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Kodad O.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Jfelipe J.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Socias I Company R.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Sanchez A.,Instituto Of Tecnologia Quimica E Biologica Itqb | Oliveira M.M.,University of Lisbon
Acta Horticulturae | Year: 2011

The finding of new self-compatible cultivars in local population is a breeder's objective in order to increase the sources of self-compatibility for almond (Prunus amygdalus Batsch) breeding programmes. 'AS-1', a local Spanish selection, was considered to be self-compatible according to its self pollen tube growth and was incorporated in the almond breeding programme of Zaragoza as a source for this trait. However, both pollen tube growth and fruit set after self-pollination have shown that this selection is self-incompatible. The PCR analysis using specific and consensus primers revealed that its genotype is S 8S 12, where both alleles control selfincompatibility and do not confer self-compatibility in almond. The field crosses of 'AS-1' and 'Marcona Flota', of the same S genotype, were incompatible, confirming the S genotype of 'AS-1' determined by PCR analysis. The sequencing of the SFB S haplotype showed that 'AS-1' presents SFB 8 and SFB 12. Moreover, the absence of any notable deletion or insertion upstream from the HVa and HVb regions in the sequence of the SFB 8 and SFB 12 S-haplotypes confirm their identity. All these results, including pollination tests, PCR analysis, and cloning and sequencing of the S alleles of 'AS-1', indicate that this local selection is self-incompatible. Source


Kodad O.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Alonso J.M.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Fernandez I Marti A.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Socias I Company R.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | And 2 more authors.
Acta Horticulturae | Year: 2011

The identity of the pistil (S-RNase) and the pollen (SFB protein) components of the S allele presumably conferring self-compatibility in almond (Sf) were confirmed in 'Ponç', a local Spanish almond cultivar. The 'Ponç' phenotype was confirmed as self-incompatible by several tests, including self pollen tube growth, fruit set after self-pollination, and fruit set in bagged branches. Due to its phenological expression, the Sf allele of 'Ponç' was called Sfa (active Sf-allele). The lack of any alteration in the C1-C5 regions of the Sfa-allele of 'Ponç' when compared with the Sf-allele of self-compatible cultivars indicates that the origin of selfcompatibility in almond is not due to any alteration in those regions. The presence of cultivars possessing an active Sf-allele indicates that the Sf gene may not be the exclusive source of self-compatibility in almond and that probably an alteration upstream of the coding sequence or some modifier genes must contribute to its expression. Source


Kodad O.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Alonso J.M.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Fernandez i Marti A.,CSIC - Centro de Investigacion y Tecnologia Agroalimentaria | Oliveira M.M.,Instituto Of Tecnologia Quimica E Biologica Itqb | And 2 more authors.
Scientia Horticulturae | Year: 2010

The S-allele characterisation of 'Alzina' and 'Garondès', two local almond cultivars from the island of Majorca, by a multidimensional approach has allowed the confirmation of the presence of the Sf-allele and the identification of a new allele not previously described in almond, S36. When these cultivars were phenotypically evaluated, both showed a self-incompatible phenotype and were cross-incompatible, as assessed by artificial self- and cross-pollinations and fruit sets after field pollinations, confirming that their Sf-allele is in its active form, Sfa. Thus a new CGI group in almond is proposed and named XXVIII. These results confirm the wide diversity of S-alleles in almond both at genotypic and phenotypic levels, as well as their similarity with the S-alleles from other close Prunus species. This similarity suggests the possibility of allele introgression between species or allele identity by descent from a common ancestor. © 2009 Elsevier B.V. All rights reserved. Source


Santos A.M.,Instituto Of Tecnologia Quimica E Biologica Itqb | Oliver M.J.,University of Missouri | Sanchez A.M.,Instituto Of Tecnologia Quimica E Biologica Itqb | Oliveira M.M.,Instituto Of Tecnologia Quimica E Biologica Itqb
In Vitro Cellular and Developmental Biology - Plant | Year: 2012

The transcription factor encoded by the gene Knotted1 is a nuclear homeodomain protein, regulating meristematic cells at the shoot apical meristem. It has been demonstrated that Knotted1 (KN1) expression specifies stem cell fate in adventitious shoot induction in herbaceous plants. This gene may thus potentially identify the initiation of meristem development in adventitious shoot induction in difficult-to-regenerate plants such as Prunoideae. We isolated an almond (Prunus dulcis Mill.) KN1-type gene using degenerate primers targeting the most conserved regions of Knotted1 gene. The 5′ and 3′ ends of the isolated sequence were obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR), and the gene was named P. dulcis Knotted1-like (PdKn1). PdKn1 transcripts were detected by reverse transcription (RT)-PCR mainly in shoot apical and axillary meristems. The RT-PCR and RT-quantitative PCR PdKn1 expression in almond leaf explants was found to anticipate the organization of adventitious shoot meristems. Apricot RNA isolated from induced leaf explants cross-hybridized with the almond probe PdKn1 in Northern blotting. We suggest that almond PdKn1 may be a useful marker to study the adventitious regeneration system by revealing the potential organogenic conditions, not only in almond but also in other Prunoideae. © 2011 The Society for In Vitro Biology. Source

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