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Medeiros R.J.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Santos L.M.G.D.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Goncalves J.M.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Jacob S.D.C.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz
Toxicology Reports | Year: 2014

The present study estimated the human daily and weekly intake of inorganic elements due to consumption of fish in Rio de Janeiro state and the associated potential health risks posed by some toxic elements. All samples analyzed had values of Cd and Pb below the Maximum Tolerable Limits of 3.0mgkg-1 for Pb and 1.0mgkg-1 for Cd; only Mugil cephalus, Cynoscion leiarchus and Caranx crysos had As concentrations below 1mgkg-1, maximum limit established by Brazilian legislation. The higher values of Cd and Pb correspond to 0.22% of PTWI and the higher value of As corresponds to 8.6% of PTWI. None of the studied species showed values higher than PTWI. The higher values of Cu EDI found in Pomatomus numida correspond to 33.3% of RDA; Fe in Salmo salar and Genypterus brasiliensis corresponds to 4.3% of EDI; Mn in Sardinella brasiliensis corresponds to 7.4% of EDI; Zn in S. salar corresponds to 13.2% of EDI and Se in S. salar corresponds to 20.6% of EDI. Some species can be a good source of inorganic elements. For risk assessment, it is important to assess specific eating habits of each region to avoid underestimating the data. © 2014 The Authors.


Welz B.,Federal University of Santa Catarina | Welz B.,Federal University of Bahia | Santos L.M.G.d.,Federal University of Santa Catarina | Santos L.M.G.d.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | And 7 more authors.
Spectrochimica Acta - Part B Atomic Spectroscopy | Year: 2010

The simultaneous determination of cadmium and iron in plant and soil samples has been investigated using high-resolution continuum source graphite furnace atomic absorption spectrometry. The primary cadmium resonance line at 228.802 nm and an adjacent secondary iron line at 228.726 nm, which is within the spectral interval covered by the charge-coupled device (CCD) array detector, have been used for the investigations. Due to the very high iron content in most of the soil samples the possibility has been investigated to reduce the sensitivity and extend the working range by using side pixels for measurement at the line wings instead of the line core. It has been found that the calibration curves measured at all the analytically useful pixels of this line consisted of two linear parts with distinctly different slopes. This effect has been independent of the positioning of the wavelength, i.e., if the Cd line or the Fe line was in the center of the CCD array. The most likely explanation for this unusual behavior is a significant difference between the instrument width ΔλInstr and the absorption line width ΔλAbs, which is quite pronounced in the case of Fe. Using both parts of the calibration curves and simultaneous measurement at the line center and at the wings made it possible to extend the working range for the iron determination to more than three orders of magnitude. © 2010 Elsevier B.V. All rights reserved.


Dias M.T.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Bricio S.M.L.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Almeida D.O.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Oliveira L.A.T.,Federal University of Fluminense | And 2 more authors.
Ciencia e Tecnologia de Alimentos | Year: 2012

Foodborne disease caused by microorganisms is a problem of public health. Minas soft cheese is a national product manufactured using simple technology; it has high level of acceptance in the country making its production an important economic activity. Many microorganisms may be present in foods including the bacterium Escherichia coli (E. coli). Overall, E. coli is a harmless commensal bacterium; however, some strains may have a pathogenic potential. Several outbreaks of foodborne diseases associated with consumption of contaminated cheese have been reported, and the presence of pathogenic strains of E. coli has increased. The objective of this study was to isolate, evaluate the antimicrobial susceptibility, and characterize, by Multiplex PCR, the pathogenic E. coli strains isolated from Minas cheese commercialized in Rio de Janeiro. Thirty samples were analyzed and five strains of E. coli (EPEC) were identified. The assessment of antimicrobial susceptibility revealed 40% of the isolates resistant to ampicillin and 40% with intermediate resistance to ampicillin-sulbactam combination. These findings are a warning signal to health authorities since Minas cheese is a ready to eat food product, and therefore should not pose health risks to the population.


Freitas M.N.M.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Vale R.F.D.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Fust A.M.B.S.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | Fernandes F.S.,Instituto Nacional Of Controle Of Qualidade Em Saude Incqs Fiocruz | And 2 more authors.
Revista Virtual de Quimica | Year: 2016

Anticoagulant and preserving solutions are designed to maintain the viability of blood and blood components. Plastic bags used for human blood storage are classified as Class III product for health. The RDC No. 35 of June 12, 2014 is the legislation that describes the quality control of blood units in Brazil. According to the resolution of the physical and chemical tests to be performed on anticoagulants solutions is the determination of 5-hydroxymethylfurfural content (5-HMF), a highly toxic pollutant formed by thermal decomposition of glucose present in the solutions, which may lead the patient even to death, justifying the need for their control. This work aimed to optimize and validate the methodology for determining the 5-HMF as recommended by ANVISA and the Inmetro. The parameters analyzed in the analytical validation were: specificity, linearity, detection limit, quantification limit, accuracy, precision and robustness. The results showed that the method is very effective for determining the 5-hydroxymethylfurfural which culminated in their integration into technical regulations published in June 2014.

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