Cantu-Brito C.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran |
Arauz A.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Aburto Y.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Barinagarrementeria F.,Hospital Angeles |
And 2 more authors.
European Journal of Neurology | Year: 2011
Background and purpose: Although pregnancy and postpartum have long been associated with stroke, there is a dearth of information in Latino-American populations. The aim of this study was to describe the cerebrovascular complications occurring during pregnancy/postpartum and compare the characteristics amongst stroke types occurring in this period in Hispanic women. Patients and methods: We studied 240 women with cerebrovascular complications during pregnancy and the first 5weeks postpartum, from our stroke registry. Patients were classified into three groups: cerebral venous thrombosis (CVT), ischaemic stroke (IS), and intracerebral hemorrhage (ICH). For each group, clinical data, timing of the event, and outcome were analyzed. Results: Of the 240 women, 136 had CVT (56.7%), 64 IS (26.7%), and 40 ICH (16.6%). In 72 women (30%), the event occurred during pregnancy, in 153 (64%) during postpartum, and in 15 (6%) closely related to labor. CVT was more common in the first trimester of pregnancy and in the second and third weeks following delivery; whilst IS and ICH were seen mainly during pregnancy and the first 2weeks following delivery. Pre-eclampsia/eclampsia was more common in patients with ICH (57.5%) and IS (36%) than in those with CVT (9.6%) (P<0.001). An excellent recovery (modified Rankin Scale: 0-1) was observed amongst women with CVT (64%) and IS (50%) compared to ICH (32%), (P=0.004). Conclusions: Pre-eclampsia/eclampsia is a frequent risk factor in patients with ICH and IS, but not in CVT. Stroke types clustered different within the pregnancy-postpartum period. A good prognosis is observed in patients with CVT. Click to view the accompanying paper in this issue. © 2010 The Author(s). European Journal of Neurology © 2010 EFNS.
PubMed | Virginia Commonwealth University, Instituto Nacional Of Neurologia Y Neurocirugia Mvs, Institute Prevencion Social Asuncion, Clinica de rehabilitacion Las Araucarias and 13 more.
Type: Journal Article | Journal: NeuroRehabilitation | Year: 2016
To generate normative data on the Boston Naming Test (BNT) across 10 countries in Latin America, with country-specific adjustments for gender, age, and education, where appropriate.The sample consisted of 3,779 healthy adults who were recruited from Argentina, Bolivia, Chile, Cuba, El Salvador, Guatemala, Mexico, Paraguay, Peru, and, Puerto Rico. Each subject was administered the BNT as part of a larger neuropsychological battery. A standardized five-step statistical procedure was used to generate the norms.The final multiple linear regression models explained between 3-32% of the variance in BNT scores. Although t-tests showed significant differences between men and women for Mexico, Argentina, Chile, Cuba, Guatemala, and Bolivia on the BNT, none of the six countries had an effect size larger than 0.3. As a result, gender-adjusted norms were not generated.This is the first normative multicenter study conducted in Latin America to generate norms for the BNT; this study will have substantial repercussions for the practice of neuropsychology throughout the global region.
PubMed | Virginia Commonwealth University, Instituto Nacional Of Neurologia Y Neurocirugia Mvs, Clinica de rehabilitacion Las Araucarias, National Autonomous University of Honduras and 14 more.
Type: Journal Article | Journal: NeuroRehabilitation | Year: 2016
To generate normative data on the Stroop Test across 11 countries in Latin America, with country-specific adjustments for gender, age, and education, where appropriate.The sample consisted of 3,977 healthy adults who were recruited from Argentina, Bolivia, Chile, Cuba, El Salvador, Guatemala, Honduras, Mexico, Paraguay, Peru, and, Puerto Rico. Each subject was administered the Stroop Test, as part of a larger neuropsychological battery. A standardized five-step statistical procedure was used to generate the norms.The final multiple linear regression models explained 14-36% of the variance in Stroop Word scores, 12-41% of the variance in the Stoop Color, 14-36% of the variance in the Stroop Word-Color scores, and 4-15% of variance in Stroop Interference scores. Although t-tests showed significant differences between men and women on the Stroop test, none of the countries had an effect size larger than 0.3. As a result, gender-adjusted norms were not generated.This is the first normative multicenter study conducted in Latin America to create norms for the Stoop Test in a Spanish-Speaking sample. This study will therefore have important implications for the future of neuropsychology research and practice throughout the region.
German-Castelan L.,University of the Basque Country |
Manjarrez-Marmolejo J.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Gonzalez-Arenas A.,University of the Basque Country |
Gonzalez-Moran M.G.,Laboratorio Of Biologia Of La Reproduccion Animal |
Camacho-Arroyo I.,University of the Basque Country
BioMed Research International | Year: 2014
Progesterone (P4) promotes cell proliferation in several types of cancer, including brain tumors such as astrocytomas, the most common and aggressive primary intracerebral neoplasm in humans. In this work, we studied the effects of P4 and its intracellular receptor antagonist, RU486, on growth and infiltration of U373 cells derived from a human astrocytoma grade III, implanted in the motor cortex of adult male rats, using two treatment schemes. In the first one, fifteen days after cells implantation, rats were daily subcutaneously treated with vehicle (propylene glycol, 160 L), P 4 (1 mg), RU486 (5 mg), or P4 + RU486 (1 mg and 5 mg, resp.) for 21 days. In the second one, treatments started 8 weeks after cells implantation and lasted for 14 days. In both schemes we found that P4 significantly increased the tumor area as compared with the rest of the treatments, whereas RU486 blocked P4 effects. All rats treated with P4 showed tumor infiltration, while 28.6% and 42.9% of the animals treated with RU486 and P4 + RU486, respectively, presented it. Our data suggest that P4 promotes growth and migration of human astrocytoma cells implanted in the motor cortex of the rat through the interaction with its intracellular receptor. © 2014 Liliana Germán-Castelán et al.
Diaz A.,Autonomous University of Puebla |
Diaz A.,National Autonomous University of Mexico |
Diaz A.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Limon D.,Autonomous University of Puebla |
And 3 more authors.
Journal of Alzheimer's Disease | Year: 2012
Amyloid-β (Aβ) 25-35 is able to cause memory impairment and neurodegenerative events. Recent evidence has shown that the injection of Aβ 25-35 into the temporal cortex (TCx) of rats increases the inflammatory response; however, it is unclear how the inflammatory process could be involved in the progression of Aβ 25-35 toxicity. In this study we investigated the role of inflammation in the neuronal damage and spatial memory impairment generated by Aβ 25-35 in rat TCx using immunohistochemistry, ELISA, and a behavioral test in the radial maze. Our findings show that Aβ 25-35 -injection into the TCx induced a reactive gliosis (GFAP and CD11b-reactivity) and an increase of pro-inflammatory cytokines (IL-1β, IL-6, IL-17, and TNF-α) in the TCx and the hippocampus at 5, 15, and 30 days after injection. Thirty days after Aβ 25-35 injection, we observed that the inflammatory reaction probably contributed to increase the immunoreactivity of inducible nitric oxide synthase and nitrite levels, as well as to the loss of neurons in TCx and hippocampus. Behavioral performance showed that the neurodegeneration evoked by Aβ 25-35 delayed acquisition of learning and impaired spatial memory, because the Aβ 25-35-treated animals showed a greater number of errors during the task than the control group. Previous administration of an interleukin receptor antagonist (IL-1ra) (10 and 20 μg/μL, into TCx), an anti-inflammatory agent, suppressed the Aβ 25-35-induced inflammatory response and neurodegeneration, as well as memory dysfunction. This study suggests that the chronic inflammatory reaction could contribute to the progression of Aβ 25-35 toxicity and cause cognitive impairment. © 2012 -IOS Press and the authors.
Jacinto-Aleman L.F.,National Autonomous University of Mexico |
Hernandez-Guerrero J.C.,National Autonomous University of Mexico |
Trejo-Solis C.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Jimenez-Farfan M.D.,National Autonomous University of Mexico |
Fernandez-Presas A.M.,National Autonomous University of Mexico
Journal of Oral Pathology and Medicine | Year: 2010
Excessive fluoride ingestion has been identified as a risk factor for fluorosis and oxidative stress. The oxidative stress results from the loss of equilibrium between oxidative and antioxidative mechanisms that can produce kinase activation, mitochondrial disturbance and DNA fragmentation, resulting in apoptosis. Actually many people are exposed to no-adverted fluoride consumption in acute or chronic way. The aim of this study was to determine the effect of sodium fluoride on first molar germ in relation to its effect on antioxidative enzymes immunoexpression and apoptosis. Thirty first molar germs from 1-day-old Balb/c mice were cultured for 24 h with sodium fluoride (0 mM, 1 mM and 5 mM). Immunoexpression determination of CuZnSod, MnSod, catalase, Bax, Bid, caspase 8, caspase 9, caspase 3 and TUNEL assay were perfomed. Cellular disorganization in ameloblast and odontoblast-papilla zones was observed. CuZnSod and MnSod immunoexpression decrease in experimental groups. Caspase 8, caspase 3, Bax, Bid increase expression and more TUNEL positive cells in both experimental groups than control, suggest that apoptosis induced by fluoride is related to oxidative stress due to reduction of the enzymatic antioxidant. © 2010 John Wiley & Sons A/S.
Zavala-Tecuapetla C.,National Autonomous University of Mexico |
Zavala-Tecuapetla C.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Zavala-Tecuapetla C.,Research Center Estudios Avanzados Sede Sur |
Tapia D.,National Autonomous University of Mexico |
And 3 more authors.
Progress in Brain Research | Year: 2014
Although the pre-Bötzinger complex (preBötC) was defined as the inspiratory rhythm generator long ago, the functional-anatomical characterization of its neuronal components is still being achieved. Recent advances have identified the expression of molecular markers in the preBötC neurons that, however, are not exclusive to specific respiratory neuron subtypes and have not always been related to specific cell morphologies. Here, we evaluated the morphology and the axonal projections of electrophysiologically defined respiratory neurons in the preBötC using whole-cell recordings and intracellular biocytin labeling. We found that respiratory pacemaker neurons are larger than expiratory neurons and that inspiratory neurons are smaller than pacemaker and expiratory neurons. Other morphological features such as somata shapes or dendritic branching patterns were not found to be significantly different among the preBötC neurons sampled. We also found that both pacemaker and inspiratory nonpacemaker neurons, but not expiratory neurons, show extensive axonal projections to the contralateral preBötC and show signs of electrical coupling. Overall, our data suggest that there are morphological differences between subtypes of preBötC respiratory neurons. It will be important to take such differences in consideration since morphological differences would influence synaptic responses and action potential propagation. © 2014 Elsevier B.V.
Custodio V.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Gonzalez E.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Rubio C.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Paz C.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs
Environmental Toxicology and Pharmacology | Year: 2010
In the present study we considered the possible impairment of developmental noradrenergic maturity of the cerebellum, cerebral cortex and pons at 10-, 20- and 30-day-old rats arising from mothers subjected to 1. ppm ozone concentration during pregnancy. The noradrenaline concentration was found to be significantly reduced in the cerebellum during the study, while in the cerebral cortex and the pons it was found to be reduced at days 10 and 30 respectively as compared to controls. We concluded that prenatal exposure of 1.0. ppm ozone causes embryonic/fetal changes manifested in postnatal levels of noradrenaline concentrations in the brains of rats. © 2010 Elsevier B.V.
Mendez-Armenta M.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Nava-Ruiz C.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Fernandez-Valverde F.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Sanchez-Garcia A.,Instituto Nacional Of Neurologia Y Neurocirugia Mvs |
Rios C.,Depto. Neuroquimica
Environmental Toxicology and Pharmacology | Year: 2011
Heavy metals are ubiquitous in the environment and exposure through food and water as well as occupational sources can constitute a potential threat to human health. The mechanisms of heavy metal damage include the production of free radicals that alter mitochondrial activity, affecting cellular types like neurons and muscular fibres. We examined whether rats exposed subchronically via drinking water to low doses of heavy metals can produce alterations in muscle. Results showed that the proportion of ragged red fibres increased in muscle of rats exposed to lead and thallium, likewise slight changes in enzymatic activity of muscular fibres were also observed. © 2011 Elsevier B.V.
PubMed | Instituto Nacional Of Neurologia Y Neurocirugia Mvs
Type: | Journal: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia | Year: 2017
Epilepsy is characterized by spontaneous recurrent seizures and temporal lobe epilepsy (TLE) is the most common serious neurological example of acquired and frequent epilepsy. Oxidative stress is recognized as playing a contributing role in several neurological disorders, and most recently have been implicated in acquired epilepsies. The MTs occur in several brain regions and may serve as neuroprotective proteins against reactive oxygen species causing oxidative damage and stress. The main aim of this work was to describe the immunohistochemical localization of MT in the specimens derived from the patients affected by TLE. Histopathological examination showed NeuN, GFAP and MT immunopositive cells that were analyzed for determinate in hippocampal and parietal cortex samples. An increase in the reactive gliosis associated with increased MT expression was observed in patients with TLE.