Instituto Nacional Of Perinatologia

Mexico City, Mexico

Instituto Nacional Of Perinatologia

Mexico City, Mexico
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Vidal-Cantu G.C.,CINVESTAV | Jimenez-Hernandez M.,La Salle University at Cuauhtémoc | Rocha-Gonzalez H.I.,National Polytechnic Institute of Mexico | Villalon C.M.,CINVESTAV | And 3 more authors.
European Journal of Pharmacology | Year: 2016

Sumatriptan, dihydroergotamine and methysergide inhibit 1% formalin-induced nociception by activation of peripheral 5-HT1B/1D receptors. This study set out to investigate the pharmacological profile of the antinociception produced by intrathecal and intraplantar administration of ergotamine (a 5-HT1B/1D and 5-HT5A/5B receptor agonist) and valerenic acid (a partial agonist at 5-HT5A receptors). Intraplantar injection of 1% formalin in the right hind paw resulted in spontaneous flinching behavior of the injected hindpaw of female Wistar rats. Intrathecal ergotamine (15 nmol) or valerenic acid (1 nmol) blocked in a dose dependent manner formalin-induced nociception. The antinociception by intrathecal ergotamine (15 nmol) or valerenic acid (1 nmol) was partly or completely blocked by intrathecal administration of the antagonists: (i) methiothepin (non-selective 5-HT5A/5B; 0.01-0.1 nmol); (ii) SB-699551 (selective 5-HT5A; up to 10 nmol); (iii) anti-5-HT5A antibody; (iv) SB-224289 (selective 5-HT1B; 0.1-1 nmol); or (v) BRL-15572 (selective 5-HT1D; 0.1-1 nmol). Likewise, antinociception by intraplantar ergotamine (15 nmol) and valerenic acid (10 nmol) was: (i) partially blocked by methiothepin (1 nmol), SB-699551 (10 nmol) or SB-224289 (1 nmol); and (ii) abolished by BRL-15572 (1 nmol). The above doses of antagonists (which did not affect per se the formalin-induced nociception) were high enough to completely block their respective receptors. Our results suggest that ergotamine and valerenic acid produce antinociception via 5-HT5A and 5-HT1B/1D receptors located at both spinal and peripheral sites. This provides new evidence for understanding the modulation of nociceptive pathways in inflammatory pain. © 2016 Elsevier B.V. All rights reserved.


Viteri F.E.,University of California at Berkeley | Viteri F.E.,Childrens Hospital Oakland Research Institute | Casanueva E.,Instituto Nacional Of Perinatologia | Tolentino M.C.,Instituto Nacional Of Perinatologia | And 2 more authors.
Reproductive Toxicology | Year: 2012

Universal prenatal daily supplementation with iron (60-120. mg iron) plus folic acid (0.4. mg), as recommended by INACG/WHO/UNICEF, prevents anemia where iron deficiency is prevalent but may be excessive for non-anemic women. Weekly supplementation with 120. mg iron plus various amounts of folic acid similarly prevents significant anemia. Objective: Determine, in non-anemic pregnant women, if oxidative stress is produced by recommended daily or weekly supplementation schemes. Procedure: 100 non-anemic pregnant women, 30% iron-deficient at week 20, were randomly supplemented daily followed by weekly, each for 8 weeks, or in reversed order. Results: With daily supplementation thio-barbituric-acid-reacting-substances (TBARS) increased significantly and high serum ferritin, iron, and excessively elevated hemoglobin occurred near term. During weekly supplementation significant anemia and high iron parameters were prevented, and elevated TBARS declined. Conclusion: In non-anemic pregnant women both schemes prevented significant anemia. Oxidative stress occurred only during daily supplementation periods. Weekly supplementation appears safer. © 2012 Elsevier Inc.


Gonzalez-Hernandez A.,CINVESTAV | Manrique-Maldonado G.,CINVESTAV | Lozano-Cuenca J.,CINVESTAV | Munoz-Islas E.,CINVESTAV | And 4 more authors.
European Journal of Pharmacology | Year: 2011

We have previously shown that 5-HT1B receptors inhibit prejunctionally the rat vasodepressor CGRPergic sensory outflow. Since 5-HT 1 receptors comprise 5-HT1A, 5-HT1B, 5-HT 1D and 5-HT1F functional subtypes, this study has further investigated the role of 5-HT1A, 5-HT1D and 5-HT 1F receptor subtypes in the inhibition of the above vasodepressor sensory outflow. Pithed rats were pretreated with i.v. continuous infusions of hexamethonium and methoxamine, followed by 5-HT1 receptor agonists. Then electrical spinal stimulation (T9-T12) or i.v. bolus injections of exogenous α-CGRP produced frequency-dependent or dose-dependent vasodepressor responses. The electrically-induced vasodepressor responses remained unchanged during infusions of the 5-HT1A receptor agonists 8-OH-DPAT and NN-DP-5-CT. In contrast, these responses were inhibited by the agonists sumatriptan (5-HT1A/1B/1D/1F), indorenate (5-HT 1A), PNU-142633 (5-HT1D) or LY344864 (5-HT1F), which did not affect the vasodepressor responses to exogenous CGRP (implying a prejunctional sensory-inhibition). When analysing the effects of antagonists: (i) 310 μg/kg (but not 100 μg/kg) GR127935 (5-HT1A/1B/1D/1F) abolished the inhibition to sumatriptan, indorenate, PNU-142633 or LY344864; (ii) 310 μg/kg SB224289 (5-HT1B) or BRL15572 (5-HT1D) failed to block the inhibition to sumatriptan or PNU-142633, whereas SB224289 + BRL15572 partly blocked the inhibition to sumatriptan; and (iii) 10 μg/kg WAY100635 (5-HT1A) failed to block the inhibition to indorenate. These results suggest that 5-HT1F, but not 5-HT1A or 5-HT1D, receptor subtypes inhibit the vasodepressor sensory CGRPergic outflow although, admittedly, no selective 5-HT1F receptor agonist is available yet. The pharmacological profile of these receptors resembles that shown in rat dorsal root ganglia by molecular biology techniques. © 2011 Elsevier B.V.


Fernandez-Carrocera L.A.,Neonatology | Fernandez-Carrocera L.A.,Instituto Nacional Of Perinatologia | Solis-Herrera A.,Neonatology | Cabanillas-Ayon M.,Neonatology | And 4 more authors.
Archives of Disease in Childhood: Fetal and Neonatal Edition | Year: 2013

Background: A randomised, double-blind clinical trial was undertaken in order to assess the effectiveness of probiotics in the prevention of necrotising enterocolitis (NEC) in newborns weighing <1500 g. Methods: We studied a group of 150 patients who were randomised in two groups after parental consent was obtained, to receive either a daily feeding supplementation with a multispecies probiotic (Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum, Bifidobacteruim infantis, Streptococcus thermophillus) 1 g per day plus their regular feedings or to receive their regular feedings with nothing added (control group), over the period of January 2007 through June 2010. Clinicians in care of the infants were blinded to the group assignment. Results: The primary outcome was the development of NEC. Both groups were comparable, with no differences during hospitalisation, including the type of nutrition received. Blood cultures obtained from cases that developed sepsis did not reveal lactobacillus or Bifidobacteria growth. No differences were detected in terms of NEC risk reduction (RR: 0.54, 95% CI 0.21 to 1.39) although we did observe a clear trend in the reduction of NEC frequency in the studied cases: 6 (8%) versus 12 (16%) in the control group. When the combined risk of NEC or death was calculated as a post hoc analysis, we found a significantly lower risk (RR: 0.39, 95% CI 0.17 to 0.87) for the study group. Conclusions: Probiotics may offer potential benefits for premature infants and are a promising strategy in the reduction of the risk of NEC in preterm newborns.


Perichart-Perera O.,Instituto Nacional Of Perinatologi | Balas-Nakash M.,Instituto Nacional Of Perinatologi | Munoz-Manrique C.,Instituto Nacional Of Perinatologi | Legorreta-Legorreta J.,Instituto Nacional Of Perinatologi | And 3 more authors.
Menopause | Year: 2014

Objective: This study aims to compare the effects of a lifestyle intervention using a behavioral therapy (BT) approach with the effects of a cardioprotective structured hypocaloric diet on metabolic syndrome in Mexican postmenopausal women. Methods: This study is a randomized clinical trial (2006-2009) of Mexican postmenopausal women with metabolic syndrome (Adult Treatment Panel III criteria) who were recruited from the Postmenopause Clinic of the National Institute of Perinatology in Mexico City. Women were assigned to one of two groups - group 1 (structured hypocaloric diet; n = 63): energy restriction (-300 to -500 kcal/d) emphasizing cardioprotective dietary changes; and group 2 (BT; n = 55): goal setting, problem-solving, and stimulus control to achieve cardioprotective dietary and lifestyle recommendations. Metabolic syndrome prevalence, as well as weight, waist circumference, fat mass, and fasting biochemical markers (glucose and lipid profile), were measured at baseline and at 2, 4, and 6 months after the intervention. Metabolic syndrome risk (relative risk and absolute risk reduction), mean differences between groups, and logistic regression were evaluated using Statistical Package for the Social Sciences software, version 17.0. Results: A total of 118 women were studied (mean [SD] age, 53.81 [6.43] y). No baseline differences were observed between groups. At the end of the study, a higher reduction in metabolic syndrome prevalence was observed in group 1 (-38.1%) compared with group 2 (-12.7%; relative risk, 0.237; 95% CI, 0.092-0.608; P = 0.003). The effect was maintained even when adjusted by age, hormone therapy and antihypertensive drug use. Conclusions: A cardioprotective structured hypocaloric diet is more effective than the BT approach in reducing metabolic syndrome after 6 months of intervention. Both strategies have positive effects on different individual cardiovascular risk factors. © 2014 by The North American Menopause Society.


Portillo W.,National Autonomous University of Mexico | Antonio-Cabrera E.,National Autonomous University of Mexico | Camacho F.J.,National Autonomous University of Mexico | Diaz N.F.,Instituto Nacional Of Perinatologia | Paredes R.G.,National Autonomous University of Mexico
Hormones and Behavior | Year: 2013

Non-copulating (NC) males are those animals that do not mate in spite of repeated testing with sexually receptive females. They have been observed in several species including rats and mice. The present experiment was designed to perform a detailed behavioral characterization of NC male mice. Thus, we evaluated their sexual incentive motivation for a sexually receptive female or a sexually active male, olfactory preference for volatile and non-volatile odors from females or males, and olfactory discrimination between female and male volatile odors and food related odors (milk versus vinegar). We compared the activity of the accessory olfactory system (AOS) in copulating (C) and NC males in response to estrous bedding using the induction of Fos-immunoreactivity (Fos-IR) as a measure of neuronal activation. We also determined if estradiol or dopamine treatment could induce sexual behavior in NC males. Finally, we compared the testis weight and the number of penile spines in C, NC, and gonadectomized males. In the sexual incentive motivation test C males spend significantly more time in the female incentive zone than in the male incentive zone. On the other hand, NC males spend the same amount of time in both incentive zones. In tests of olfactory preference, NC males spent less time investigating estrous odors than C males. As well, NC males discriminate urine from conspecifics but they spend less time smelling these odors than C males. In addition, no increase in Fos expression is observed in NC males when they are exposed to odors from estrous females. Our data also suggest that the deficits observed in NC males are not due to lower circulating levels of gonadal hormones, because estradiol supplementation does not induce sexual behavior in these animals, and their testis weight and the number of penile spines are normal. The results suggest that NC males are not sexually motivated by the receptive females and their odors. © 2013 Elsevier Inc.


Ramirez-Torres M.A.,Instituto Nacional Of Perinatologia
Nutrition Reviews | Year: 2013

Diabetes mellitus is the main health problem affecting Mexico's population. The mechanisms by which susceptibility to it is acquired and diabetes develops are topics of ongoing research. In order to prevent type 2 diabetes, one of the challenges is to fully understand gestational diabetes and the hormonal changes and altered carbohydrate metabolism that are associated with it during fetal development. A recent study by the Instituto Nacional de Perinatología found a 12.9% prevalence of gestational diabetes; if the current criteria suggested by the American Diabetes Association were applied, this figure would rise to almost 30%. Identifying mothers and children at high risk of developing diabetes mellitus and its comorbid conditions will help facilitate the timely implementation of preventive measures. This will be a rational use of economic resources in Mexico that will vitally benefit public health. © 2013 International Life Sciences Institute.


Diaz N.F.,Instituto Nacional Of Perinatologia | Cruz-Resendiz M.S.,Instituto Nacional Of Perinatologia | Flores-Herrera H.,Instituto Nacional Of Perinatologia | Garcia-Lopez G.,Instituto Nacional Of Perinatologia | Molina-Hernandez A.,Instituto Nacional Of Perinatologia
Reviews in the Neurosciences | Year: 2014

During early and late embryo neurodevelopment, a large number of molecules work together in a spatial and temporal manner to ensure the adequate formation of an organism. Diverse signals participate in embryo patterning and organization synchronized by time and space. Among the molecules that are expressed in a temporal and spatial manner, and that are considered essential in several developmental processes, are the microRNAs (miRNAs). In this review, we highlight some important aspects of the biogenesis and function of miRNAs as well as their participation in ectoderm commitment and their role in central nervous system (CNS) development. Instead of giving an extensive list of miRNAs involved in these processes, we only mention those miRNAs that are the most studied during the development of the CNS as well as the most likely mRNA targets for each miRNA and its protein functions. © 2014 by De Gruyter.


Bernabe-Garcia M.,Hospital Of Pediatria | Lopez-Alarcon M.,Hospital Of Pediatria | Blanco-Favela F.,Hospital Of Pediatria | Mancilla-Ramirez J.,Instituto Nacional Of Perinatologia | And 2 more authors.
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2011

The use of n-3 polyunsaturated fatty acids in surgical patients has risen by the fact that this may attenuate systemic and acute inflammatory responses secondary to surgical trauma through modulation of inflammatory mediators and cell membrane interactions. Moreover, the inclusion of n-3 fatty acids in clinical trials as part of the therapy in patients, who expect to undergo a surgical stress, suggests benefits on clinical progress. Therefore, the objective of this article is to review data from n-3 polyunsaturated fatty acid effects on biochemical parameters and on reduced length of hospitalization, number of infections, and mortality as main clinical outcomes in human surgical patients. © 2011 Elsevier Ltd.


Lopez-Valdez J.A.,Centenario Hospital Miguel Hidalgo | Estrada-Juarez H.,Instituto Nacional Of Perinatologia | Moreno-Verduzco E.R.,Instituto Nacional Of Perinatologia | Aguinaga-Rios M.,Instituto Nacional Of Perinatologia
Fetal and Pediatric Pathology | Year: 2013

Sirenomelia is the most severe malformation complex affecting the human caudal pole, although its etiology is unclear, a primary defect of blastogenesis has been proposed. Studies consider sirenomelia as the most severe form of caudal dysgenesis, VACTERL association, or axial mesodermal dysplasia, although others still support the idea of a different pathologic entity. We report the prenatal, clinical, and pathologic features of a fetus with cleft lip and palate, microtia, cardiac, renal and intestinal malformations, radial aplasia, and sirenomelia. Karyotype, chromosomal breakage studies, and SHH sequence analysis were normal. The occurrence of cephalic, midline-paramedial, and caudal malformations in the same patient imply the diagnosis of hemifacial microsomia and sirenomelia. These entities are part of the same mesodermal malformation spectrum and the clinical presentation depends on environmental and genetic interactions in embrionic development. Future clinical and genome wide studies will help to better delineate this spectrum. © Informa UK, Inc.

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