Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka

Skierniewice, Poland

Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka

Skierniewice, Poland

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Podwyszynska M.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Gabryszewska E.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Korbin M.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Jasinski A.,Gospodarstwo Ogrodnicze
Biotechnologia | Year: 2010

The genetic fidelity of the micropropagated daylily plants was evaluated by a phenotypic observation and with the molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques. The plants of nine daylily cultivars propagated in vitro for the period of one to five years and grown outdoors in the years 2002 to 2007 were used in the study. The somaclonal variants occurred within the micropropagated plants of the five genotypes. The somaclones differed from the true-to-type plants in terms of colour and shape of the flowers. Depending on the genotype, the frequency of somaclonal variation (SV) ranged from 1.4% to 100%. The RAPD and ISSR analyses of the selected somaclones confirmed the polymorphism between true-to-type plants and somaclones on the level of DNA.


Orlikowska T.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Sobiczewski P.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Zawadzka M.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Zenkteler E.,Adam Mickiewicz University
Biotechnologia | Year: 2010

Bacterial contamination is a serious problem in plant tissue culture. In in vitro cultures, bacteria are introduced, with the initial which is most frequently explants, but bacterial contamination can also come from the laboratory environment or from the staff themselves. Exogenous bacteria are easier to deal with, but endogenous bacteria remain problematic. Standard sterilization with ethanol, NaOCl or HgCl2 and with antibiotics can now be enriched with new components (NaDCC, AgNO3, nano-silver) or sanitation products (PPMTM, ProClin® 300, Biosept 33 SL, Vitrofural®, Dekaben). Some of these can be incorporated into a multiplication and rooting medium for one or more passages, if they are not phytotoxic to the plant explants. A special problem is presented by cryptic or viable but not cultivable bacteria which can be unable to multiply during many passages, but finally be disclosed in mass. The issue is, therefore, to find and apply tools for detection of selective media and/or molecular markers. The above questions are discussed in the present paper based on the literature and results of our own study.


Podwyszynska M.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Kuras A.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Korbin M.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka
Biotechnologia | Year: 2010

The aim of the study was an early detection of somaclonal variation (SV) which could occur within the micropropagated plant material. Shoot cultures of the Polish cultivars were used. Five cultivars derived from breeder A and four from breeder B. They were propagated in vitro for one or two years. The molecular markers (inter-simple sequence repeat, ISSR) were utilized for analysis of putative DNA polymorphism between standard plants (propagated traditionally) belonging to tested cultivars and somaclonal variants derived from them. Within the nine studied genotypes, for five of them, the ISSR analysis performed with ten primers did not reveal polymorphism between standard and micropropagated plants. The analysis of the other four cultivars, all derived from breeder B, showed that some of the plants, micropropagated either for one or two years, differed slightly from standard. Basing on ISSR data, the UGMA dendrogram showing genetic similarity of the analysed plants was generated and clusters grouping cultivars, their standards and micropropagated plants were noted.


Gabryszewska E.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka | Kawa-Miszczak L.,Instytut Sadownictwa i Kwiaciarstwa im. Szczepana Pieniazka
Biotechnologia | Year: 2010

The influence of sucrose concentration (10 and 30 g l-1), low level of nitrogen salts (1/8 KNO3, 1/8 NH3NO4), auxins (IBA 1 mg l-1 + IAA 1 mg l-1 + NAA 0,01 mg l -1), and temperature (15°C, 20°C) on the rooting in vitro and acclimatization in the greenhouse of two cultivars ('Jadwiga', 'Professor Wójcicki') of Paeonia lactiflora were investigated. The level of endogenous carbohydrates in the peony shoots and roots during the rooting and acclimatization phases were analysed. There were a higher rooting and acclimatization percentage of 'Professor Wójcicki' cultivar than 'Jadwiga' cultivar. Also, more roots were produced by shoots of 'Professor Wójcicki' cultivar. The presence of auxins in the medium and the higher concentration of sucrose (30 g l-1) stimulated number of roots/shoot. On the other hand, the higher rooting percentage was found on the auxin-free medium in the presence of high level of sucrose. The shoots of 'Professor Wójcicki' rooted best when cultured at 20°C, but the shoots of 'Jadwiga' at lower temperature - 15°C. During the rooting stage, the major sugars detected in the peony microplants were sucrose, glucose and fructose. After four weeks of acclimatization, the plantlets accumulated starch and showed strong inhibition of shoot growth, and dormant buds were developed.


Patent
Instytut Sadownictwa I Kwiaciarstwa Im. Szczepana Pieniazka | Date: 2010-06-09

A fan assembly for a spraying machine, usable in orcharding spraying machines for effecting spraying in orchards. The fan assembly of the invention, on a wheeled chassis, in addition to a tank (11) with the associated assemblies, has two frames mounted spaced apart along the symmetry axis of a supporting frame (9): a flat broken frame (1) behind a fan (5) and a flat front frame (2) in front of the fan (5). The fan (5) is a radial fan and it has at the inlet a multi-segment diaphragm choke valve (5.3) and a solid collector outlet connection port (5.5) with a rotational plate choke valve (5.7), mounted to a shaft (5.8.3). Both choke valves (5.3) and (5.7) are powered by electric motors (5.4) and (5.8). The assembly is constructively capable to cooperate with an electronic control system that operates suitably to different requirements of diverse protection program schemes.

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