El Puerto de Santa María, Spain
El Puerto de Santa María, Spain

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Assuncao P.,Instituto Tecnologico Of Canarias Itc | Wolf M.,University of Würzburg | Buchheim M.A.,University of Tulsa | de la Jara A.,Instituto Tecnologico Of Canarias Itc | And 3 more authors.
Phycological Research | Year: 2013

We analyzed the ITS2 primary and secondary structure (including Compensatory Base Changes (CBCs)) of 17 new Dunaliella strains (11 D.viridis, two D.tertiolecta, and four Dunaliella sp.), and compared these with other Dunaliella sequences available from the ITS2 database to circumscribe their taxonomic position. The ITS2 primary and secondary structure analysis positioned the majority of D.viridis strains in four main clades, showing that D.viridis is polyphyletic. The detection of at least one CBC among these clades strongly suggests that they could correspond to different biological species. Unexpectedly, while D.viridis var. euchlora (CCAP19/21) was positioned within the subgenus Dunaliella, D.viridis var. palmelloides (CCAP11/34) was positioned clearly outside this subgenus, suggesting that this taxon may not be properly placed in Dunaliella. Furthermore, the detection of at least three compensatory base changes (CBCs) between D.viridis var. palmelloides (CCAP11/34) and the other strains analyzed, confirm that this strain is a different species. For these reasons we propose re-naming D.viridis var. palmelloides (CCAP11/34) to incertae sedis, and D.viridis var. euchlora (CCAP19/21) to Dunaliella sp. Therefore, the ITS2 primary and secondary structure data suggest a taxonomic re-structuring of D.viridis. © 2013 Japanese Society of Phycology.

Raluy R.G.,Instituto Tecnologico Of Canarias Itc | Schwantes R.,Fraunhofer Institute for Solar Energy Systems | Subiela V.J.,Instituto Tecnologico Of Canarias Itc | Penate B.,Instituto Tecnologico Of Canarias Itc | And 2 more authors.
Desalination | Year: 2012

Desalination is a technology with a high energy demand, mainly supplied by fossil sources. Therefore, solar energy is a real alternative, as it supplies virtually unlimited energy. Solar desalination could be an ideal solution for isolated areas which receive high solar irradiation per day. Solar Membrane Distillation (MD) is a developing technology particularly interesting for small supplies, isolated populations and autonomous systems. As it is a low temperature operation process, MD is very suitable for integration with thermal solar systems and/or waste heat recovery units; in contrast to other desalination technologies, only a coarse filtration of the water is required as pretreatment. However, the commercial availability is currently quite limited. This paper presents the promising 5-year experience and data analysis of a solar compact MD demonstration plant installed in the facilities of the Instituto Tecnológico de Canarias (ITC) in Playa de Pozo Izquierdo (Gran Canary Island-Spain).The unit was designed and installed at the end of 2004 as part of a co-funded FP6 DGTREN research project called 'Development of stand-alone, solar thermally driven and PV-supplied desalination systems based on innovative membrane distillation' (MEMDIS) and has been continuously tested within the FP7 EU co-funded project called 'MEmbrane DIstillation in Remote AreaS - MEDIRAS'. © 2012 Elsevier B.V.

Assuncao P.,Instituto Tecnologico Of Canarias Itc | Jaen-Molina R.,University of Las Palmas de Gran Canaria | Caujape-Castells J.,University of Las Palmas de Gran Canaria | de la Jara A.,Instituto Tecnologico Of Canarias Itc | And 3 more authors.
Journal of Applied Phycology | Year: 2011

Dunaliella acidophila is one of the most extreme acidophiles on earth and is able to survive in highly acidic habitats. This characteristic has made this organism the universal model for the study of abiotic stress. Although D. acidophila is currently circumscribed to the subgenus Pascheria within Dunaliella Teodoresco (Chlorophyceae), its taxonomic position has stirred controversy. The comparison of D. acidophila CCAP19/35 internal transcribed spacers (including ITS2 secondary structure analysis) and RuBisCo large subunit (rbcL) sequences with other Dunaliella species confirms that D. acidophila should maintain its phylogenetic position within the genus Dunaliella, suggesting its inclusion within the subgenus Dunaliella. Furthermore, the ITS1 and ITS2 data revealed that D. acidophila was highly divergent from the other freshwater species assessed, D. lateralis, with which it barely shares a 56.8% similarity. © 2011 Springer Science+Business Media B.V.

Penate B.,Instituto Tecnologico Of Canarias Itc | Subiela V.J.,Instituto Tecnologico Of Canarias Itc | Vega F.,Instituto Tecnologico Of Canarias Itc | Castellano F.,Instituto Tecnologico Of Canarias Itc | And 2 more authors.
Desalination and Water Treatment | Year: 2015

Abstract: Ksar Ghilène is a 300 inhabitant isolated village located in the Sahara desert at the South of Tunisia and belonging to the region of Kébili. Due to the particular location of this site, there was no local fresh water source before 2006 and this community had been depending on the external water supply, transported by trucks. The 10.5 kWp PV-powered RO desalination system (based on the international patent DESSOL®) with a nominal water capacity of 50 m3/d was commissioned in June 2006 and currently in operation. The whole project was developed by Instituto Tecnológico de Canarias within the framework of the Spanish–Tunisian cooperation, thanks to the economic support of the Spanish cooperation and the Canary Islands cooperation. This paper presents and assesses the operation data and lessons learnt with the whole system for the period 2006–2013. © 2014 Balaban Desalination Publications. All rights reserved.

We used an ITS2 primary and secondary structure and Compensatory Base Changes (CBCs) analyses on new French and Spanish Dunallela salina strains to investigate their phylogenetic position and taxonomic status within the genus Dunaliella. Our analyses show a great diversity within D. salina (with only some clades not statistically supported) and reveal considerable genetic diversity and structure within Dunaliella, although the CBC analysis did not bolster the existence of different biological groups within this taxon. The ITS2 sequences of the new Spanish and French D. salina strains were very similar except for two of them: ITC5105 Janubio from Spain and ITC5119 from France. Although the Spanish one had a unique ITS2 sequence profile and the phylogenetic tree indicates that this strain can represent a new species, this hypothesis was not confirmed by CBCs, and clarification of its taxonomic status requires further investigation with new data. Overall, the use of CBCs to define species boundaries within Dunaliella was not conclusive in some cases, and the ITS2 region does not contain a geographical signal overall.

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