Genetic variability in the microsatellite “A” at region 3’UTR of the SLC11A1gene in a herd of native goats (Capra hircus) [Variabilidad genética en el microsatélite “A” del extremo 3´UTR del gen SLC11A1 en un rebaño de cabras Criollas (Capra hircus)]
de la Rosa O.,Instituto Nacional Of Investigaciones Agricolas |
Vasquez Marin B.J.,Instituto Nacional Of Investigaciones Agricolas |
Marques A.F.,Instituto Nacional Of Investigaciones Agricolas Inia Lara |
Dickson L.,Instituto Nacional Of Investigaciones Agricolas Inia Lara
Zootecnia Tropical | Year: 2014
The SLC11A1 gene encodes a cationic transporter located in the membrane of phagolysosome of macrophages, which has active participation in the suppression of bacterial infections and has two polymorphic microsatellite (dinucleotide GT) at the end 3’UTR and some of its alleles have been associated with natural resistance to intracellular pathogens in domestic animals. In order to characterize genetic variability in microsatellite “A” at region 3’UTR of SLC11A1 gene present in a herd of native goats located in Lara state, was amplified by PCR a fragment of 233 bp from DNA isolated from 62 blood samples collected in the Center of Production of Sheep and Goats of INIA-Lara. The amplicons obtained were subjected to single strand conformational polymorphism analysis (SSCP) and samples were grouped according to the identity of its SSCP pattern; thus six analysis groups formed, representative samples of each group were used to determine the number of repetitions GT in the microsatellite “A”, by DNA sequencing. Genotypic frequencies were estimated on the basis of the nucleotide sequence corresponding to each analysis group. (GT12/GT17=0.016; GT12/GT13=0.016; GT13/GT13=0.016; GT13/GT15=0.290; GT13/GT16=0.483; GT16/GT18=0.177) and the allelic frequencies obtained were GT12=0.0161; GT13=0.4113; GT15=0.1452; GT16=0.3306; GT17=0.0081 and GT18=0.0887. Polymorphic information coefficient was 0.6390, the observed heterozygosity was 98.39% and the expected heterozygosity was 69.79%. The sampled herd is not in genetic equilibrium (P<0.05). © 2014, Instituto Nacional de Investigaciones Agricolas (INIA). All rights reserved.
Technical and economical records in the capra hircus-aloe vera production systems in the community of cauderales, Urdaneta municipality, Lara state, Venezuela [registros técnico-económicos en sistemas de producción caprino-sábila (capra hircus-aloe vera) en la comunidad de Cauderales, estado Lara]
Delgado A.,Instituto Nacional Of Investigaciones Agricolas Inia Lara |
Armas W.,Instituto Nacional Of Investigaciones Agricolas Inia Lara |
D'Aubeterre R.,Instituto Nacional Of Investigaciones Agricolas Inia Lara |
Jimenez M.,Instituto Nacional Of Investigaciones Agricolas Inia Lara |
Marchan V.,Instituto Nacional Of Investigaciones Agricolas Inia Lara
Zootecnia Tropical | Year: 2012
This research was developed in the semiarid region of Cauderales in Lara state, Venezuela. Records of 15 farms belonging to the Production Innovation Socialistic Red (PISR) of goat-Aloe were evaluated. Surveys with open and closed questions and interviews were conducted. The data collected was analyzed trough the frequency analysis and correlation. The results indicated that 64% of the farmers are older than 40 years, 44% had elementary school level and 52 had more than 20 years of work experience in the field, 20% are dedicated to goat production and 27% to aloe cropping and 53% to both activities. On the other hand, 80% had used production records and only 12% had received instructions in those matters. With regard to the kind of record used, 75% of the farms took information of the births, 50% about health problems, 34% recorded the incomes and outcomes, 20% kept records of the machinery and equipments, 13% had records of the surroundings such as kind of soils, trees and water supplies and 100% had documents of land properties. Same wise, 26% of the farmers considered that the main reason to keep records is for decision taking. It can be concluded that the majority of the farmers had used some kind of record in their farms, with a high tendency of using technical records, there was no evident correlation within variables such as age of the farmers, instruction level, kind of economical activities, participation in formal organizations and previous courses about record with regard to the production records.