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Amaya J.C.,University of La Rioja | Moreno N.,University Institute of Health Sciences | Salmaso N.,Hospital Enrique Vera Barros | Bazan E.,Hospital Enrique Vera Barros | And 3 more authors.
Revista Argentina de Microbiologia | Year: 2016

This work was conducted in the province of La Rioja, located in northwestern Argentina. The aim of this study was to estimate the percentage of dog feces showing the presence of antigens of Echinococcus sp. in different regions of the province. A total of 269 samples of dried canine stool were taken, which were analyzed by the copro-ELISA technique. The most affected area was zone IV, which had 30.5 % of positive samples. Zone I corresponding to the Capital Department of the province had 12 % of positivity. In other areas, the percentages ranged between 11.4 % and 14.8 %. This is the first study in the province of La Rioja on the existenceof this disease in dogs. The lack of control strategies has allowed the spread of echinococcosis. © 2015 Asociación Argentina de Microbiología.

Quiroga M.P.,CONICET | Arduino S.M.,Institute Educacion Medica e Investigaciones Clinicas Dr Norberto Quirno CEMIC | Arduino S.M.,University of Québec | Arduino S.M.,Laval University | And 6 more authors.
Infection, Genetics and Evolution | Year: 2013

The emergence of extended-spectrum β-lactamases and plasmid-mediated resistance to quinolones has been previously found to be associated with the dissemination of complex class 1 integrons in Argentina. In this study, we analyzed their distribution through time and evaluated the functionality of the Orf513 protein, which is the putative recombinase of the ISCR1 mobile element. We investigated the presence of the orf513, blaCTX-M-2, dfrA3b, qnrB10 and blaDHA-1 genes by PCR and DNA sequencing as well as their linkage to class 1 integrons in 451 non-epidemiologically related nosocomial strains resistant to at least one expanded-spectrum cephalosporin and to one aminoglycoside, isolated between 1989 and 2010 from 7 hospitals from Buenos Aires City. The epidemiology of complex class 1 integrons was found to be notably different among fermenting (94/171) and non-fermenting clinical bacilli isolates (1/280). The ISCR1::qnrB10 positive isolates were found since 1993, confirming its presence in clinical isolates more than a decade before its first description. As expected, In35::ISCR1::blaCTX-M-2 was the most common complex class 1 integron among Enterobacteriaceae isolates, particularly in Proteus mirabilis. Experimental analysis corroborated the activity of the Orf513 protein, which was found to bind specific DNA sequences containing the previously suggested oriIS region. These findings showed the high dispersion and maintenance of complex class 1 integrons across time in our nosocomial isolates. The contribution of the ISCR1 mobile element to multidrug resistant phenotypes is significant due to its sustained association to class 1 integrons. © 2013 Elsevier Ltd.

Santos P.E.,Hospital Nacional Of Pediatria Dr J P Garrahan | Cordoba S.,Instituto Nacional Of Enfermedades Infecciosas Inei Anlis | Rodero L.L.,Instituto Nacional Of Enfermedades Infecciosas Inei Anlis | Carrillo-Munoz A.J.,ACIAM | Lopardo H.A.,Hospital Nacional Of Pediatria Dr J P Garrahan
Revista Iberoamericana de Micologia | Year: 2010

Tinea capitis is an infection caused by dermatophytes of the genera Microsporum and Trichophyton, and constitutes a major health problem in Argentina. The aim of the present study was to find out the incidence of those etiological agents and the therapeutic response in patients attending a High-Complexity Paediatric Hospital within a two-year period. A total of 98 tinea capitis were diagnosed, 13 of which were Celsus kerion. Microsporum canis was isolated in 61.28%. The range of values for minimum inhibitory concentrations were >32, 0,06-4; <0,015-2; <0,015-0.25; 0.13-8; 0.06-128. γg/mL for fluconazole itraconazole, voriconazole, terbinafine, ketoconazole and griseofulvin, respectively. © 2009 Revista Iberoamericana de Micología.

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