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Reynaldi F.J.,CONICET | Reynaldi F.J.,National University of La Plata | Giacoboni G.,National University of La Plata | Cordoba S.B.,National University of La Plata | And 4 more authors.
Medical Mycology Case Reports | Year: 2017

A 2-year-old female Border collie was examined for dermatitis with a partial alopecic zone around her left front member. Six months later the lesion became swollen, alopecic with ulcerated areas. Microscopy analysis of samples showed numerous non-septate, branching, thin-walled and irregular shaped hyphal elements. Fungal cultures and molecular studies identified Saksenaea vasiformis. Treatments with griseofulvin, itraconazole and surgical debridement were used, however, fourteen months later the dog was euthanatized because of the unfavorable clinical outcome. © 2017


PubMed | Public Health England, Public Health Agency of Canada, Institute of Health Carlos III, South African National Institute for Communicable Diseases and 5 more.
Type: Journal Article | Journal: The Journal of infection | Year: 2015

Neisseria meningitidis is a leading cause of meningitis and septicaemia. The hyperinvasive ST-11 clonal complex (cc11) caused serogroup C (MenC) outbreaks in the US military in the 1960s and UK universities in the 1990s, a global Hajj-associated serogroup W (MenW) outbreak in 2000-2001, and subsequent MenW epidemics in sub-Saharan Africa. More recently, endemic MenW disease has expanded in South Africa, South America and the UK, and MenC cases have been reported among European and North American men who have sex with men (MSM). Routine typing schemes poorly resolve cc11 so we established the population structure at genomic resolution.Representatives of these episodes and other geo-temporally diverse cc11 meningococci (n=750) were compared across 1546 core genes and visualised on phylogenetic networks.MenW isolates were confined to a distal portion of one of two main lineages with MenB and MenC isolates interspersed elsewhere. An expanding South American/UK MenW strain was distinct from the Hajj outbreak strain and a closely related endemic South African strain. Recent MenC isolates from MSM in France and the UK were closely related but distinct.High resolution genomic multilocus sequence typing is necessary to resolve and monitor the spread of diverse cc11 lineages globally.


Silveyra I.M.,Hospital Gobernador Centeno | Pereyra A.M.,Hospital Gobernador Centeno | Alvarez M.G.,Hospital Gobernador Centeno | Villagran M.D.,Hospital Gobernador Centeno | And 5 more authors.
Revista Argentina de Microbiologia | Year: 2015

Enteropathogenic Escherichia coli (EPEC) is a major causative agent of acute diarrhea in children in developing countries. This pathotype is divided into typical EPEC (tEPEC) and atypical EPEC (aEPEC), based on the presence of the bfp virulence factor associated with adhesion, encoded in the pEAF plasmid. In the present study, the isolation of aEPEC O157:H16from a bloody diarrhea case in a child and his household contacts (mother, father and sister)is described. The strain was characterized as E. coli O157:H16 eae-ε-positive, sorbitol fer-menter with β-glucuronidase activity, susceptible to all antimicrobials tested, and negativefor virulence factors stx1, stx2, ehxA and bfp. XbaI-PFGE performed on all isolates showedthe AREXHX01.1040 macrorestriction pattern, with 100 % similarity. These results highlight theimportance of epidemiological surveillance of E. coli O157-associated diarrhea cases identifiedin children and their family contacts, as well as the incorporation of molecular techniques thatallow the detection of the different E. coli pathotypes. © 2015 Asociación Argentina de Microbiología. Published by Elsevier España, S.L.U.


PubMed | Hospital Gobernador Centeno and Instituto Nacional Of Enfermedades Infecciosas Anlis < >
Type: Case Reports | Journal: Revista Argentina de microbiologia | Year: 2016

Enteropathogenic Escherichia coli (EPEC) is a major causative agent of acute diarrhea in children in developing countries. This pathotype is divided into typical EPEC (tEPEC) and atypical EPEC (aEPEC), based on the presence of the bfp virulence factor associated with adhesion, encoded in the pEAF plasmid. In the present study, the isolation of aEPEC O157:H16 from a bloody diarrhea case in a child and his household contacts (mother, father and sister) is described. The strain was characterized as E. coli O157:H16 eae--positive, sorbitol fermenter with -glucuronidase activity, susceptible to all antimicrobials tested, and negative for virulence factors stx1, stx2, ehxA and bfp. XbaI-PFGE performed on all isolates showed the AREXHX01.1040 macrorestriction pattern, with 100% similarity. These results highlight the importance of epidemiological surveillance of E. coli O157-associated diarrhea cases identified in children and their family contacts, as well as the incorporation of molecular techniques that allow the detection of the different E. coli pathotypes.


Masana M.O.,Instituto Nacional de Tecnologia Agropecuaria | D'Astek B.A.,Instituto Nacional Of Enfermedades Infecciosas Anlis | Palladino P.M.,Instituto Nacional de Tecnologia Agropecuaria | Galli L.,Instituto Nacional Of Enfermedades Infecciosas Anlis | And 8 more authors.
Journal of Food Protection | Year: 2011

The non-O157 Shiga toxin-producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%) of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. XbaI pulsed-field gel electrophoresis pattern analysis showed 234 different patterns, with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013 were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated with non- O157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease. Copyright © International Association for Food Protection.


Loscha L.S.,Northeast National University | Vazqueza M.L.G.,Northeast National University | Rivasc M.,Instituto Nacional Of Enfermedades Infecciosas Anlis < > | Merinoa L.A.,Northeast National University
Revista Argentina de Microbiologia | Year: 2015

Groundwater is an important source of drinking water for many communities in Nort-hern Argentina; particularly, in the province of Chaco, where about 14 % of households usethis natural resource. Enteroaggregative Escherichia coli is an emerging pathogen whose globalimportance in public health has increased in recent years. Despite the significant risk of diseaselinked to contaminated water exposure, the prevalence of E. coli pathotypes in aquatic envi-ronments is still not so well defined. The aim of the present study was to detect the presenceof typical enteroaggregative E. coli through the recognition of its virulence factors aap, AAprobe and aggR by molecular techniques. A total of 93 water samples from different small com-munities of Chaco were analyzed. E. coli was identified in 36 (38.7 %) of the tested samples.Six strains isolated from different samples harbored the studied genes. Of these 6 isolates,3 carried the aap gene, 2 the AA probe and the last one the combination of aap/aggR genes.The prevalence of E. coli isolates harboring enteroaggregative virulence genes in groundwatersources was 6.4 %. This work represents the first contribution to the study of the presence anddistribution of virulence genes of EAEC in groundwater sources in this region of Argentina. © 2015 Asociación Argentina de Microbiología.


PubMed | Northeast National University and Instituto Nacional Of Enfermedades Infecciosas Anlis < >
Type: Journal Article | Journal: Revista Argentina de microbiologia | Year: 2015

Groundwater is an important source of drinking water for many communities in Northern Argentina; particularly, in the province of Chaco, where about 14% of households use this natural resource. Enteroaggregative Escherichia coli is an emerging pathogen whose global importance in public health has increased in recent years. Despite the significant risk of disease linked to contaminated water exposure, the prevalence of E. coli pathotypes in aquatic environments is still not so well defined. The aim of the present study was to detect the presence of typical enteroaggregative E. coli through the recognition of its virulence factors aap, AA probe and aggR by molecular techniques. A total of 93 water samples from different small communities of Chaco were analyzed. E. coli was identified in 36 (38.7%) of the tested samples. Six strains isolated from different samples harbored the studied genes. Of these 6 isolates, 3 carried the aap gene, 2 the AA probe and the last one the combination of aap/aggR genes. The prevalence of E. coli isolates harboring enteroaggregative virulence genes in groundwater sources was 6.4%. This work represents the first contribution to the study of the presence and distribution of virulence genes of EAEC in groundwater sources in this region of Argentina.


Pando M.A.,University of Buenos Aires | Pando M.A.,CONICET | Balan I.C.,Columbia University | Marone R.,Nexo Asociacion Civil | And 11 more authors.
PLoS ONE | Year: 2012

Background: The aim of this study was to estimate the prevalence of HIV and other STIs, among MSM from Buenos Aires (2007-2009). Methods: Responding Driven Sampling was used for recruitment of MSM. Participants completed a structured web-based survey and provided biological samples. Results: A total of 496 MSM were studied for HIV, HBV, HCV, and T pallidum infections. Chlamydia and HPV diagnoses were only performed in 98 and 109 participants, respectively. Prevalence of HIV was 17.3%, HBV 22.9%, HCV 7.5%, T pallidum 20.5%, HPV 83.5%, and C trachomatis 1.7%. In the year prior to the evaluation, 71% of the participants had had sex with men and/or trans and women (MMW) while 29% had not had sex with women (MM). Comparing MM to MMW, prevalence of HIV (30.7% vs. 11.9%, p<0.001), HBV (36.4% vs. 17.8%, p<0.001), T pallidum (32.1% vs. 15.7%, p<0.001), and HPV (88.3% vs. 70.4%, p = 0.039) were significantly higher among MM, whereas no significant differences were found for HCV and C trachomatis. The MM group had also significantly higher HIV incidence (5.60 vs. 4.28 per 100 persons-year, p = 0.032). HPV genotypes 16, 6, and 11 were the most frequently found; 40.7% of the MSM had more than one genotype and one high risk genotype was detected in 43.6% of participants. Conclusions: Both MM and MMW are at high risk of infection for HIV and other STIs. Rates of HIV, HBV, T pallidum and HPV infections are higher in the MM group. © 2012 Pando et al.

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