Instituto Nacional Of Saude Doutor Ricardo Jorge

Lisbon, Portugal

Instituto Nacional Of Saude Doutor Ricardo Jorge

Lisbon, Portugal
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Rodrigues A.P.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Pinto P.,Centro Hospitalar Lisbon Norte | Pinto P.,University of Lisbon | Nunes B.,Instituto Nacional Of Saude Doutor Ricardo Jorge | And 2 more authors.
Revista Portuguesa de Pneumologia | Year: 2017

Introduction: Obstructive Sleep Apnea (OSA) is characterized by recurrent episodes of apnea and hypopnea, secondary to collapse of the upper airways during sleep. OSA is frequently associated to cardiovascular complications. In Portugal, its magnitude is unknown. Methods: In 2014 a cross-sectional study was performed using the Portuguese General Practi- tioner (GP) Sentinel Network (Rede Médicos Sentinela). Participants GP reported all OSA cases diagnosed and registered in their lists of users on the 31 December 2013. Frequency of OSA has been estimated by sex and age. OSA patients were also characterized by method of diagnosis, treatment, and underlying conditions. Association between risk factors and severe OSA (odds ratio) was calculated using a logistic regression model adjusting confounding. Results: Prevalence of OSA on the population aged 25 years or more was 0.89% (95 CI: 0.80-1.00%); it was higher in males 1.47% (95 CI: 1.30-1.67%) and in those aged between 65 and 74 (2.35%). Most had severe OSA (48.4%). Hypertension (75.9%), obesity (74.2%) and diabetes mellitus (34.1%) were the most frequent comorbidities. Being a male (OR: 2.6; 95 CI: 1.2-5.8) and having obesity (OR: 4.0; 95 CI: 1.8-8.6) were associated with an increased risk of severe OSA. Conclusion: Found frequency of OSA was lower than other countries estimates, which may be explained by differences on case definition but can also suggest underdiagnosis of this condition as reported by other authors. © 2017 Sociedade Portuguesa de Pneumologia.


Coelho I.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Castanheira I.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Bordado J.M.,University of Lisbon | Donard O.,University of Pau and Pays de l'Adour | Silva J.A.L.,University of Lisbon
TrAC - Trends in Analytical Chemistry | Year: 2017

Strontium is a chemical element without biological functions in humans. In fact, only two biological roles are known, both as a biomineral constituent. However, strontium and calcium (a significant biometal) present chemical similarities; hence, the absorption of the former by living organisms is plausible. Based on the particular properties of isotopes of strontium several applications of this chemical element in biological sciences have been developed. These include its radioactive and non-radioactive isotopes in medical applications or resorting to its stable isotopes as analytical tools in isotope fingerprinting (87Sr/86Sr) to study dietary habits and migration patterns of ancient populations, food authentication and forensic sciences. This article, reviews papers published between 2012 and 2016, highlights the use of strontium isotope ratios for such biological related applications and compiles an analytical database, providing a valuable tool for future studies in the area. © 2017 Elsevier B.V.


da Costa P.J.,Instituto Nacional Of Saude Doutor Ricardo Jorge | da Costa P.J.,University of Lisbon | Menezes J.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Menezes J.,University of Lisbon | And 2 more authors.
International Journal of Biochemistry and Cell Biology | Year: 2017

Alternative pre-mRNA splicing (AS) affects gene expression as it generates proteome diversity. Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature translation-termination codons (PTCs), preventing the production of truncated proteins that could result in disease. Several studies have also implicated NMD in the regulation of steady-state levels of physiological mRNAs. In addition, it is known that several regulated AS events do not lead to generation of protein products, as they lead to transcripts that carry PTCs and thus, they are committed to NMD. Indeed, an estimated one-third of naturally occurring, alternatively spliced mRNAs is targeted for NMD, being AS coupled to NMD (AS-NMD) an efficient strategy to regulate gene expression. In this review, we will focus on how AS mechanism operates and how can be coupled to NMD to fine-tune gene expression levels. Furthermore, we will demonstrate the physiological significance of the interplay among AS and NMD in human disease, such as cancer and neurological disorders. The understanding of how AS-NMD orchestrates expression of vital genes is of utmost importance for the advance in diagnosis, prognosis and treatment of many human disorders. © 2017 Elsevier Ltd.


Valerio E.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Valerio E.,University of Porto | Campos A.,University of Porto | Osorio H.,University of Porto | Vasconcelos V.,University of Porto
Toxicon | Year: 2016

Some of the most common toxins present in freshwater, in particular microcystins (MCs), are produced by cyanobacteria. These toxins have a negative impact on human health, being associated with episodes of acute hepatotoxicity and being considered potentially carcinogenic to humans. To date the exact mechanisms of MC-induced toxicity and tumor promotion were not completely elucidated. To get new insights underlying microcystin-LR (MCLR) molecular mechanisms of toxicity we have performed the proteomic profiling using two-dimensional electrophoresis and MALDI-TOF/TOF of Saccharomyces cerevisiae cells exposed for 4 h-1 nM and 1 μM of MCLR, and compared them to the control (cells not exposed to MCLR). We identified 14 differentially expressed proteins. The identified proteins are involved in metabolism, genotoxicity, cytotoxicity and stress response. Furthermore, we evaluated the relative expression of yeast's PP1 and PP2A genes and also of genes from the Base Excision Repair (BER) DNA-repair system, and observed that three out of the five genes analyzed displayed dose-dependent responses. Overall, the different proteins and genes affected are related to oxidative stress and apoptosis, thus reinforcing that it is probably the main mechanism of MCLR toxicity transversal to several organisms, especially at lower doses. Notwithstanding these MCLR responsive proteins could be object of further studies to evaluate their suitability as biomarkers of exposure to the toxin. © 2016 Elsevier Ltd. All rights reserved.


Machado J.C.,University of Porto | Faria M.A.,University of Porto | Melo A.,University of Porto | Melo A.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Ferreira I.M.P.L.V.O.,University of Porto
Journal of Functional Foods | Year: 2017

Xanthohumol, isoxanthohumol, α and β-acids and iso-α-acids from hops exhibit beneficial properties with promising effects in cancer therapy, however information regarding their effects on colon Caco-2 cells is missing. Herein these compounds and a beer base matrix were tested for antiproliferative activity in the referred cells. Xanthohumol did not show antiproliferative activity at concentrations from 0.313 to 20 µg/mL. Isoxanthohumol was able to inhibit proliferation by 25% at 10–20 µg/mL when pure solution and increase 7–13% the activity of the matrix when spiked. α and β-acids and iso-α-acids, presented IC50 values of 16.16 ± 3.11 and 50.61 ± 13.08 µg/mL, respectively. A synergist effect with the beer matrix was noticed for α and β-acids and iso-α-acids which increased the activity by 36% and 23% at 80 and 100 µg/mL, respectively. The antiproliferative effects of the beer matrix components are thus dependent on both brewing process and hops addition. © 2017 Elsevier Ltd


Soares A.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Carrascosa C.,University of Las Palmas de Gran Canaria | Raposo A.,Ho Chi Minh City University of Technology
Food and Bioprocess Technology | Year: 2017

Broccoli belongs to the Brassicaceae family and the Brassica genus, also designated crucifers, which has been linked to reduced risk of certain diseases for their content of compounds like glucosinolates (GLS) and vitamin C. Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates, are substances that protect cells against oxidative stress, and present many other health benefits. Vitamin C also contains antioxidant properties that contribute to the beneficial effects that broccoli have for health. The present paper is an overview of the reduction of secondary plant products, such as GLS and vitamin C, by cooking. Nonetheless, these concentrations can be modified if the cell structure of the plant is disrupted, e.g., while cutting, chewing, or cooking. Myrosinase can come into contact with GLS and hydrolyze it to isothiocyanate sulforaphane or sulforaphane nitrile, depending on the environmental conditions, which produces changes in the composition and concentration of GLS. Thus, cooking induces many chemical and physical modifications in food, among which GLS and vitamin C content in broccoli can change. Vitamin C and GLS are water-soluble, which makes them more susceptible to loss during the cooking process. Despite some controversy, most reviewed studies show that conventional cooking methods (boiling, steaming, and frying) and nonconventional ones (microwaving) significantly lead to the degradation of vitamin C and GLS. Nonetheless, steaming is the conventional method that obtains the best result to better preserve these two compounds. © 2017 Springer Science+Business Media New York


D'Antuono F.,Campus Universitario Of Science Degli Alimenti | Sanches-Silva A.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Costa H.S.,Instituto Nacional Of Saude Doutor Ricardo Jorge
Nutrition Bulletin | Year: 2010

The Sustainable exploitation of bioactive components from the Black Sea Area traditional foods (BaSeFood) is a 3-year collaborative research programme, funded by the 7th Framework Programme, launched on the 1st of April 2009. The project, which is coordinated by Dr Filippo D'Antuono (University of Bologna), consists of a research consortium of 13 partners, namely Italy (two), the United Kingdom, Greece, Portugal, Serbia and six Black Sea area countries: Russian Federation, Ukraine (two), Romania, Bulgaria, Turkey and Georgia. BaSeFood will contribute scientifically by studying the bioactive compounds within traditional foods of the Black Sea area using rigorous analytical and biological assays. The vast array of characteristics of traditional foods will be considered, as well as any associated consumer-perceived benefits, related to health claims, so that they can be properly understood by the consumer and exploited by food processors to produce more healthy traditional foods. © 2010 The Authors. Journal compilation © 2010 British Nutrition Foundation.


Valente A.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Albuquerque T.G.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Sanches-Silva A.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Costa H.S.,Instituto Nacional Of Saude Doutor Ricardo Jorge
Food Research International | Year: 2011

Ascorbic acid (AA) is a water-soluble vitamin mainly present in fruits and vegetables. Food Composition Databases (FCDB) provide detailed information on nutritionally important components in foods. However, in some FCDB there is a significant lack of information on vitamin C content. The aim of this study is to produce new data for FCDB by measuring the AA content in 26 types of exotic fruits and to evaluate the nutritional value of these fruits as a source of vitamin C. In this study, the analytical method used to measure ascorbic acid content is an economic, rapid and previously validated High Performance Liquid Chromatography (HPLC) method. Ascorbic acid content per 100. g of edible portion ranged between 0.925 ± 0.018. mg for kiwano and 117 ± 1.64. mg for arbutus. For all the analyzed exotic fruits and considering a mean daily consumption of 100. g/day, twelve of them provide more than 30% of the Dietary Reference Intake. About a quarter of the analyzed exotic fruits are not found in any of the five FCDB considered in this study. The worldwide global market is expanding the production and consumption of fresh fruits and vegetables, mostly exotic, increasing the need of updating the food composition databases with high quality data. The analytical results obtained in this study are an important source of reliable data to be included in the Portuguese food composition database. © 2011 Elsevier Ltd.


Goncalves B.,University of Trás os Montes e Alto Douro | Borges O.,Delegacao Regional de Agricultura do Nordeste Transmontano | Costa H.S.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Bennett R.,University of Trás os Montes e Alto Douro | And 2 more authors.
Food Chemistry | Year: 2010

The aim of this research was to study the processing effects (roasting and boiling) on primary and secondary metabolite composition of fruits from the following chestnut (Castanea sativa Mill.) cultivars (cvs.) of three Protected Designation of Origin (PDO) areas in the Trás-os-Montes e Alto Douro region (Portugal): PDO Terra Fria (cvs. Aveleira, Boaventura, Côta, Lamela and Trigueira), PDO Padrela (cvs. Judia, Lada, Longal and Negra) and PDO Soutos da Lapa (cvs. Longal and Martaínha). The cooking processes significantly (p < 0.0001) affected primary and secondary metabolite composition of the chestnuts. Roasted chestnuts had higher protein contents, insoluble and total dietary fibre and lower fat contents whilst boiled chestnuts had lower protein, but higher fat contents. Cooking increased citric acid contents, especially in roasted chestnuts. On the other hand, raw chestnuts had higher malic acid contents than cooked chestnuts. Moreover, roasted chestnuts had significantly higher gallic acid and total phenolics contents, and boiled chestnuts had higher gallic and ellagic acids contents, when compared to raw chestnuts. The present data confirms that cooked chestnuts are a good source of organic acids and phenolics and have low fat contents, properties that are associated with positive health benefits. © 2010 Elsevier Ltd. All rights reserved.


Albuquerque T.G.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Costa H.S.,Instituto Nacional Of Saude Doutor Ricardo Jorge | Castilho M.C.,University of Coimbra | Sanches-Silva A.,Instituto Nacional Of Saude Doutor Ricardo Jorge
Trends in Food Science and Technology | Year: 2011

Partially hydrogenated vegetable oils containing trans fatty acids (TFA) have been used in the food industry, due to its low cost compared with other fats and availability to extend the shelf-life of products, as well as to impart desirable characteristics to the food.A compilation of the available information on sample preparation and analytical methods for the determination of TFA in foodstuffs was the basis of the present extensive bibliographic review. This review contributes for a better understanding of the analytical approaches adopted to determine TFA and to follow the tendency over the years to enhance the knowledge toward TFA contents found in foodstuffs. © 2011 Elsevier Ltd.

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