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Peredo E.L.,University of Oviedo | Revilla M.A.,University of Oviedo | Reed B.M.,U.S. Department of Agriculture | Javornik B.,University of Ljubljana | And 3 more authors.
Genetic Resources and Crop Evolution | Year: 2010

Microsatellite variation at the nuclear and chloroplast genomes was evaluated for wild European and wild American hops, in order to assess the genetic diversity and origin of cultivated hops. Seven nuclear loci and 32 chloroplast loci were used in the analysis of 182 hop accessions including wild European (68), wild American (48), and cultivars (66). A total of 116 alleles were identified using 7 nuclear microsatellites showing different averages of polymorphism and distribution in the wild American and European accessions and cultivars. Two main groups were established as revealed by several statistical analyses; one including European wild accessions and cultivars and a second group consisting of American wild accessions. Three polymorphic chloroplast microsatellite loci were detected, six alleles were scored which defined a total of five haplotypes that were exclusive or presented different distribution between American and European wild accessions. A major influence of the wild European haplotypes was detected among hop cultivars. To the best of our knowledge, this is the first work reporting the use of chloroplast microsatellites in hops. © 2009 Springer Science+Business Media B.V.

Peredo E.L.,University of Oviedo | Cires E.,University of Oviedo | Fernandez Prieto J.A.,University of Oviedo | Revilla M.A.,University of Oviedo | Arroyo-Garcia R.,Instituto Nacional Of Investigacion Agraria Y Alimentaria
Acta Horticulturae | Year: 2011

In vitro establishment and micropropagation are basic techniques used as initial step in many of the plant tissue protocols such as cryopreservation. Severe physiological alterations such as changes in leaf structure, water relations and photosynthesis systems are usually associated with the in vitro establishment. In this report, the epigenetic alterations in hops caused by in vitro establishment and micropropagation are assessed using MSAP (methylation-sensitive amplified polymorphism). A 56.3% of the detected loci were monomorphic when the in vitro plants were compared to their donor field plant. Nearly 28.7% of the total loci presented the same alterations of the band profile in all analyzed in vitro plants when compared to the field donor plant. However, when the comparison was performed only among in the micropropagated plants 80.7% of the loci were monomorphic.

Santin-Montanya M.I.,Instituto Nacional Of Investigacion Agraria Y Alimentaria | Jimenez J.,Tragsa | Ocana L.,Tragsa | Sanchez F.J.,Direccion General del Agua
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes | Year: 2013

Chlorophyll fluorescence analysis (CFA) has been successfully used to rapidly determine the responses of different plants to herbicides. It has not, however, been used to test the effect of these products on invasive riparian species. This paper reports the use of CFA to determine photosynthetic activity in Arundo donax, an invasive reed causing serious problems in Mediterranean riparian habitats, in response to systemic herbicide application following cutting. Growth was measured in terms of new sprout relative height and sprout and rhizome relative biomass. CFA showed glyphosate, from half the on-label dose of 5 L ai.ha-1upwards, to have a significant effect (100% reduction) on photosynthetic activity at 21 days after treatment (DAT), while profoxydim, from half the on-label dose of 0.375 L ai.ha-1upwards, caused a 70% reduction soon after application, although these plants later recovered. Azimsulfuron, cyhalofop-butyl and penoxsulam had no significant effect on photosynthetic activity at any dose. At 60 DAT, glyphosate (from half the on-label dose of 5 L ai.ha-1upwards) was associated with a 90% reduction in sprout height, while profoxydim (from half the on-label dose of 0.375 L ai.ha-1upwards) caused a 50% reduction. No dose (0-2x the on-label dose) of azimsulfuron, penoxsulam or cyhalofop-butyl was associated with any significant growth reduction at 60 DAT. The results show that CFA can be used to successfully measure the response of these invasive plants to herbicides, and that glyphosate, and possibly profoxydim, might be used to control Arundo donax after initial cutting. © 2013 Copyright Taylor and Francis Group, LLC.

Qi Z.,Yancheng Institute of Technology | Qi Z.,University of Aberdeen | Zhang Q.,Yancheng Institute of Technology | Holland J.W.,University of Aberdeen | And 4 more authors.
Fisheries Science | Year: 2016

Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses. © 2016 Japanese Society of Fisheries Science

Warimwe G.M.,University of Oxford | Lorenzo G.,Instituto Nacional Of Investigacion Agraria Y Alimentaria | Lopez-Gil E.,Instituto Nacional Of Investigacion Agraria Y Alimentaria | Reyes-Sandoval A.,University of Oxford | And 11 more authors.
Virology Journal | Year: 2013

Background: Rift Valley Fever (RVF) is a viral zoonosis that historically affects livestock production and human health in sub-Saharan Africa, though epizootics have also occurred in the Arabian Peninsula. Whilst an effective live-attenuated vaccine is available for livestock, there is currently no licensed human RVF vaccine. Replication-deficient chimpanzee adenovirus (ChAd) vectors are an ideal platform for development of a human RVF vaccine, given the low prevalence of neutralizing antibodies against them in the human population, and their excellent safety and immunogenicity profile in human clinical trials of vaccines against a wide range of pathogens. Methods. Here, in BALB/c mice, we evaluated the immunogenicity and efficacy of a replication-deficient chimpanzee adenovirus vector, ChAdOx1, encoding the RVF virus envelope glycoproteins, Gn and Gc, which are targets of virus neutralizing antibodies. The ChAdOx1-GnGc vaccine was assessed in comparison to a replication-deficient human adenovirus type 5 vector encoding Gn and Gc (HAdV5-GnGc), a strategy previously shown to confer protective immunity against RVF in mice. Results: A single immunization with either of the vaccines conferred protection against RVF virus challenge eight weeks post-immunization. Both vaccines elicited RVF virus neutralizing antibody and a robust CD8§ssup§+§esup§ T cell response. Conclusions: Together the results support further development of RVF vaccines based on replication-deficient adenovirus vectors, with ChAdOx1-GnGc being a potential candidate for use in future human clinical trials. © 2013 Warimwe et al.; licensee BioMed Central Ltd.

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