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Telem R.S.,Bidhan Chandra Krishi Viswavidyalaya | Wani S.H.,SKUAST K | Singh N.B.,C.O.A. | Nandini R.,UAS | And 3 more authors.
Current Genomics | Year: 2013

The implication of molecular biology in crop improvement is now more than three decades old. Not surprisingly, technology has moved on, and there are a number of new techniques that may or may not come under the genetically modified (GM) banner and, therefore, GM regulations. In cisgenic technology, cisgenes from crossable plants are used and it is a single procedure of gene introduction whereby the problem of linkage drag of other genes is overcome. The gene used in cisgenic approach is similar compared with classical breeding and cisgenic plant should be treated equally as classically bred plant and differently from transgenic plants. Therefore, it offers a sturdy reference to treat cisgenic plants similarly as classically bred plants, by exemption of cisgenesis from the current GMO legislations. This review covers the implications of cisgenesis towards the sustainable development in the genetic improvement of crops and considers the prospects for the technology. © 2013 Bentham Science Publishers.

Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
In Vitro Cellular and Developmental Biology - Plant | Year: 2010

A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mg l-1) and BAP (1.5 mg l-1) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mg l-1) and 60 mg l-1 ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l-1 IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition. © 2010 The Society for In Vitro Biology.

Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
American Journal of Plant Physiology | Year: 2010

The genus Allium, consists of hundreds of medicinal plant species, is one of the most imperative sources of life supporting drugs. The in vitro biotechnological interventions are vital to choose, multiply, store up and improve the major Allium sp. In vitro culture of Allium has performed an incredibly crucial role in accelerated growth of several species with desirable traits and production of healthy and disinfectant propagules as well as paved the way towards cultivar improvement. During the last quite a few years, several moves have been made for in vitro propagation of Allium. In vitro regeneration via direct and indirect organogenesis using different explants and plant growth regulator formulations has been comprehensively covered in the literature. Recent challenges for establishment of protocols for genetic transformation have gained preference in the recent past reports. This review article comprehensively describes the exploitation of biotechnology for in vitro regeneration and genetic transformation for enhancement of the genus Allium. © 2010 Academic Journals Inc.

Gantait S.,Instrumentation and Environmental Science | Mandai N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Kanti Das P.,Bidhan Chandra Krishi Viswavidyalaya
Biotechnology | Year: 2010

Regeneration efficiency and genetic clonality are two major aspect of successful long-term in vitro culture to be studied extensively. The regeneration efficiency of 25 months sustained in vitro culture of Allium ampeloprassum L. was maintained through multiple shoot proliferation and root growth. No morphological variation was detected between the plantlets regenerated from initial and long-term shoot tip culture or with their mother, meaning a high likelihood of their clonal fidelity. Detection of genetic integrity for in vitro regenerated clones was carried out using 10 ISSR primers among which 4 primers produced a total number of 336 distinct and scorable bands with an average of 7 bands per primers where the other 6 primers were not reproducible. The ISSR primers base on AG motif and 3 ' anchoring produced more number of consistent bands. All monomorphic bands in the ISSR assay both for primary culture as well long term culture in vitro ascertained to a great extent their genetic integrity through their partial genetic coverage. © 2010 Asian Network for Scientific Information.

Gantait S.,Instrumentation and Environmental Science | Mandal N.,Instrumentation and Environmental Science | Bhattacharyya S.,Bidhan Chandra Krishi Viswavidyalaya | Das P.K.,Bidhan Chandra Krishi Viswavidyalaya
Biotechnology | Year: 2010

Aloe (Aloe vera L., Family Liliaceae), with proven multiple medicinal values finds favour readily in Ayurevdic applications, is facing a serious threat to its population as well as biodiversity due to its popularly harvested aloe leaves by the local communities and herbal medicine vendors. To succeed in dealing with the specified problem along with the facilitation of the mass production of commercial level, constant supply of quality propagules can be possible through conservation of propagules in vitro. The present study is thus concerned with in vitro conservation of multiple shoot culture of aloe to achieve unbroken supply of propagules maintaining their genetic purity. Rhizomatous stem explants result multiple bud break in MS plus 0.25 mg L -1 of NAA and 1.5 mg L-1 of BAP. Separated shoot buds further result in shoot multiplication and proliferation on MS with 2.5 mg L-1 of BAP. In vitro generated multiple shoots were split into individual shoot and subcultured for further multiplication in a sustainable. Five subcultures were performed at an extended 5 month interval over a period of 25 months in the same medium. Plantlets regenerated after 1 st subculture and plantlets from 5th subculture showed no significant difference in the phenotypic response. Genetic integrity of in vitro clones was tested using ISSR primers. All monomorphic bands in the ISSR assay, both for primary culture as well long term culture, ascertained their genetic integrity to a great extent. © 2010 Asian Network for Scientific Information.

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