Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine

Ituzaingó, Argentina

Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine

Ituzaingó, Argentina

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Gomez D.,Bacteriologa Clinica e Industrial | Gomez D.,Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine | Lavayen S.,Licenciada en Quimica | Lavayen S.,Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine | And 4 more authors.
Acta Bioquimica Clinica Latinoamericana | Year: 2011

The kitchen and bathroom are the places in the home most susceptible to microbial contamination. These germs are deposited on surfaces to form biofilms and may constitute grounds for potential disease sources. The aim of this study was to detect the presence and concentration of microbial load in the elements and utensils of the most critical housings in the city of Mar del Plata. Four hundred and seventy-eight samples of items and utensils collected from kitchens and bathrooms from 36 homes of families of similar socioeconomic characteristics to the cases of hemolytic uremic syndrome occurred in the same city. Fifty-two per cent of kitchens exceeded the parameters established for the study and >100UFC/sample of mesophilic aerobes, >10UFC/sample of Enterobacteriaceae and Escherichia coli and Staphylococcus aureus were detected. Thirty-seven per cent of baths exceeded the established criteria. The elements that are beyond these limits are usually found wet, becoming potential sources thereof. Recommendations were made to ensure proper hygiene in the most critical places. It is extremely important to work in health education at the household level of the community taking into account the recommendations that must be followed when preparing food.


Gomez D.,Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine | Chinen I.,Instituto Nacional Of Enfermedades Infecciosas Inei | Zotta C.M.,Instituto Nacional Of Epidemiologia Dr Juan H Jara Ine | Carbonari C.,Instituto Nacional Of Enfermedades Infecciosas Inei | And 6 more authors.
Acta Bioquimica Clinica Latinoamericana | Year: 2010

More than 500 new cases of hemolytic uremic syndrome (HUS) are annually reported in Argentina. The aim of this work was to carry out epidemiological studies on cases of HUS and their household contacts that were isolated from STEC O145 strains: NM belonging to the same cluster. In order to detect STEC, Multiplex PCR was performed to amplify Shiga toxin 1 and 2 genes and other virulence markers like eae and ehxA. STEC was sub-typified by means of separation by pulsed field electrophoresis (PFGE-XbaI). Between January and February 2006, STEC O145:NM strains were identified in three cases of HUS and one household contact. Genotypically, they were characterized as producing stx2, eae+ and ehxA +. All strains showed the same pattern by PFGE-XbaI (AREXSX01.0207) and BlnI-PFGE (AREXSA26.0018). These strains belonged to the same cluster, scattered in different areas of the city of Mar del Plata. Data from epidemiological research were not enough to establish a link between the cases. However, the possibility of occurrence of a diffuse outbreak. is not ruled out The importance of a laboratory surveillance system in real time by PFGE is stressed, as a warning mechanism that serves to strengthen the results with epidemiologic data.

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