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Moleres J.,Public University of Navarra | Santos-Lopez A.,Complutense University of Madrid | Lazaro I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Labairu J.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | And 8 more authors.
Applied and Environmental Microbiology | Year: 2015

Haemophilus parasuis, the causative agent of Glässer's disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer's disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria. © 2015, American Society for Microbiology.


Macua J.I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Santos A.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Malumbres A.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Cebolla-Cornejo J.,Polytechnic University of Valencia | And 3 more authors.
Acta Horticulturae | Year: 2015

Processing tomatoes are one of the most important crops in the agricultural and food industry of the Ebro Valley, covering at present a total surface area of 2,500 ha. The aim of this study was to analyse the agronomic behaviour of different "all flesh" cultivars of processing tomato in Navarra. The trials were conducted between 2011 and 2012 at the experimental farm run by the Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias (INTIA) in Cadreita, southern Navarra. Seven cultivars of processing tomato were tested in the field, five "all flesh" cultivars and two cultivars employed as controls. The commercial yield and mean weight of the fruit were recorded during harvesting. The fruit quality parameters measured were pH, °Brix and Hunter-scale colour (a/b). In relation to industrial quality, no significant differences were observed between the cultivars in any of the parameters analysed. Although "all flesh" cultivars are less productive than the cultivars commonly used by farmers in Navarra, they generally offer better industrial quality characteristics and contribute to satisfying current consumer demands for a wider diversity of products.


Valcarcel M.,Polytechnic University of Valencia | Leiva-Brondo M.,Polytechnic University of Valencia | Macua J.I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Lahoz I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | And 2 more authors.
Acta Horticulturae | Year: 2015

Water deficit on tomato taste-related components was studied in standard and high lycopene cultivars. The treatment was applied once the fruits were set to avoid drastic effects on yield. Despite applying considerable reductions in water doses down to 50% ETc, the contents of fructose, malic and citric acid remained unaffected. Only glucose concentration increased with lower irrigation doses in one of the locations used for the study. The cultivar effect has a major impact in tasterelated components, which might be shaded by location effects. Deficit irrigation during the last part of the growing cycle may contribute to a better water management while offering little or positive effects on the taste profile of processing tomato cultivars.


Leiva-Brondo M.,Polytechnic University of Valencia | Marti R.,Jaume I University | Macua J.I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Lahoz I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | And 2 more authors.
Acta Horticulturae | Year: 2015

The optimization of tomato taste has gained importance during the last years following consumer demands of higher quality. At the same time consumers also demand a minimum impact on the environment during cultivation. The effect of conventional and organic cultivation on the sugar and acid profile of processing cultivars has been studied in the two main growing regions in Spain. Standard and high lycopene cultivars have been used in order to study the possibility of combining organoleptic and functional quality. High differences in the acid profile (malic vs. citric) and in the sugar accumulation of the cultivars were found. In some cases, the higher values in the content of taste-related compounds coincide with previously described high lycopene contents. The cultivation system had no significant effect on malic and citric acid accumulation, but it affected sugar accumulation and the sucrose equivalents (SEq) to citric and glutamic acid ratios. On average, 7.7 and 10.4% higher contents of glucose and fructose respectively were obtained with the organic cultivation system. The SEq to citric acid and SEq to glutamic acid ratios were consequently higher with this system (8.3 and 46.3% respectively). Organic cultivation of high lycopene cultivars might be an excellent selection to combine high organoleptic and functional quality targeted to quality markets.


Lahoz I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | Leiva-Brondo M.,Polytechnic University of Valencia | Marti R.,Jaume I University | Macua J.I.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | And 2 more authors.
Scientia Horticulturae | Year: 2016

The effect of conventional integrated pest management and organic farming production systems on the agronomic performance and quality of standard and high lycopene tomato cvs. has been evaluated for two years in two of the main processing tomato producing areas of Spain (Extremadura and Navarra). As an average, the production under organic farming was on average 36% lower than in conventional integrated pest management. Organic farming tended show reduced contents of citric and glutamic acid. Although the contents in sugars were not significantly affected, the ratios sucrose equivalents to citric and glutamic acid increased. Nevertheless, a strong influence of the environment and interactions were detected and under certain conditions (e.g. Extremadura), organic farming may increase the contents in glucose and fructose. The levels of lycopene were not affected by the cultivation system, while beta-carotene contents were higher under organic farming. High lycopene cvs. 'Kalvert' and 'ISI-24424' registered the highest lycopene levels, but with 27.6 and 28.1% lower production levels compared to 'H-9036', the cv. with the best agronomic performance. 'Kalvert', with high accumulation of sugars and high ratios sucrose equivalents to citric and glutamic acid and high lycopene contents would be an ideal material for supplying quality markets. 'H-9997' with intermediated levels of lycopene accumulation proved to be a good material combining production levels and functional quality. 'CXD-277' offered the higher values in variables related with organoleptic quality with intermediate lycopene accumulation but with lower production. © 2016 Elsevier B.V.


Sanz A.,University of Zaragoza | Martin-Burriel I.,University of Zaragoza | Cons C.,University of Zaragoza | Reta M.,Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia | And 3 more authors.
Journal of Animal Breeding and Genetics | Year: 2014

The Casta Navarra lineage was one of the populations used to establish the fighting bull (FB) breed, and it has also been reproductively isolated from the others FBs. A total of 1284 individuals from two generations of 16 Casta Navarra herds were sampled to analyse their diversity, their genetic structure and the ability of 28 microsatellite markers to assign individuals to closely related populations. These animals were compared with closely related phylogenetic (FB) or geographical (Pirenaica and Monchina) populations. Hardy-Weinberg equilibrium analysis showed that 82% of the loci had a significant heterozygote deficit as a consequence of the Wahlund effect. The average proportion of genetic variation explained by farm differences was 9% by Wright's FST index. A phylogenetic tree constructed with a neighbour-joining method based on Reynolds genetic distances and a Bayesian Markov chain Monte Carlo clustering approach revealed clear differences between farm groups that generally corresponded to historical information and could unambiguously differentiate Casta Navarra cattle from the other populations. The percentage of animals correctly assigned to the Casta Navarra population was 91.78% for a q threshold of >0.9. Admixture was only detected in 4.45% (q < 0.8) of the cattle. These results are relevant for the maintenance and development of diversity and conservation in the Casta Navarra population. © 2013 Blackwell Verlag GmbH.


PubMed | Complutense University of Madrid, Autonomous University of Barcelona, Instituto Navarro Of Tecnologias E Infraestructuras Agroalimentarias Intia, Public University of Navarra and 2 more.
Type: Journal Article | Journal: Applied and environmental microbiology | Year: 2015

Haemophilus parasuis, the causative agent of Glssers disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 -lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glssers disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of -lactam resistance genes which can be transferred to pathogens or other bacteria.

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