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Zhao J.,CAS Institute of Biophysics | Li Y.,Integrative Medicine Center | Jin L.,Institute of Translational Hepatology | Zhang S.,Institute of Translational Hepatology | And 8 more authors.
PLoS ONE | Year: 2012

Natural killer (NK) cells are abundant in the liver and have been implicated in inducing hepatocellular damage in patients with chronic hepatitis B virus (HBV) infection. However, the role of NK cells in acute HBV infection remains to be elucidated. We comprehensively characterized NK cells and investigated their roles in HBV clearance and liver pathology in 19 chronic hepatitis B (CHB) patients and 21 acute hepatitis B (AHB) patients as well as 16 healthy subjects. It was found that NKp46+ NK cells were enriched in the livers of AHB and CHB patients. We further found that peripheral NK cells from AHB patients expressed higher levels of activation receptors and lower levels of inhibitory receptors than those from CHB patients and HC subjects, thus displaying the increased cytolytic activity and interferon-γ production. NK cell activation levels were also correlated positively with serum alanine aminotransferase levels and negatively with plasma HBV DNA levels in AHB patients, which is further confirmed by the longitudinal follow-up of AHB patients. Serum pro-inflammatory cytokine and chemokine levels were also increased in AHB patients as compared with CHB and HC subjects. Thus, the concomitantly increased interferon-γ and cytotoxicity of NK cells were associated with liver injury and viral clearance in AHB patients. © 2012 Zhao et al. Source

Sun Z.,Chinese Institute of Materia Medica | Sun Z.,Chengdu University of Traditional Chinese Medicine | Zhao Y.,Chinese Institute of Materia Medica | Liu T.,Chinese Institute of Materia Medica | And 6 more authors.
Thermochimica Acta | Year: 2013

The fingerprints of Aconitum L. plants, Radix Aconiti (RA), Radix Aconiti Singularis (RAS), Radix Aconiti Kusnezoffii (RAK), Radix Aconiti Lateralis Preparata (RALP) and Radix Aconiti Brachypodi (RAB), were established by ultra-high performance liquid chromatography (UPLC) and the metabolism of Aconitum L. plants on Escherichia coli (E. coli) were studied by microcalorimetry. The spectrum-effect relationships between UPLC fingerprints and bacterial metabolism were investigated using canonical correlation analysis (CCA). Because of their inherent differences of chemical compositions, the main actives of bacterial metabolism were different among five Aconitum L. plants. The potency bioactivity sequence of the tested five Aconitum L. products on E. coli was obtained that were RAB > RALP > RAK > RAS > RA. This work establishes a universal model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Aconitum L. plants, which offers some references for detecting principle components of traditional Chinese medicine on bioactivity. © 2013 Elsevier B.V. Source

Stump J.L.,Integrative Medicine Center | Retzlaff-Roberts D.,University of South Alabama | Boltz P.,Boltz Pain Management
Journal of Alternative and Complementary Medicine | Year: 2013

Objectives: The objective of this case study was to use integrative medicine to treat a debilitating and long-standing case of blepharospasm. Subject: The patient suffered from blepharospasm, which conventional medicine had failed to alleviate despite numerous and varied attempts over the course of 3 years of treatment. Blepharospasm involves the uncontrolled or abnormal blinking or twitching of the eyelids, which impairs the ability to see. The patient's symptoms were so severe that she was unable to work, drive, or in general lead a normal life. Intervention: Conventional and Chinese medicine practices were used in examining and diagnosing the patient. Treatment involved 6 weeks of electroacupuncture. Results: The patient's symptoms were reduced from severe to nearly nonexistent, and this improvement persisted at least 6 months after treatment ended. Conclusions: While this study involved only 1 patient, it demonstrates the effectiveness of electroacupuncture as a treatment for blepharospasm in a case where the patient was experiencing debilitating symptoms, which conventional allopathic treatment had failed to alleviate. This suggests that electroacupuncture may be similarly effective in treating other cases of blepharospasm. © Copyright 2013, Mary Ann Liebert, Inc. 2013. Source

Zhao J.,Research Center for Biological Therapy | Zhang Z.,Research Center for Biological Therapy | Luan Y.,CAS Institute of Biophysics | Zou Z.,Center for Non Infectious Liver Diseases | And 8 more authors.
Hepatology | Year: 2014

It is well established that interleukin (IL)-22 has hepatoprotective and antifibrotic functions in acute liver injury models; however, its function in patients with liver fibrosis and liver cirrhosis (LC) remains obscure. In the current study, we demonstrated that expression of numerous IL-22 pathway-associated genes was significantly up-regulated in hepatitis B virus (HBV)-infected liver tissues, compared to normal controls, through microarray analysis. In agreement with these findings, liver-infiltrating IL-22+ cells were largely increased in HBV-infected patients with LC, compared to those without LC or healthy subjects, and were positively associated with liver fibrosis staging scores. Immunohistochemistry and flow cytometric analyses revealed that IL-22 was produced by multiple intrahepatic immune cells and, preferentially, by T-helper (Th) 17 cells in LC patients. In an HBV transgenic (Tg) mouse model of T-cell-mediated chronic liver inflammation and fibrosis, blockade of IL-22 attenuated hepatic expression of chemokine (C-X-C motif) ligand 10 and chemokine (C-C motif) ligand 20 (CCL20) and subsequently reduced Th17 recruitment and liver inflammation and fibrosis progression. In vitro treatment with IL-22 stimulated hepatic stellate cells (HSCs) to secrete several chemokines and subsequently promoted Th17 cell chemotaxis. Blocking C-X-C chemokine receptor type 3 or CCL20 reduced Th17 cell chemotaxis by IL-22-treated HSCs. Conclusions: IL-22 plays a pathological role in exacerbating chronic liver inflammation and fibrosis by recruiting hepatic Th17 cells in HBV-infected patients and HBV Tg mice. © 2014 by the American Association for the Study of Liver Diseases. Source

Niemtzow R.C.,Integrative Medicine Center | Belard J.-L.,NATO Group on Integrative Medicine | Nogier R.,Groupe Lyonnais dEtudes Medicales
Medical Acupuncture | Year: 2015

Background: Acupuncture originated in China more than 5000 years ago. Battlefield Acupuncture (BFA), developed by Niemtzow, consists of treating 5 points on each ear, using semi-permanent needles to reduce pain in a few minutes. Easily taught to North Atlantic Treaty Organization (NATO) troops, this methodology can be explained in 3 hours. Objective: This article describes how the BFA technique may be taught to military medical personnel in a few hours and may be used in a NATO medical treatment facility or battlefield environment without the necessity for patients' disrobing. Methods: Five tiny, sterile 2-mm needles are inserted into specific points of each ear. The points are: (1) Cingulate Gyrus; (2) Thalamus; (3) Omega 2; (4) Shen Men; and (5) Point Zero. The needles may remain in the ears for up to 3 days. Conclusions: BFA produces rapid pain relief in a few minutes with almost no side-effects. This is an ideal technique to use when pain has not responded to narcotics or when habit-forming drugs are not desired, especially during critical military missions. © Mary Ann Liebert, Inc. 2015. Source

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