Instituto Tecnologico Of Canarias

Las Palmas de Gran Canaria, Spain

Instituto Tecnologico Of Canarias

Las Palmas de Gran Canaria, Spain

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De Los Reyes C.,University of Cádiz | Avila-Roman J.,University of Seville | Ortega M.J.,University of Cádiz | De La Jara A.,Instituto Tecnologico Of Canarias | And 3 more authors.
Phytochemistry | Year: 2014

The chemical study of the microalgae Chlamydomonas debaryana and Nannochloropsis gaditana has led to the isolation of oxylipins. The samples of C. debaryana have yielded the compounds (4Z,7Z,9E,11S,13Z)-11-hydroxyhexadeca-4, 7,9,13-tetraenoic acid (1), (4Z,7E,9E,13Z)-11-hydroxyhexadeca-4,7,9,13- tetraenoic acid (2), (4Z,6E,10Z,13Z)-8-hydroxyhexadeca-4,6,10,13-tetraenoic acid (3), (4Z,8E,10Z,13Z)-7-hydroxyhexadeca-4,8,10,13-tetraenoic acid (4), and (5E,7Z,10Z,13Z)-4-hydroxyhexadeca-5,7,10,13-tetraenoic acid (5), which are derived from the fatty acid 16:4Δ4,7,10,13 together with the compound (5Z,9Z,11E,15Z)-13-hydroxyoctadeca-5,9,11,15-tetraenoic acid (7) derived from coniferonic acid (18:4Δ5,9,12,15). In addition, the known polyunsaturated hydroxy acids 11-HHT (6), (5Z,9Z,11E)-13- hydroxyoctadeca-5,9,11-trienoic acid (8), (13S)-HOTE (9), (9E,11E,15Z)-13- hydroxyoctadeca-9,11,15-trienoic acid (10), 9-HOTE (11), 12-HOTE (12), 16-HOTE (13) and (13S)-HODE (14) have also been obtained. The chemical study of N. gaditana has led to the isolation of the hydroxy acid (15S)-HEPE (15) derived from EPA (20:5Δ5,8,11,14,17). The structures of the isolated compounds were established by spectroscopic means. The optical activity displayed by oxylipins 1, 2, 6, 7, 9, 10, 14, and 15 suggests the occurrence of LOX-mediated pathways in C. debaryana and N. gaditana. In anti-inflammatory assays, all the tested compounds inhibited the TNF-α production in LPS-stimulated THP-1 macrophages. The most active oxylipin was the C-16 hydroxy acid 1, which at 25 μM caused a 60% decrease of the TNF-α level. © 2014 Elsevier Ltd. All rights reserved.


Mendoza H.,Instituto Tecnologico Of Canarias | Carmona L.,Instituto Tecnologico Of Canarias | Assuncao P.,Instituto Tecnologico Of Canarias | Freijanes K.,Instituto Tecnologico Of Canarias | And 3 more authors.
Bioresource Technology | Year: 2015

The lipid extractability of 14 microalgae species and strains was assessed using organic solvents (methanol and chloroform). The high variability detected indicated the potential for applying this parameter as an additional criterion for microalgae screening in industrial processes such as biofuel production from microalgae. Species without cell walls presented higher extractability than species with cell walls. Analysis of cell integrity by flow cytometry and staining with propidium iodide showed a significant correlation between higher resistance to the physical treatments of cell rupture by sonication and the lipid extractability of the microalgae. The results highlight the cell wall as a determining factor in the inter- and intraspecific variability in lipid extraction treatments. © 2015.


Loague K.,Stanford University | Blanke J.S.,RMC Water and Environment | Mills M.B.,Stanford University | Diaz-Diaz R.,Instituto Tecnologico Of Canarias | Corwin D.L.,U.S. Department of Agriculture
Journal of Environmental Quality | Year: 2012

Precious groundwater resources across the United States have been contaminated due to decades-long nonpoint-source applications of agricultural chemicals. Assessing the impact of past, ongoing, and future chemical applications for large-scale agriculture operations is timely for designing best-management practices to prevent subsurface pollution. Presented here are the results from a series of regional-scale vulnerability assessments for the San Joaquin Valley (SJV). Two relatively simple indices, the retardation and attenuation factors, are used to estimate near-surface vulnerabilities based on the chemical properties of 32 pesticides and the variability of both soil characteristics and recharge rates across the SJV. Th e uncertainties inherit to these assessments, derived from the uncertainties within the chemical and soil data bases, are estimated using fi rst-order analyses. Th e results are used to screen and rank the chemicals based on mobility and leaching potential, without and with consideration of data-related uncertainties. Chemicals of historic high visibility in the SJV (e.g., atrazine, DBCP [dibromochloropropane], ethylene dibromide, and simazine) are ranked in the top half of those considered. Vulnerability maps generated for atrazine and DBCP, featured for their legacy status inthe study area, clearly illustrate variations within and across the assessments. For example, the leaching potential is greater for DBCP than for atrazine, the leaching potential for DBCP is greater for the spatially variable recharge values than for the average recharge rate, and the leaching potentials for both DBCP and atrazine are greater for the annual recharge estimates than for the monthly recharge estimates. Th e data-related uncertainties identifi ed in this study can be signifi cant, targeting opportunities for improving future vulnerability assessments. © 2012 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.


Guzman H.M.,Instituto Tecnologico Of Canarias | de la Valido A.J.,Instituto Tecnologico Of Canarias | Duarte L.C.,Instituto Tecnologico Of Canarias | Presmanes K.F.,Instituto Tecnologico Of Canarias
Journal of Applied Phycology | Year: 2011

Relative polyunsaturated fatty acid content and unsaturation index are very important composition variables in the use of microalgae both for animal and human nutrition and biofuel production. A readily available technique to rapidly and inexpensively estimate relative fatty acid composition is very important for mass screening of new strains for the production of different types of oil. This study demonstrates the validity of Nile Red staining and flow cytometry for quick estimation of unsaturation index and relative fatty acid content in microalgae. Nile Red staining allows polar and neutral lipid contents to be estimated, and in this study a significant correlation was observed between polar/neutral ratio and fatty acid content in the species studied, corresponding to higher polyunsaturated fatty acid content in the polar lipid fraction of microalgae. This technique enables quick estimation of relative polyunsaturated fatty acid content and interspecific variation, as well as variations caused by culture conditions. In the species studied, most variability in fatty acid composition was due to variation in monounsaturated and polyunsaturated fatty acids, with less variation observed in saturated fatty acid content. © 2010 Springer Science+Business Media B.V.


Mendoza Guzman H.,Instituto Tecnologico Of Canarias | de la Jara Valido A.,Instituto Tecnologico Of Canarias | Freijanes Presmanes K.,Instituto Tecnologico Of Canarias | Carmona Duarte L.,Instituto Tecnologico Of Canarias
Journal of Applied Phycology | Year: 2012

This study shows how relative fatty acid content and unsaturation index of Dunaliella salina strains can be estimated rapidly by analysis with flow cytometry and Nile Red staining. This technique is valid for analysing intraspecific variability and shows no significant distortion phenomena, such as variability in the staining of strains not associated with different fatty acid composition. These phenomena have been related to variations in the permeability of the cell membrane and cell wall to Nile Red in a number of microalgae strains and species. The saturated fatty acids/monounsaturated fatty acids/polyunsaturated fatty acids ratio was estimated indirectly from the relative polar and neutral lipid composition. High intraspecific variability was noted in the fatty acid composition of D. salina, which makes it an ideal species for conducting screening tests of new strains for oil production, using the strategic advantages associated with this species such as the possibility of mass culturing in inexpensive, open systems and high biomass processability due to its lack of cell wall. © 2011 Springer Science+Business Media B.V.


Rodriguez-Rodriguez A.,University of Las Palmas de Gran Canaria | Dominguez F.Q.-.,University of Las Palmas de Gran Canaria | Martel-Rodriguez G.,Instituto Tecnologico Of Canarias | Marquez-Marfil M.,Instituto Tecnologico Of Canarias
Iberian Conference on Information Systems and Technologies, CISTI | Year: 2016

This work describes a practical case for an assessment knowledge-based system (KBS). We consider that problem solving methods play an essential role in the development process of a KBS; not only to describe the inference process, but also to guide many development activities, such as facilitating a common understanding between domain experts and knowledge engineers, scheduling knowledge acquisition sessions, or describing key information structures. Furthermore, we think that, in order to develop a successful KBS, we must integrate it in an Information System which facilitates the understanding of the KBS results. © 2016 AISTI.


Ould Mohamedou E.,University of Nouakchott | Ould Mohamedou E.,University of Nantes | Penate Suarez D.B.,Instituto Tecnologico Of Canarias | Vince F.,Ecole Polytechnique Federale de Lausanne | And 2 more authors.
Desalination | Year: 2010

The overall objective of the present work was to estimate by membrane autopsy the level of performances degradation of old reverse osmosis (RO) membranes/modules in order to envisage their reuse. A mechanistic approach using the Spiegler-Kedem-Katchalsky model helps us to observe that the old RO membrane acquired a convective mass transfer. We defined a novel Peclet number (denoted Pe′) usable to distinguish between diffusional and convective mass transfer. We observed that for the old membrane Pe′ numbers are always higher than 1. Furthermore, SEM/AFM and EDX experiments show crystals and bacteria particles as fouling agents, roughness increase for the old membrane (from 73 to 220 nm) due to foulants deposition. EDX experiments demonstrated that FexOy crystals are dominant. SP measurements help us to observe a displacement of the isoelectric point (IEP) for the virgin membrane from 2.7 ± 0.3 to 4.6 ± 0.3 in comparison to the old one showing chemical modifications of the inner active layer suspected due to biofouling residuals. In the last part and for the first time, module materials reuse occurred in the place of their incineration as geotextile in proper home garden. Crown Copyright © 2009.


Sanchez Rodriguez A.,Instituto Tecnologico Of Canarias | Rodrigo Sanz M.,Instituto Tecnologico Of Canarias | Betancort Rodriguez J.R.,Instituto Tecnologico Of Canarias
Chemosphere | Year: 2015

Due to the growing concern about human health effects of ultraviolet (UV) radiation, the use of UV filters has increased in recent decades. Unfortunately, some common UV filters are bioaccumulated in aquatic organisms and show a potential for estrogenic activity. The aim of the present study is to determine the presence of some UV filters in the coastal waters of six beaches around Gran Canaria Island as consequence of recreational seaside activities. Eight commonly used UV filters: benzophenone-3 (BP-3), octocrylene (OC), octyl-dimethyl-PABA (OD-PABA), ethylhexyl methoxy cinnamate (EHMC), homosalate (HMS), butyl methoxydibenzoyl methane (BMDBM), 4-methylbenzylidene camphor (4-MBC) and diethylamino hydroxybenzoyl hexyl benzoate (DHHB), were monitored and, with the exception of OD-PABA, all were detected in the samples collected. 99% of the samples showed some UV filters and concentration levels reached up to 3316.7. ng/L for BP-3. Environmental risk assessment (ERA) approach showed risk quotients (RQ) higher than 10, which means that there is a significant potential for adverse effects, for 4-MBC and EHMC for those samples with highest levels of UV filters. © 2015 Elsevier Ltd.


Guzman H.M.,Instituto Tecnologico Of Canarias | de la Jara Valido A.,Instituto Tecnologico Of Canarias | Duarte L.C.,Instituto Tecnologico Of Canarias | Presmanes K.F.,Instituto Tecnologico Of Canarias
Aquaculture International | Year: 2010

The present work shows the possibility of determining variations in the lipid composition in Tetraselmis suecica under different conditions of culture by means of flow cytometry in cells marked with Nile Red (NR). A significant correlation was observed between the cellular contents in polar and neutral lipids and the cytometric signal of the marked cells. Likewise, there was a significant correlation between the ratio of polar and neutral lipids, estimated by cytometry, and the relative composition of polyunsaturated fatty acids (PUFAs) in Tetraselmis, which corresponded to the greater content of PUFAs detected in the polar lipid fraction of this microalgae. This relationship between the polar/neutral ratio and the relative contents of PUFAs, together with the flow cytometry and the marking by means of NR, would make it possible to have an effective indicator of the abundance of PUFAs in Tetraselmis, as well as the development of techniques of massive screening of strains which are hyperproductive of PUFAs and of rapid checking of the variations in lipid composition in response to cultivation conditions, which are much simpler and more rapid than traditional techniques. © 2009 Springer Science+Business Media B.V.


PubMed | Instituto Tecnologico Of Canarias
Type: | Journal: Chemosphere | Year: 2015

Due to the growing concern about human health effects of ultraviolet (UV) radiation, the use of UV filters has increased in recent decades. Unfortunately, some common UV filters are bioaccumulated in aquatic organisms and show a potential for estrogenic activity. The aim of the present study is to determine the presence of some UV filters in the coastal waters of six beaches around Gran Canaria Island as consequence of recreational seaside activities. Eight commonly used UV filters: benzophenone-3 (BP-3), octocrylene (OC), octyl-dimethyl-PABA (OD-PABA), ethylhexyl methoxy cinnamate (EHMC), homosalate (HMS), butyl methoxydibenzoyl methane (BMDBM), 4-methylbenzylidene camphor (4-MBC) and diethylamino hydroxybenzoyl hexyl benzoate (DHHB), were monitored and, with the exception of OD-PABA, all were detected in the samples collected. 99% of the samples showed some UV filters and concentration levels reached up to 3316.7 ng/L for BP-3. Environmental risk assessment (ERA) approach showed risk quotients (RQ) higher than 10, which means that there is a significant potential for adverse effects, for 4-MBC and EHMC for those samples with highest levels of UV filters.

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