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de Jesus M.S.,State University of Feira de Santana | Rodrigues W.C.,Severino Sombra University | Barbosa G.,Institute Pesquisa Clinica Evandro Chagas | Trilles L.,Institute Pesquisa Clinica Evandro Chagas | And 3 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2012

Cryptococcus neoformans is the most common causative agent of cryptococcosis worldwide. Although this fungus has been isolated from a variety of organic substrates, several studies suggest that hollow trees constitute an important natural niche for C. neoformans. A previously surveyed hollow of a living pink shower tree (Cassia grandis) positive for C. neoformans in the city of Rio de Janeiro, Brazil, was chosen for further investigation. Odontomachus bauri ants (trap-jaw ants) found inside the hollow were collected for evaluation as possible carriers of Cryptococcus spp. Two out of 10 ants were found to carry phenoloxidase-positive colonies identified as C. neoformans molecular types VNI and VNII. The ants may have acted as a mechanical vector of C. neoformans and possibly contributed to the dispersal of the fungi from one substrate to another. To the best of our knowledge, this is the first report on the association of C. neoformans with ants of the genus Odontomachus.

De Filippis I.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | Gopalan V.,National Institute of Allergy and Infectious Diseases | Huyen Y.,National Institute of Allergy and Infectious Diseases
Infection, Genetics and Evolution | Year: 2011

The variable regions (VR) of the surface-exposed PorA protein of Meningococci are used for subtyping and are considered the most abundant epitopes of outer membrane vesicle-based vaccine preparations. We have developed both a database that maintains all the known VR3 alleles and a web-based application for the rapid identification and submission of new VR3 variants based on sequence comparison. © 2010 Elsevier B.V.

Zahner V.,Instituto Oswaldo Cruz | de Carvalho e Silva A.C.T.,Instituto Oswaldo Cruz | de Moraes G.P.,Instituto Oswaldo Cruz | McIntosh D.,Federal Rural University of Rio de Janeiro | de Filippis I.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz
Memorias do Instituto Oswaldo Cruz | Year: 2013

Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species.

Sardinha G.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | Gomes E.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | Romanelli C.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | Andrade C.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | And 2 more authors.
Diagnostic Microbiology and Infectious Disease | Year: 2013

Outbreaks caused by serogroup C meningococci in the northeast region of Brazil from 2005 to 2011 were associated to the emergence of variant ET-15 of cc11, which has been replaced by cc103 from 2006 to date. The increase of cc103 should be closely monitored to prevent the spread of this clone to neighbouring regions. © 2013 Elsevier Inc.

Pereira Baio P.V.,Laboratorio Of Difteria E Corinebacterias Of Importancia Clinica | Pereira Baio P.V.,Instituto Nacional Of Controle Of Qualidade Em Saude Fiocruz | Pereira Baio P.V.,Laboratorio Quimico Farmaceutico do Exercito | Franceschi Mota H.,Laboratorio Of Difteria E Corinebacterias Of Importancia Clinica | And 12 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2013

Corynebacterium striatum is a potentially pathogenic microorganism with the ability to produce outbreaks of nosocomial infections. Here, we document a nosocomial outbreak caused by multidrug-resistant (MDR) C. striatum in Rio de Janeiro, Brazil. C. striatum identification was confirmed by 16S rRNA and rpoB gene sequencing. Fifteen C. striatum strains were isolated from adults (half of whom were 50 years of age and older). C. striatum was mostly isolated in pure culture from tracheal aspirates of patients undergoing endotracheal intubation procedures. The analysis by pulsed-field gel electrophoresis (PFGE) indicated the presence of four PFGE profiles, including two related clones of MDR strains (PFGE I and II). The data demonstrated the predominance of PFGE type I, comprising 11 MDR isolates that were mostly isolated from intensive care units and surgical wards. A potential causal link between death and MDR C. striatum (PFGE types I and II) infection was observed in five cases.

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