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Salinas Y.,Unidad University | Salinas Y.,Polytechnic University of Valencia | Salinas Y.,CIBER ISCIII | Climent E.,Unidad University | And 20 more authors.
Chemical Communications

Silica nanoparticles containing polyamines and thiol groups have been used as probes for the selective detection of Tetryl. © 2011 The Royal Society of Chemistry. Source

Martinez I.,Technical University of Madrid | Casas P.A.,Instituto Tecnologico la Maranosa
Brazilian Journal of Chemical Engineering

A simple analytical model is presented for the time-evolution of dissolved CO 2 concentration in a batch water column. The application in mind is the removal of the CO 2 produced by the propulsion plant of a submarine cruising underwater. The model is based on a constant number of constant size CO 2 bubbles within the water column, with uniform gas injection through a porous membrane at the bottom, and bubbles flowing upwards by their own buoyancy through a short water column (the exhaust gas is recirculated). Analytical results are validated with experimental data found in the literature and the advantages of this simple analytical model pinpointed. Source

Paauw A.,TNO | Trip H.,TNO | Niemcewicz M.,Military Institute of Hygiene and Epidemiology | Sellek R.,Instituto Tecnologico la Maranosa | And 7 more authors.
BMC Microbiology

Cholera is an acute diarrheal disease caused by Vibrio cholerae. Outbreaks are caused by a genetically homogenous group of strains from serogroup O1 or O139 that are able to produce the cholera toxin. Rapid detection and identification of these epidemic strains is essential for an effective response to cholera outbreaks. Results: The use of ferulic acid as a matrix in a new MALDI-TOF MS assay increased the measurable mass range of existing MALDI-TOF MS protocols for bacterial identification. The assay enabled rapid discrimination between epidemic V. cholerae O1/O139 strains and other less pathogenic V. cholerae strains. OmpU, an outer membrane protein whose amino acid sequence is highly conserved among epidemic strains of V. cholerae, appeared as a discriminatory marker in the novel MALDI-TOF MS assay. Conclusions: The extended mass range of MALDI-TOF MS measurements obtained by using ferulic acid improved the screening for biomarkers in complex protein mixtures. Differences in the mass of abundant homologous proteins due to variation in amino acid sequences can rapidly be examined in multiple samples. Here, a rapid MALDI-TOF MS assay was developed that could discriminate between epidemic O1/O139 strains and other less pathogenic V. cholerae strains based on differences in mass of the OmpU protein. It appeared that the amino acid sequence of OmpU from epidemic V. cholerae O1/O139 strains is unique and highly conserved. © 2014 Paauw et al.; licensee BioMed Central Ltd. Source

Riveraa C.,Ingenieria y Servicios Aeroespaciales | Riveraa C.,Instituto Tecnologico la Maranosa
Journal of Applied Physics

The determination of the electromechanical properties of materials for a parallel-plate capacitor structure is affected by the electrostatic force between their electrodes. The corrections resulting from this electric-field-induced stress are usually assumed to be linked to the quadratic dependence of the strain on the electric field (electrostriction). Here we show by calculations based on thermodynamic grounds for this simple structure that the effect of the electrostatic force on piezoelectric materials can lead to both quadratic and linear corrections through the combination of the piezoelectric coupling and spontaneous polarization. The case of GaN-based capacitor nanoscale structures is presented taking into account the boundary conditions imposed as well as the effect of geometry. The results in this example point to corrections in the piezoelectric and electrostrictive coefficients higher than 0.3 pm V- 1 and 2.6×10- 22 m 2 V- 2, respectively. © 2011 American Institute of Physics. Source

Sellek R.E.,Instituto Tecnologico la Maranosa | Niemcewicz M.,Military Institute of Hygiene and Epidemiology | Olsen J.S.,Norwegian Defence Research Establishment Instituttveien | Bassy O.,Instituto Tecnologico la Maranosa | And 6 more authors.
Epidemiology and Infection

A total of 111 clinical and environmental O1, O139 and non-O1/O139 Vibrio cholerae strains isolated between 1978 and 2008 from different geographical areas were typed using a combination of methods: antibiotic susceptibility, biochemical test, serogroup, serotype, biotype, sequences containing variable numbers of tandem repeats (VNTRs) and virulence genes ctxA and tcpA amplification. As a result of the performed typing work, the strains were organized into four clusters: cluster A1 included clinical O1 Ogawa and O139 serogroup strains (ctxA + and tcpA +); cluster A2 included clinical non-O1/O139 strains (ctxA and tcpA), as well as environmental O1 Inaba and non-O1/O139 strains (ctxA and tcpA/tcpA +); cluster B1 contained two clinical O1 strains and environmental non-O1/O139 strains (ctxA and tcpA +/tcpA a); cluster B2 contained clinical O1 Inaba and Ogawa strains (ctxA + and tcpA +). The results of this work illustrate the advantage of combining several typing methods to discriminate between clinical and environmental V. cholerae strains. © 2011 Cambridge University Press. Source

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