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Vilafranca del Penedès, Spain

Spasovski G.,University of Macedonia | Cochat P.,University of Lyon | Claas F.H.J.,Leiden University | Pascual J.,Hospital Del Mar Barcelona | And 15 more authors.
BANTAO Journal | Year: 2014

The Clinical Practice Guideline on evaluation of the kidney donor and transplant recipient was developed following a rigorous methodological approach aiming to provide information and aid decision-making to the transplant professionals. Thus, this document should help caregivers to improve the quality of care they deliver to patients with no intention it is defined as a standard of care. In this short version of the guidelines we present 112 statements about the evaluation of the kidney transplant candidate as well as the potential deceased and living donor, the immunological work-up of kidney donors and recipients and the perioperative recipient care. The extended version of the guidelines with methods, rationale and references is published in Nephrol Dial Transplant (2013) 28: i1-i71; doi: 10.1093/ndt/gft218 and can be downloaded freely from http://www.oxfordjournals.- org/ our-journals/ndt/era-edta.html. Source


Naves-Diaz M.,University of Oviedo | Naves-Diaz M.,Instituto Reina Sofia Of Investigacion | Carrillo-Lopez N.,University of Oviedo | Carrillo-Lopez N.,Instituto Reina Sofia Of Investigacion | And 9 more authors.
Menopause | Year: 2010

Objective: The aim of this study was to evaluate the effect of 17β-estradiol, raloxifene, and 1-α,25-dihydroxycholecalciferol or calcitriol on bone and lipid metabolism in chronic kidney disease and estrogen insufficiency. Methods: Six-month-old female Sprague-Dawley rats (n = 48) were ovariectomized and nephrectomized (seven eighths). One week after surgery, the rats were divided into six groups and treated with (1) placebo, (2) 17β-estradiol 10 μg kg day, (3) raloxifene 1 mg kg day, (4) calcitriol 10 ng kg day, (5) 17β-estradiol + calcitriol, and (6) raloxifene + calcitriol. A group of untreated animals with chronic kidney disease and normal ovarian function was used as a control group (n = 5). The rats were killed after 8 weeks of treatment. Blood samples were drawn for serum analyses; the right tibia was removed to perform histomorphometric analyses, uteri were used as tissue markers of estrogen replacement, and paraffin-embedded sections of the uterus and the fourth breast were used for histopathologic evaluation. Results: Raloxifene, alone or combined with calcitriol, and 17β-estradiol combined with calcitriol significantly diminished total cholesterol level compared with placebo. Qualitative histological and histomorphometric analyses showed that both the single treatments and their combinations were able to increase the trabecular connectivity compared with placebo. The less beneficial results were obtained with 17β-estradiol alone, whereas the more beneficial results were obtained with the combined treatments, particularly with raloxifene and calcitriol. Conclusions: In summary, this experimental study demonstrates the advantages of replacing both hormonal deficiencies together. The combination of calcitriol and raloxifene, a selective estrogen receptor modulator, showed a better lipid, uterus, and bone profile. © 2010 by The North American Menopause Society. Source


Arias M.S.,Instituto Reina Sofia Of Investigacion | Carcedo A.G.,Instituto Reina Sofia Of Investigacion | de la Torre C.S.,Instituto Reina Sofia Of Investigacion | Garcia M.R.,Instituto Reina Sofia Of Investigacion | And 5 more authors.
Revista de la Sociedad Espanola de Enfermeria Nefrologica | Year: 2010

The aim of the study was to analyse the risk of fracture calculated using the FRAX® tool and its determinants in patients who have received a kidney transplant referred to us for control of bone repercussions. A total of 113 patients were studied, 42 men and 71 women with a functioning transplanted kidney, who completed a general survey. A bone densitometry test of the PA lumbar spine (L2-L4) and hip (femoral neck and total hip) was carried out on all patients using a Hologic QDR 1000® densitometer. With the data obtained from the survey, the risk of fracture was calculated using the FRAX® tool. Source


Abramowicz D.,University of Antwerp | Cochat P.,University of Lyon | Claas F.H.J.,Leiden University | Pascual J.,Hospital Del Mar Barcelona | And 15 more authors.
Nephrology Dialysis Transplantation | Year: 2015

The European Best Practice Guideline group (EBPG) issued guidelines on the evaluation and selection of kidney donor and kidney transplant candidates, as well as post-transplant recipient care, in the year 2000 and 2002. The new European Renal Best Practice board decided in 2009 that these guidelines needed updating. In order to avoid duplication of efforts with kidney disease improving global outcomes, which published in 2009 clinical practice guidelines on the post-transplant care of kidney transplant recipients, we did not address these issues in the present guidelines. The guideline was developed following a rigorous methodological approach: (i) identification of clinical questions, (ii) prioritization of questions, (iii) systematic literature review and critical appraisal of available evidence and (iv) formulation of recommendations and grading according to Grades of Recommendation Assessment, Development, and Evaluation (GRADE). The strength of each recommendation is rated 1 or 2, with 1 being a 'We recommend' statement, and 2 being a 'We suggest' statement. In addition, each statement is assigned an overall grade for the quality of evidence: A (high), B (moderate), C (low) or D (very low). The guideline makes recommendations for the evaluation of the kidney transplant candidate as well as the potential deceased and living donor, the immunological work-up of kidney donors and recipients and perioperative recipient care. All together, the work group issued 112 statements. There were 51 (45%) recommendations graded '1', 18 (16%) were graded '2' and 43 (38%) statements were not graded. There were 0 (0%) recommendations graded '1A', 15 (13%) were '1B', 19 (17%) '1C' and 17 (15%) '1D'. None (0%) were graded '2A', 1 (0.9%) was '2B', 8 (7%) were '2C' and 9 (8%) '2D'. Limitations of the evidence, especially the lack of definitive clinical outcome trials, are discussed and suggestions are provided for future research. We present here the complete recommendations about the evaluation of the kidney transplant candidate as well as the potential deceased and living donor, the immunological work-up of kidney donors and recipients and the perioperative recipient care. We hope that this document will help caregivers to improve the quality of care they deliver to patients. The full version with methods, rationale and references is published in Nephrol Dial Transplant (2013) 28: i1-i71; doi: 10.1093/ndt/gft218 and can be downloaded freely from http://www.oxfordjournals.org/our-journals/ndt/era-edta.html. © The Author 2014. Published by Oxford University Press. Source


Serrano I.,University of Alcala | Serrano I.,Cancer Research Center | De Frutos S.,University of Alcala | De Frutos S.,Instituto Reina Sofia Of Investigacion | And 10 more authors.
Cardiovascular Research | Year: 2013

Aims Integrin-linked kinase (ILK) regulates proliferation, differentiation, cell adhesion, and motility in many cell types and has been related to cancer progression, fibrosis, and vascular diseases.We designed the present study to directly explore the effect of ILK deletion on the regulation of vascular tone through the soluble guanylate cyclase (sGC) /protein kinase G (PKG) pathway in healthy adult mice. Methods and results Experiments were carried out using a tamoxifen-inducible CRE-LOXsystem to conditionally delete the ILK gene in adult mice. Mice lacking ILK expression (cKO) presented increased vascular content and increased activity of sGC and PKG, resulting in a more intense vasodilatory response to a single dose of a nitric oxide (NO) donor [sodium nitroprusside (SNP)] or PKG agonist [8-bromoguanosine 3′, 5′-cyclic monophosphate sodium salt (8-Br)]. Five minutes after SNP or 8-Br administration the reduction in the systolic arterial pressure was enhanced in cKO mice (SNP WT:-7.4±1.2 mmHG; SNP cKO:-14.0±2.5; 8-Br WT:-2.9±1.5 mmHG; 8-Br cKO:-10.0±3.4 mmHG). ILK deletion restored the vascular response to SNP after chronic oral nitrite administration. In addition, ILK deletion also increased hypotensiveSNPeffect in angiotensin II-treated animals, suggesting a role for ILK in basal and pathological states. Conclusion Deletion of ILK in adult animals increased the vascular response to NO. These findings show, for the first time, a requirement for ILK in regulating sGC-PKG expression in vivo. © The Author 2013. Source

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