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Soto J.,Centro Internacional Of La Papa | Medina T.,Instituto Nacional Of Investigacion Agraria Inia | Aquino Y.,Instituto Nacional Of Investigacion Agraria Inia | Estrada R.,National Major San Marcos University
Revista Peruana de Biologia | Year: 2014

This paper analyzes the genetic diversity of 79 accessions of native potato varieties (Solanum spp.) using 18 microsatellite markers. A random sample from Ayacucho, Cajamarca, Cusco, Huancavelica and Puno from "chacras" of farmers who collaborated with the "In situ conservation of native crops and wild relatives" were used. 17 markers amplified one single polymorphic locus, the mean number of alleles per locus was 8.79. The mean similarity was 0.62 and clustering indexes varied between 0.41 and 0.98. 19 loci showed a total of 166 alleles. Cuzco had the highest number of alleles (130 alleles). Of the 166 characterized alleles, 72 alleles (43.37%) were common or shared with 5 sampling sites. Puno had the highest number of exclusive alleles (8 alleles). The 42 varieties of S. tuberosum subsp. andigena showed a mean diversity of 0.74 and 18 varieties of S. x chaucha an average diversity of 0.70. Polymorphism (PIC = 0.55 to 0.85) and genetic diversity indices show that microsatellites evaluated can identify high levels of genetic diversity, but also are not sufficient to discriminate differentiated by origin or species groups. Our analyzes indicate a high genetic diversity and are consistent with inventories and morphological characterizations performed in situ, we can also conclude that there would be a common pool of genes would be found widely distributed among the regions studied. © Los autores. Source

Calvo-Pinilla E.,Pirbright Institute | Castillo-Olivares J.,Pirbright Institute | Jabbar T.,Pirbright Institute | Ortego J.,Instituto Nacional Of Investigacion Agraria Inia | And 2 more authors.
Virus Research | Year: 2014

Bluetongue (BT) is a hemorrhagic disease of ruminants caused by bluetongue virus (BTV), the prototype member of the genus Orbivirus within the family Reoviridae and is transmitted via biting midges of the genus Culicoides. BTV can be found on all continents except Antarctica, and up to 26 immunologically distinct BTV serotypes have been identified. Live attenuated and inactivated BTV vaccines have been used over the years with different degrees of success. The multiple outbreaks of BTV in Mediterranean Europe in the last two decades and the incursion of BTV-8 in Northern Europe in 2008 has re-stimulated the interest to develop improved vaccination strategies against BTV. In particular, safer, cross-reactive, more efficacious vaccines with differential diagnostic capability have been pursued by multiple BTV research groups and vaccine manufacturers. A wide variety of recombinant BTV vaccine prototypes have been investigated, ranging from baculovirus-expressed sub-unit vaccines to the use of live viral vectors. This article gives a brief overview of all these modern approaches to develop vaccines against BTV including some recent unpublished data. © 2013 The Authors. Source

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