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Jova M.C.,Instituto Nacional Of Investigaciones En Viandas Tropicales Inivit | Kosky R.G.,Santa Clara University | Cuellar E.E.,Instituto Nacional Of Investigaciones En Viandas Tropicales Inivit | Cuellar A.E.,Instituto Nacional Of Investigaciones En Viandas Tropicales Inivit
Biotechnology, Agronomy and Society and Environment | Year: 2012

Yam clone 'Belep' in temporary immersion system showed favorable results on microtubers formation. Plants cultivated in this type of culture system were the first ones to initiate microtubers formation and produced the highest microtubers number per plant than with CIS (Constant Immersion System) and SLS (Static Liquid Medium System). These microtubers showed the greatest fresh and dry weights, as well as their diameters. In temporary immersion system, we obtained the highest total microtubers number and competent microtubers as planting material. Microtubers achieved in this type of culture system were distinguished by their quality expressed in the higher dry matter content and starch concentration. In order to carry renewals of culture media and to maintain continuous culture growing for direct planting material, knowledges on preferences and ion depletion in culture medium during microtubers formation in yam cultivated in temporary immersion system were acquired. Source


Jova M.C.,Instituto Nacional Of Investigaciones En Viandas Tropicales Inivit | Kosky R.G.,Santa Clara University | Cuellar E.E.,Instituto Nacional Of Investigaciones En Viandas Tropicales Inivit
Biotechnology, Agronomy and Society and Environment | Year: 2011

The culture system type in liquid media influences yam plant growth ('Pacala Duclos' clone). In culture systems with forced renewal of internal atmosphere in culture flasks, Temporary Immersion System (TIS) and Constant Immersion System (CIS) with aeration through continuous bubbling in culture medium, higher results were obtained in morphological and physiological plant indicators in comparison with plants obtained in culture systems with passive renewal of internal atmosphere in culture flasks or Static Liquid System (SLS). In Temporary Immersion System, the best results were obtained after six weeks of culture in relation to total length (20.8 cm), axillary bud number (8.6), fresh weight (2.1 g) and dry weight (0.18 g) per plant, as well as photosynthetic pigment content (chlorophyll a, b, and total), net photosynthesis (15.3 μmol CO2.m-2.s-1), total transpiration (5.97 mmol H2O.m-2.s-1), stomatal conductance (457 μmol H2O.m-2.s-1) and leaf starch content (45.77 mg.gMF-1). Reducing sugar in culture medium with Temporary Immersion System was completely depleted, and mineral nutrients of lower contents (phosphorus, nitrogen, magnesium, calcium, iron, and manganese) in culture medium from this culture system could be related with plant growing. The results of this work could contribute to develop protocol for in vitro plant propagation of this yam clone. Source

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