Instrumentation and Environmental Science

Mohanpur, India

Instrumentation and Environmental Science

Mohanpur, India

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Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
American Journal of Plant Physiology | Year: 2010

The genus Allium, consists of hundreds of medicinal plant species, is one of the most imperative sources of life supporting drugs. The in vitro biotechnological interventions are vital to choose, multiply, store up and improve the major Allium sp. In vitro culture of Allium has performed an incredibly crucial role in accelerated growth of several species with desirable traits and production of healthy and disinfectant propagules as well as paved the way towards cultivar improvement. During the last quite a few years, several moves have been made for in vitro propagation of Allium. In vitro regeneration via direct and indirect organogenesis using different explants and plant growth regulator formulations has been comprehensively covered in the literature. Recent challenges for establishment of protocols for genetic transformation have gained preference in the recent past reports. This review article comprehensively describes the exploitation of biotechnology for in vitro regeneration and genetic transformation for enhancement of the genus Allium. © 2010 Academic Journals Inc.


Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
In Vitro Cellular and Developmental Biology - Plant | Year: 2010

A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mg l-1) and BAP (1.5 mg l-1) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mg l-1) and 60 mg l-1 ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l-1 IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition. © 2010 The Society for In Vitro Biology.


Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
Biotechnology | Year: 2010

Aloe (Aloe vera L., Family Liliaceae), with proven multiple medicinal values finds favour readily in Ayurevdic applications, is facing a serious threat to its population as well as biodiversity due to its popularly harvested aloe leaves by the local communities and herbal medicine vendors. To succeed in dealing with the specified problem along with the facilitation of the mass production of commercial level, constant supply of quality propagules can be possible through conservation of propagules in vitro. The present study is thus concerned with in vitro conservation of multiple shoot culture of aloe to achieve unbroken supply of propagules maintaining their genetic purity. Rhizomatous stem explants result multiple bud break in MS plus 0.25 mg L -1 of NAA and 1.5 mg L-1 of BAP. Separated shoot buds further result in shoot multiplication and proliferation on MS with 2.5 mg L-1 of BAP. In vitro generated multiple shoots were split into individual shoot and subcultured for further multiplication in a sustainable. Five subcultures were performed at an extended 5 month interval over a period of 25 months in the same medium. Plantlets regenerated after 1 st subculture and plantlets from 5th subculture showed no significant difference in the phenotypic response. Genetic integrity of in vitro clones was tested using ISSR primers. All monomorphic bands in the ISSR assay, both for primary culture as well long term culture, ascertained their genetic integrity to a great extent. © 2010 Asian Network for Scientific Information.


Gantait S.,Instrumentation and Environmental Science | Mandai N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Kanti Das P.,Bidhan Chandra Krishi Viswavidyalaya
Biotechnology | Year: 2010

Regeneration efficiency and genetic clonality are two major aspect of successful long-term in vitro culture to be studied extensively. The regeneration efficiency of 25 months sustained in vitro culture of Allium ampeloprassum L. was maintained through multiple shoot proliferation and root growth. No morphological variation was detected between the plantlets regenerated from initial and long-term shoot tip culture or with their mother, meaning a high likelihood of their clonal fidelity. Detection of genetic integrity for in vitro regenerated clones was carried out using 10 ISSR primers among which 4 primers produced a total number of 336 distinct and scorable bands with an average of 7 bands per primers where the other 6 primers were not reproducible. The ISSR primers base on AG motif and 3 ' anchoring produced more number of consistent bands. All monomorphic bands in the ISSR assay both for primary culture as well long term culture in vitro ascertained to a great extent their genetic integrity through their partial genetic coverage. © 2010 Asian Network for Scientific Information.


Gantait S.,University Putra Malaysia | Das A.,Bihar Agricultural University | Mandal N.,Instrumentation and Environmental Science
Sugar Tech | Year: 2015

The present review illustrates the pharmacological properties and production of planting materials through in vitro organogenesis of Steviarebaudiana (Bertoni). The plant is native to Paraguay; however, the main producers of stevia are Japan, China, Taiwan, Thailand, Korea, Brazil, Malaysia and India. This plant is recorded as having a non-caloric natural sugar, alternative to artificially produced sugar substitutes and hence traditionally has been used to sweeten beverages. This article enumerates an overview on pharmacological and micropropagation aspects which are of use to researchers for further exploration for the indispensable improvement of this potential herb with medicinal importance. © 2014, Society for Sugar Research & Promotion.


Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
Natural Product Research | Year: 2011

An innovative protocol on accelerated in vitro propagation and acclimatisation was developed in Aloe vera L. Culture was initiated with rhizomatous stem where Murashige and Skoog (MS) medium fortified with 0.5 mg L -1 α-naphthalene acetic acid and 1.5 mg L -1 N 6-benzylaminopurine (BAP) promoted earliest shoot induction. Maximum shoot multiplication was achieved in MS medium supplemented with 2.5 mgL -1BAP. The best in vitro rooting was observed in the MS medium with 0.5 mg L -1 indole-3-acetic acid plus 2 gL -1 activated charcoal. The simple acclimatisation process, primarily with a combination of sand and soil (1 : 1 v/v) and finally with a blend of sand, soil and farm yard manure (2 : 1 : 1 v/v), ensured a 98% survival rate. Overall, 192 true-to-type plantlets were achieved from a single explant within 85 days. Morphologically, in vitro generated plants performed better than conventionally propagated plants; nevertheless the similarity in aloin content, gel content and superoxide dismutase activity was corroborated. © 2011 Taylor & Francis.


Telem R.S.,Bidhan Chandra Krishi Viswavidyalaya | Wani S.H.,SKUAST K | Singh N.B.,COA | Nandini R.,UAS | And 3 more authors.
Current Genomics | Year: 2013

The implication of molecular biology in crop improvement is now more than three decades old. Not surprisingly, technology has moved on, and there are a number of new techniques that may or may not come under the genetically modified (GM) banner and, therefore, GM regulations. In cisgenic technology, cisgenes from crossable plants are used and it is a single procedure of gene introduction whereby the problem of linkage drag of other genes is overcome. The gene used in cisgenic approach is similar compared with classical breeding and cisgenic plant should be treated equally as classically bred plant and differently from transgenic plants. Therefore, it offers a sturdy reference to treat cisgenic plants similarly as classically bred plants, by exemption of cisgenesis from the current GMO legislations. This review covers the implications of cisgenesis towards the sustainable development in the genetic improvement of crops and considers the prospects for the technology. © 2013 Bentham Science Publishers.


Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Nandy S.,Crop Quality Control
American Journal of Biochemistry and Molecular Biology | Year: 2011

Aromatic plants have been used commercially as spices, natural flavor, raw material for essential-oil industry and other medicinal purpose. Tropical and sub-tropical Asia are rich in the number of aromatic plant species due to their suitable ecological conditions. Micro propagation has superiority over conventional method of propagation because of high multiplication rate but, field performance of these tissue cultured plants depends on the selection of the initial material, media composition, growth regulators, cultivar and environmental factors. Some well developed in vitro techniques are currently available to help growers for meet the demand of the spices and pharmaceutical industry. Identification of somatic clones of plants derived through tissue culture can facilitate commercially viable in vitro propagation for medicinal and aromatic plants. An overview of the regeneration of aromatic plants by in vitro organogenesis from various types of explants is presented in this review article. © 2011 Academic Journals Inc.


Das A.,Instrumentation and Environmental Science | Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science
International Journal of Agricultural Research | Year: 2011

To sustain the supply of quality propagules, the present study was carried out to develop a novel protocol for accelerated in vitro mass multiplication in stevia (Stevia rebaudiana Bert.) through multiple shoot induction using shoot tips, nodal segments and axillary bud explants. The earliest bud induction was recorded from the shoot tip explants within 6 days of culture in Murashige and Skoog (MS) medium, in comparison to the other two explants. MS basal medium supplemented with sucrose (30 g L-1), agar (7 g L-1) and kinetin (2 mg L-1) performed best in multiple shoot proliferation, resulting more than 11 multiple shoots from a single shoot tip explant within 35 days of culture. For root induction and elongation, MS medium devoid of plant growth regulator performed most dynamically, where MS medium plus indole-3-acetic acid and 6-benzylaminopurine showed an adverse effect, promoting undesirable callus growth at root zone. In vitro generated propagules were successfully acclimatized in a balanced mixture of sand, soil and farm yard manure (1:1:1 v/v). Peroxidase assay along with ISSR fingerprinting confirmed the genetic clonality of in vitro generated propagules. © 2011 Academic Journals Inc.


Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
Plant Cell, Tissue and Organ Culture | Year: 2011

To induce variation through chromosome doubling in Gerbera jamesonii Bolus cv. Sciella, two-week-old in vitro grown shoots were treated with various concentrations of colchicine (0. 01, 0. 05, 0. 10, 0. 50 or 1% w/v) for 2, 4 or 8 h. Treated shoots were then cultured on Murashige and Skoog (MS) medium supplemented with 8. 8 μM 6-benzyladenine (BA) and 155 μM adenine sulphate (ADS), and subsequently transferred to fresh MS medium containing 2. 85 μM indole-3 acetic acid (IAA) for rooting. When shoots were treated with 0. 1% colchicine for 8 h, 64% of recovered plantlets were tetraploid. Ploidy of plantlets was confirmed by flow cytometry, stomatal analysis, and morphological characters. Tetraploid plantlets displayed slower proliferation along with higher vigor and thickened broad leaves. Moreover, tetraploid plants developed larger flowers, longer stalks, and have improved vase-life, all contributing to higher ornamental value of gerbera. © 2011 Springer Science+Business Media B.V.

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