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Byrne B.A.,University of California at Davis | Leon M.,Instituto Colombiano Agropecuario ICA | Vanegas C.,University of Los Andes, Colombia | Coral A.,Carulla S.A. Laboratorio de Calidad | And 5 more authors.
Journal of Food Protection | Year: 2015

As a step toward implementing the Colombian Integrated Program for Antimicrobial Resistance Surveillance (COIPARS), this study aimed to establish the baseline antimicrobial resistance patterns of Salmonella serovars, Escherichia coli, and Enterococcus spp. isolates in retail poultry meat from independent stores and from a main chain distributor center. MICs of the isolates were determined for antimicrobials used both in humans and animals, using an automated system. Salmonella serovars were isolated from 26% of the meat samples and E. coli from 83%, whereas Enterococcus faecalis and Enterococcus faecium were detected in 81 and 13% of the meat samples, respectively. A principal finding of concern in this study was that almost 98% of isolates tested were multidrug resistant. Ceftiofur, enrofloxacin, nalidixic acid, and tetracycline were the antimicrobials that showed the highest frequency of resistance among Salmonella and E. coli isolates. For enterococci, 61.5% of E. faecium isolates were found to be resistant to quinupristin-dalfopristin; this is significant because it is used to treat nosocomial infections when vancomycin resistance is present. Vancomycin resistance was detected in 4% of the E. faecalis isolates. The results of our study highlight the need for rapid implementation of an integrated program for surveillance of antimicrobial resistance by the Colombian authorities in order to monitor trends, raise awareness, and help promote practices to safeguard later generation antimicrobial agents. Copyright ©, International Association for Food Protection.

Introduction: Listeria monocytogenes is an intracellular, opportunistic pathogen which can cause severe infections such as meningitis, encephalitis and bacteremia. It can also cause abortions in human beings. Foods are the vehicle for infection of the host. Serotypification has discriminated 13 serotypes: 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a, 4ab, 4b, 4c, 4d, 4e, 7. 4b is the cause of the majority of cases of listeriosis in the world. Objective: The frequency of serotypes of L. monocytogenes was determined in bacteria isolated from foods in Colombia. Materials and methods: The study is descriptive and retrospective. Over a 10-year period, 2000-2009, 1, 599 isolates were examined. All were confirmed as Listeria monocytogenes and other strains of Listeria, using biochemical tests recommended by the Food and Drug Administration (USA) and API Listeria and serotyped using the Seeliger and Höhne method. Results: Of the 1, 599 isolates, 1, 424 were confirmed as L. monocytogenes. Serotypes identified were: 1/2a, 135 (9.5%); 1/2b, 154 (10.8%); 1/2c, 68 (4.8%); 3a, 4 (0.3%); 3b, 29 (2.0%); 3c, 2 (0.1%); 4a, 44 (3.1%); 4b, 820 (57.6%); 4c, 6 (0.4%); 4d- 4e, 140 (9.8%); 4e, 17 (1.2%); 7, 2 (0.1%); not susceptible of serotypification, three cases, (0.2%). Isolates came mainly from the Capital District of Bogotá, 1, 035 (73%); from Antioquia 199 (14%), from Nariño, 109 (8%); from Valle del Cauca 50 (3, 5%) and from other provinces 33 (2.3%). Conclusion: Of the analyzed isolates, 1, 424 (89%) belonged to L. monocytogenes, showing a good quality in isolation and identification. Most of these isolates belonged to serotype 4b, 820 (57.6%), a highly virulent serotype. Obligatory surveillance of this microorganism is recommended.

Munoz A.I.,Instituto Nacional Of Vigilancia Of Medicamentos Y Alimentos | Vargas M.,Instituto Nacional Of Vigilancia Of Medicamentos Y Alimentos | Otero L.,Instituto Nacional Of Vigilancia Of Medicamentos Y Alimentos | Diaz G.,Instituto Nacional Of Vigilancia Of Medicamentos Y Alimentos | Guzman V.,Instituto Nacional Of Vigilancia Of Medicamentos Y Alimentos
Biomedica | Year: 2011

Introduction: The increase in diseases transmitted by foods has become a public health problem. Listeria monocytogenes, the etiological agent of listeriosis or typical food poisoning, is considered to be among the most important food borne illnesses. High risk foods for L. monocytogenes have been recognized as foods already prepared for consumption and made available in retail outlets. Objective: The presence of L. monocytogenes was identified in prepared foods found in open markets and delicatessens in the city of Bogotá. Material and Methods: A transverse descriptive study over a 7-year period, with an analytic component, in which 600 foods were analyzed-300 from delicatessens and 300 from open market places. Reference methods were used to determine the presence or absence of L. monocytogenes in 25 grams or milliliters a food sample. Results: Of the 600 samples, 68 were positive for L. monocytogenes (11.3%). Twenty-six (38.2%) were taken from delicatessens and 42 (61.8%) from open market places. The serotype most frequently isolated was 4b, with 53 (78.0%) isolations. Fresh cheeses and matured cheeses showed greater contamination with L. monocytogenes than the other foods (p<0.001 in both cases). Conclusions: The results indicate that prepared foods are vehicles for the transmission of the Listeria bacterium. These potentially high risk foods are candidates for control and monitoring by public health authorities. Programs must be instituted to implement surveillance norms that lead to the reduction and control of this microorganism and geared toward the prevention of food borne diseases.

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