), α-pinene (4.1-20.6%) and (E)-caryophyllene (5.4-5.7%) were found as the main constituents of the two oils. The EOs of the wild and cultivated material differed significantly in both the percentage of the main components and their biological activity. Camphor was more dominant in wild Moroccan sage oil (57.3%) than in cultivated sage (26.6%), while camphene (22.0%) and α-pinene (20.6%) were more abundant in the cultivated material compared to 6.3% and 4.1% respectively in wild sage. Regarding the biological activity, cultivated Moroccan sage oil showed the highest antioxidant activity, while the wild plant EO was the most effective against adults of the red flour beetle Tribolium castaneum Herbst. © 2014 Elsevier B.V.

Banbury L.K.,Southern Cross University of Australia | Shou Q.,Southern Cross University of Australia | Renshaw D.E.,Southern Cross University of Australia | Lambley E.H.,Southern Cross University of Australia | And 3 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Australian Aboriginal people used crushed leaves of Geijera parviflora Lindl. both internally and externally for pain relief, including for toothache (Cribb and Cribb, 1981). This study tested the hypothesis that this traditional use might be at least in part explained by the presence of compounds with anti-inflammatory activity. Materials and methods A crude extract (95% EtOH) was prepared from powdered dried leaves. From the CH3Cl fraction of this extract compounds were isolated by bioassay-guided fractionation and tested for: (1) cytotoxicity in RAW 264.7 murine leukemic monocyte-macrophages, (2) prostaglandin E2 (PGE2) inhibitory activity in 3T3 Swiss albino mouse embryonic fibroblast cells, as well as (3) nitric oxide (NO) and (4) tumour necrosis factor alpha (TNFα) inhibitory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Isolated compounds were also tested for (5) antibacterial activity against a panel of Gram-positive (Staphylococcus aureus ATCC 29213 and ATCC 25923, Staphylococcus epidermidis ATCC 35984, biofilm-forming) and Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) strains by broth microdilution. Results Eleven compounds were isolated, including one new flavone and one new natural product, with a further four compounds reported from this species for the first time. Some of the compounds showed good anti-inflammatory activity in vitro. In particular, flindersine (1) and N-(acetoxymethyl) flindersine (3) inhibited PGE2 release with IC50 values of 5.0 μM and 4.9 μM, respectively, without any significant cytotoxicity. Several other compounds showed moderate inhibition of NO (5, 6, 7) and TNF-α (6), with IC50 in the low micromolar range; however much of this apparent activity could be accounted for by the cytotoxicity of these compounds. None of the compounds showed anti-bacterial activity. Conclusions The inhibition of PGE2, an important mediator of inflammation and pain, by flindersine and a derivative thereof, along with the moderate anti-inflammatory activity shown by several other compounds isolated from Geijera parviflora leaf extract, support the traditional use of this plant for pain relief by Australian Aboriginal people. © 2015 Elsevier Ireland Ltd. All rights reserved.

Wohlmuth H.,Southern Cross University of Australia | Wohlmuth H.,Integria Healthcare | Savage K.,Southern Cross University of Australia | Dowell A.,Southern Cross University of Australia | Mouatt P.,Southern Cross University of Australia
Phytomedicine | Year: 2014

Extracts of ginkgo (Ginkgo biloba) leaf are widely available worldwide in herbal medicinal products, dietary supplements, botanicals and complementary medicines, and several pharmacopoeias contain monographs for ginkgo leaf, leaf extract and finished products. Being a high-value botanical commodity, ginkgo extracts may be the subject of economically motivated adulteration. We analysed eight ginkgo leaf retail products purchased in Australia and Denmark and found compelling evidence of adulteration with flavonol aglycones in three of these. The same three products also contained genistein, an isoflavone that does not occur in ginkgo leaf. Although the United States Pharmacopeia - National Formulary (USP-NF) and the British and European Pharmacopoeias stipulate a required range for flavonol glycosides in ginkgo extract, the prescribed assays quantify flavonol aglycones. This means that these pharmacopoeial methods are not capable of detecting adulteration of ginkgo extract with free flavonol aglycones. We propose a simple modification of the USP-NF method that addresses this problem: by assaying for flavonol aglycones pre and post hydrolysis the content of flavonol glycosides can be accurately estimated via a simple calculation. We also recommend a maximum limit be set for free flavonol aglycones in ginkgo extract. © 2014 Elsevier GmbH.

Kasrati A.,Cadi Ayyad University | Alaoui Jamali C.,Cadi Ayyad University | Bekkouche K.,Cadi Ayyad University | Wohlmuth H.,Southern Cross University of Australia | And 3 more authors.
Journal of Food Science and Technology | Year: 2015

This study describes the antioxidant and insecticidal activities of essential oils (EOs) of Mentha suaveolens subsp. timija, Thymus satureioides, Achillea ageratum, Cotula cinerea and Salvia officinalis widely used in Morocco as flavorings, food additives and preservatives. Sixty seven components were identified accounting for more than 95.0 % of the total oils. M. suaveolens subsp. timija oil had as main components menthone and pulegone. A. ageratum oil was particularly rich in artemisyl acetate and yomogi alcohol. The essential oil of T. satureioides was characterized by high contents of carvacrol and borneol. C. cinerea oil contained trans-thujone and cis-verbenyl acetate as major constituents, whereas S. officinalis oil was characterized by trans-thujone and camphor. Antioxidant activities were examined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), reducing power, β-carotene/linoleic acid bleaching and ABTS radical tests. In all assays, the highest antioxidant potency was observed in T. satureioides EO with IC50 values ranging from 0.15 ± 0.36 μg mL-1 to 0.23 ± 0.67 μg mL−1 across the four assays. The in vitro evaluation of the insecticidal activity showed that M. suaveolens subsp. timija EO present the highest insecticidal efficiency against adults of Tribolium castaneum with LD50 and LD90 values of 0.17 μL cm−2 and 0.26 μL cm−2, respectively and LT50, LT90 values ranged from 44.19 h to 2.98 h and 98.14 h to 6.02 h, respectively. Our data support the possible use of T. satureioides oil as potential antioxidant agent, while M. suaveolens subsp. timija oil can be developed as a new natural bio-insecticide. © 2014, Association of Food Scientists & Technologists (India).

Matovic N.J.,University of Queensland | Hayes P.Y.,University of Queensland | Penman K.,Integria Healthcare | Lehmann R.P.,Integria Healthcare | De Voss J.J.,University of Queensland
Journal of Organic Chemistry | Year: 2011

The synthesis of 20 alkyl amides, including 15 naturally occurring polyunsaturated alkyl amides previously identified from Echinacea spp. (1-13 and 62) or from Achilla sp. (55) and five previously unknown geometric isomers (23, 28, 67, 73, and 80), is described. Importantly, these amides include all of the major alkyl amides present in commercially used Echinacea extracts. The syntheses demonstrate methodology used for constructing alkyl amides containing conjugated diyne and isomerically pure enyne and diene moieties and may be adapted easily for the preparation of other alkyl amides present in Echinacea spp. Terminal-conjugated diynes were prepared by a Cadiot-Chodkiewitz coupling/deprotection sequence utilizing a protected bromoacetylene, and methyl-substituted diynes were made via a base-catalyzed rearrangement of terminal-skipped diynes. Conjugated dienes were prepared conveniently and with high stereoselectivity by the reduction of enynes or diynes with Rieke zinc. With the exception of 1-2 and 11-12, the alkyl amides are synthesized here for the first time, and their NMR data are consistent with that of the reported isolated natural compounds. © 2011 American Chemical Society.

Challinor V.L.,University of Queensland | Parsons P.G.,Queensland Institute of Medical Research | Chap S.,University of Queensland | White E.F.,University of Queensland | And 3 more authors.
Steroids | Year: 2012

Phytochemical characterization of a commercial herb sample supplied as Smilax ornata Lem. (sarsaparilla) led to the isolation of five steroidal saponins, including two new furostanol saponins sarsaparilloside B (1) and sarsaparilloside C (2), whose structures were elucidated via a combination of multistage mass spectrometry (MS n), 1D and 2D NMR experiments, and chemical degradation. The previously unreported spectroscopic characterization of sarsaparilloside (3), Δ 20(22)-sarsaparilloside (4), and parillin (5) is also provided. The antiproliferative activity of the isolated saponins was compared in six human cell lines derived from different tumor types and one of the structures (2) was particularly active against the HT29 colon tumor cell line. © 2011 Elsevier Inc. All rights reserved.

Challinor V.L.,University of Queensland | Stuthe J.M.U.,University of Queensland | Parsons P.G.,Queensland Institute of Medical Research | Lambert L.K.,University of Queensland | And 3 more authors.
Journal of Natural Products | Year: 2012

Figure Persented: Phytochemical investigation of Chamaelirium luteum ("false unicorn") resulted in the isolation of 15 steroidal glycosides. Twelve of these (1, 2, 4-9, 11-13, and 15) are apparently unique to this species, and eight of these (6-9, 11-13, and 15) are previously unreported compounds; one (15) possesses a new steroidal aglycone. In addition, the absolute configuration of (23R,24S)-chiograsterol A (10) was defined, and its full spectroscopic characterization is reported for the first time. The structures and configurations of the saponins were determined using a combination of multistage mass spectrometry (MSn), 1D and 2D NMR experiments, and chemical degradation. The antiproliferative activity of nine compounds obtained in the present work, and eight related compounds generated in previous work, was compared in six human tumor cell lines, with aglycones 3 and 10 and related derivatives 16, 17, 19, and 20 all displaying significant antiproliferative activity. © 2012 The American Chemical Society and American Society of Pharmacognosy.

Challinor V.L.,University of Queensland | Stuthe J.M.U.,University of Queensland | Bernhardt P.V.,University of Queensland | Lehmann R.P.,Integria Healthcare | And 2 more authors.
Journal of Natural Products | Year: 2011

Investigation of Chamaelirium luteum roots led to the isolation of two new steroidal saponins, helosides A (1) and B (2), that contain a previously unreported aglycone, helogenin (3). Their structures and absolute configuration were elucidated through MS-MS, NMR, chemical degradation, and X-ray crystallography. © 2011 The American Chemical Society and American Society of Pharmacognosy.

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