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Tellez R.,CINVESTAV | Rocha L.,CINVESTAV | Castillo C.,Escuela Superior de Medicina del IPN | Castillo C.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Meneses A.,CINVESTAV
Behavioural Brain Research | Year: 2010

Serotonin transporter (SERT) has been associated with drugs of abuse like d-methamphetamine (METH). METH is well known to produce effects on the monoamine systems but it is unclear how METH affects SERT and memory. Here the effects of METH and the serotonin reuptake inhibitor fluoxetine (FLX) on autoshaping and novel object recognition (NOR) were investigated. Notably, both memory tasks recruit different behavioral, neural and cognitive demand. In autoshaping task a dose-response curve for METH was determined. METH (1.0. mg/kg) impaired short-term memory (STM; lasting less of 90. min) in NOR and impaired both STM and long-term memory (LTM; lasting 24 and 48. h) in autoshaping, indicating that METH had long-lasting effects in the latter task. A comparative autoradiography study of the relationship between the binding pattern of SERT in autoshaping new untrained vs. trained treated (METH, FLX, or both) animals was made. Considering that hemispheric dominance is important for LTM, hence right vs. left hemisphere of the brain was compared. Results showed that trained animals decreased cortical SERT binding relative to untrained ones. In untrained and trained treated animals with the amnesic dose (1.0. mg/kg) of METH SERT binding in several areas including hippocampus and cortex decreased, more remarkably in the trained animals. In contrast, FLX improved memory, increased SERT binding, prevented the METH amnesic effect and re-established the SERT binding. In general, memory and amnesia seemed to make SERT more vulnerable to drugs effects. © 2010 Elsevier B.V.


Recently, a research group has suggested the existence of women ́s disorders that may be grouped together as reproductive-related disorders, characterized by their timing (related to reproductive processes), shared vulnerability, and a common pathophysiology. Symptoms of reproductive-related disorders could be different among women, but are consistent within each individual woman and always present during reproductive-life periods including pregnancy, postpartum, and the premenstrual and perimenopausal periods. An association between hormonal changes and affective disorders is suggested as a physiopathology process of reproductive-related disorders, with an abnormal adaptation to hormonal changes in vulnerable women. In this paper we describe some characteristics of premenstrual dysphoria disorder, postpartum depression, and perimenopausal depression. A proposed pathophysiology of these disorders is described and pharmacologic options for treatment are also reviewed.


Irles C.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Arias-Martinez J.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Guzman-Barcenas J.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Ortega A.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Ortega A.,National Autonomous University of Mexico
Canadian Journal of Physiology and Pharmacology | Year: 2010

Uncovering the plasma membrane distribution of tyrosine kinase Lck is crucial to understanding T lymphocyte triggering. Several studies of Lck species partitioning have given contradictory results. We decided to re-address this point by using phospho-specific antibodies to characterize active and inactive Lck partitioning in raft and non-raft membranes from primary human peripheral blood T lymphocytes. We show that most inactive Lck was localized in rafts and was associated with nearly all CD4 coreceptors and its negative regulator Csk in resting cells, while T cell receptor (TCR) engagement promoted a sustained dephosphorylation of inactive Lck. In contrast, active Lck had a more discrete distribution interacting with only a small number of CD4 coreceptors, and the kinase showed a rapid and short phosphorylation after TCR triggering. The differences in distribution and kinetics may be related to T lymphocyte signalling threshold modulation by Lck species and suggest how TCR triggering is first initiated. This study furthers our knowledge of the TCR activation model in primary human T lymphocytes.


Aguilar-Carrasco J.C.,Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes | Rodriguez-Silverio J.,National Polytechnic Institute of Mexico | Del Carmen Carrasco-Portugal M.,Instituto Nacional Of Enfermedades Respiratorias Ismael Cosio Villegas | Flores-Murrieta F.J.,National Polytechnic Institute of Mexico | Flores-Murrieta F.J.,Instituto Nacional Of Enfermedades Respiratorias Ismael Cosio Villegas
Journal of Liquid Chromatography and Related Technologies | Year: 2011

A new simple and sensitive method for determination of ketoprofen in micro-whole blood samples was developed, and its usefulness was demonstrated by the characterization of the oral pharmacokinetics of this drug in rats. Whole blood samples were added with perchloric acid to precipitate proteins, and supernatant was injected into the chromatographic system. Separation of compounds was achieved with a Symmetry C18 column eluted with a mixture of acetonitrile and 0.1M potassium dihydrogen phosphate solution pH 3.5 (48:52v/v) as the mobile phase at flow rate of 1.0mL/min. Detection was carried out by absorbance at 260nm. Under these conditions the method was linear in the range 0.1-15g/mL. In order to demonstrate the usefulness of this method, oral pharmacokinetics of ketoprofen after administration of 1, 3.2, and 10mg/kg was evaluated in rats. Blood samples were obtained at selected times during 24hr and successfully analyzed by the method described. It is concluded that the method is suitable for pharmacokinetic studies of ketoprofen using small volume of sample. Copyright © Taylor & Francis Group, LLC.


PubMed | Instituto Nacional Of Perinatologia Isidro Espinoza Of Los Reyes
Type: Journal Article | Journal: Gaceta medica de Mexico | Year: 2011

Recently, a research group has suggested the existence of womens disorders that may be grouped together as reproductive-related disorders, characterized by their timing (related to reproductive processes), shared vulnerability, and a common pathophysiology. Symptoms of reproductive-related disorders could be different among women, but are consistent within each individual woman and always present during reproductive-life periods including pregnancy, postpartum, and the premenstrual and perimenopausal periods. An association between hormonal changes and affective disorders is suggested as a physiopathology process of reproductive-related disorders, with an abnormal adaptation to hormonal changes in vulnerable women. In this paper we describe some characteristics of premenstrual dysphoria disorder, postpartum depression, and perimenopausal depression. A proposed pathophysiology of these disorders is described and pharmacologic options for treatment are also reviewed.

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