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Alaez C.,Institute Diagnostico y Referencia Epidemiologicos | Alaez C.,Instituto Nacional Of Medicina Genomica | Flores-A H.,Institute Diagnostico y Referencia Epidemiologicos | Munguia A.,Institute Diagnostico y Referencia Epidemiologicos | Gorodezky C.,Institute Diagnostico y Referencia Epidemiologicos
Tissue Antigens | Year: 2015

The B*14:41N allele was identified in a The National Marrow Donor Program (NMDP) Hispanic donor typed by our Mexican Registry-DONORMO © 2015 John Wiley & Sons A/S. Source

Escandon-Rivera S.,National Autonomous University of Mexico | Gonzalez-Andrade M.,Instituto Nacional Of Medicina Genomica | Bye R.,National Autonomous University of Mexico | Linares E.,National Autonomous University of Mexico | And 2 more authors.
Journal of Natural Products | Year: 2012

An aqueous extract from the aerial parts of Brickellia cavanillesii attenuated postprandial hyperglycemia in diabetic mice during oral glucose and sucrose tolerance tests. Experimental type-II DM was achieved by treating mice with streptozotocin (100 mg/kg) and β-nicotinamide adenine dinucleotide (40 mg/kg). These pharmacological results demonstrated that B. cavanillesii is effective for controlling fasting and postprandial blood glucose levels in animal models. The same aqueous extract also showed potent inhibitory activity (IC50 = 0.169 vs 1.12 mg/mL for acarbose) against yeast α-glucosidase. Bioassay-guided fractionation of the active extract using the α-glucosidase inhibitory assay led to the isolation of several compounds including two chromenes [6-acetyl-5-hydroxy-2,2-dimethyl-2H-chromene (1) and 6-hydroxyacetyl-5-hydroxy-2,2-dimethyl-2H-chromene (2)], two sesquiterpene lactones [caleins B (3) and C (4)], several flavonoids [acacetin (5), genkwanin (6), isorhamnetin (7), kaempferol (8), and quercetin (9)], and 3,5-di-O-caffeoylquinic acid (10). Chromene 2 is a new chemical entity. Compounds 2, 4, 7, and 9 inhibited the activity of yeast α-glucosidase with IC50 0.42, 0.28, 0.16, and 0.53 mM, respectively, vs 1.7 mM for acarbose. Kinetic analysis revealed that compounds 4 and 7 behaved as mixed-type inhibitors with Ki values of 1.91 and 0.41 mM, respectively, while 2 was noncompetititive, with a Ki of 0.13 mM. Docking analysis predicted that these compounds, except 2, bind to the enzyme at the catalytic site. © 2012 The American Chemical Society and American Society of Pharmacognosy. Source

Grosso-Becerra M.V.,National Autonomous University of Mexico | Croda-Garcia G.,National Autonomous University of Mexico | Merino E.,National Autonomous University of Mexico | Servin-Gonzalez L.,National Autonomous University of Mexico | And 2 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2014

In a number of bacterial pathogens, the production of virulence factors is induced at 37°C; this effect is often regulated by mRNA structures formed in the 5′ untranslated region (UTR) that block translation initiation of genes at environmental temperatures. At 37°C, the RNA structures become unstable and ribosomes gain access to their binding sites in the mRNAs. Pseudomonas aeruginosa is an important opportunistic pathogen and the expression of many of its virulence-associated traits is regulated by the quorum-sensing (QS) response, but the effect of temperature on virulence-factor expression is not well-understood. The aim of this work is the characterization of the molecular mechanism involved in thermoregulation of QS-dependent virulence-factor production. We demonstrate that traits that are dependent on the QS transcriptional regulator RhlR have a higher expression at 37°C, correlating with a higher RhlR concentration as measured by Western blot. We also determined, using gene fusions and point mutations, that RhlR thermoregulation is a posttranscriptional effect dependent on an RNA thermometer of the ROSE (Repression Of heat-Shock gene Expression) family. This RNA element regulates the expression of the rhlAB operon, involved in rhamnolipid production, and of the downstream rhlR gene. We also identified a second functional thermometer in the 5′ UTR of the lasI gene. We confirmed that these RNA thermometers are the main mechanism of thermoregulation of QS-dependent gene expression in P. aeruginosa using quantitative real-time PCR. This is the first description, to our knowledge, of a ROSE element regulating the expression of virulence traits and of an RNA thermometer controlling multiple genes in an operon through a polar effect. © 2014, National Academy of Sciences. All rights reserved. Source

Tejero M.E.,Instituto Nacional Of Medicina Genomica | Tejero M.E.,Ibero-American University of Mexico
Nutrition, Metabolism and Cardiovascular Diseases | Year: 2010

Cardiovascular disease is a leading cause of death and disability in adults in Latin America. Women are more affected by these diseases than by all forms of cancer. Latin American countries have experienced rapid and uneven socioeconomic changes with a significant effect on lifestyle, demographic and health-related indicators. Differences in methodological approaches make it difficult to compare studies and health statistics across countries in the region. According to available statistics, female population in Latin American countries have lower mortality rate from coronary heart disease and higher mortality rate from cerebrovascular disease than North America. Current rates of obesity and type 2 diabetes are alarming in female in some countries. The high prevalence of risk factors forecasts an increase in cardiovascular disease for the coming decades in this region of the world. More systematic and sustained efforts for research, education, surveillance, prevention, early detection and affordable treatment are required across all Latin American countries to improve health conditions for adult population and particularly for women, who are more affected by obesity and diabetes. This article reviews the available information on cardiovascular disease and related risk factors in Latin American countries with a focus on female and to provide a brief description of selected multinational and national efforts to study and prevent this threat. © 2010 Elsevier B.V. Source

Hernandez-Lemus E.,Instituto Nacional Of Medicina Genomica
Journal of Non-Newtonian Fluid Mechanics | Year: 2010

In this paper we will outline a proposal for an irreversible thermodynamic theory of gene regulation. This theory would allow us to have a proper integration of molecular biophysical data for both, normal and abnormal gene function within the cell, hence enabling biomedical applications. The formalism is based on ideas taken from Extended Irreversible Thermodynamics. We describe how to deal with experimental data (mRNA levels) for high throughput gene expression experiments (microarrays) in order to extract information relevant for the biological characterization of gene regulatory phenomena. We then derive explicit expressions for the gene expression intensity, the chemical potentials of transcription and their associated affinities, as well as the corresponding Maxwell relations. With the aid of these, we were able to show that a change in the expression level of a single gene may affect the expression levels of the other genes and therefore of the whole genome. © 2010 Elsevier B.V. Source

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